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Effects of Frozen Storage, Radiation and Their Combined Treatments on Microorganisms of Freshwater Mola Fishamblypharyngodon Mola

MD. MANSURUL HAQUE
Department of Zoology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.

M. G. SORROWAR
Department of Zoology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.

HARUN-UR- RASHID
Microbiology and Industrial Irradiation Division, Institute of Food and Radiation Biology,Atomic Energy Research Establishment, Savar, Dhaka-1000, Bangladesh.

Abstract/ Executive Summary:

The study revealed that the total viable bacterial count, staphylococcal count, coliform count, faecal coliform count, Aeromonas count and total fungal counts varied from 8.8 x10⁷ to 1.27 x 10⁸, 2.7 x 10⁶ to 4.2 x10⁶, 9.0 x 10⁴to 1.03 x 10⁵, 4.8 10⁵ to 7.3 x 10⁵, 2.7 x 10⁵ to 5.0 x 10⁵ and 1.6x 10³ to 3.5 x 10³ cfu/gm, respectively. Seventy six bacterial strains were isolated and identified, where Staphylococcus aureus, Micrococcus varians, Aeromonas hydrophila, Klebsiella ozaenae, Bacillus subtilis, Escherichia coli, Bacillus megaterium, Klebsiella edwardsii, Pseudomonas aerugenosa and Micrococcus radiodurans were 16 (21.06%), 11 (14.47%), 9 (11.82%), 6 (7.89%), 6 (7.89%), 8 (10.53%), 6 (7.89%), 4 (5.26%), 7 (9.21%) and 3 ( 3.95% ), respectively. Total coliform, faecal coliform, Aeromonas bacteria and total fungi were found to be totally inactivated by the irradiation dose of 2.5 kGy onwards and total viable bacteria and staphylococcal bacteria were eliminated completely at the irradiation dose of 7.5 kGy. The bacterial counts were shown to be gradually decreased in all the samples from initial to final storage. The combined treatments were found more effective than that of the single treatment for reducing or eliminating the associated bacteria. The moisture contents of the studied fish were found nearly similar during initial and final storage periods and also at different radiation doses but the total protein contents decreased during final storage period and also at different radiation doses.

Keywords: Mola fish, Coliform, Microorganisms, Radiation treatment, Frozen storage

Location:

Start Date:

End Date:

Program Area: Postharvest and Agro-processing

Commodity: Fish

Objectives:

To oversee the effectiveness of frozen storage, radiation and their combined treatment in the stated fish which would be an effective means of increasing the shelf life of that fish and other fishes as well.

Methodology:

The fishes were collected from various fish markets of Savar and Nabinagar. All the samples were aseptically packed in pre-sterilized polythene bags supplied from the Laboratory of Microbiology and Industrial Irradiation Division (MIID), IFRB, AERE. The polythene bags were sealed air tight after collecting the samples. The samples were immediately transported to the laboratory in chilled condition (in insulating foam box with ice) for microbiological analysis. Around 80 polythene bags were prepared and they were sealed properly with the help of sealer machine. Then they were irradiated in the Gamma Irradiation Laboratory to make them aseptic. For six-month-sampling, six sets of polythene bags were prepared. In each polythene bag 200 gram sample was taken. The plate count was performed following the method of Sharp and Lyles (1969). The media used were nutrient agar (pH 7.0 - 7.4), MacConkey agar, mFC agar, staphylococcal agar, potato dextrose and starch ampicillin agar media for total viable bacterial count (TVBC), total viable coliform count (TCC), total faecal coliform count (TFCC), total staphylococcal count (TSC), total fungal count (TFC) and total Aeromonas count (TAC), respectively. Plates were incubated at 37ºC for 24 hrs and the count was expressed as colony forming unit per gram. Various morphological characteristics were observed and biochemical tests were performed for the isolation and identification of microorganisms. All the bacterial isolates were identified according to the Bergey’s Manual of Determinative Bacteriology (1974). For fungal count, the plates were incubated at 28ºC and counts were recorded after 5 days of incubation. For determination of the moisture content of the sample porcelain made crucible was heated at 105^oC for 1 hr and then kept at desiccators until the crucible reached at room temperature, 4 gm of sample was taken in the crucible and dried at 105^oC for 3 hrs. Then the sample was taken at desiccators until it reached at room temperature and weight of the sample was taken. The process was continued until constant weight was obtained. The loss of moisture from the sample was then determined and expressed in percentage using the following formula.. Moisture content ( % )= Initial weight -Final weight/ Original weight of sample x100.

Source of Information: Journal of Bangladesh Academy of Sciences, Vol. 37, No. 1, 21-31, 2013

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The moisture contents of the studied fish were found nearly similar during initial and final storage periods and also at different radiation doses but the total protein contents decreased during final storage period and also at different radiation doses.

Knowledge Management: Journal