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Effect of Rhizobium Inoculant and Phosphorus on the Growth and Yield of BINAmoog-4

M. Hassan
Department of Soil Science, Bangladesh Agricultural University, Mymensingh-2202

M.H. Shohel
Exim Bank, Agricultural UniversityBangladesh, Chapainawaabganj

M.A. Tarafder
Soil science Division, BINA, Mymensingh

Shahidullah
Institute of Agribusiness and Development

M.H. Mian
Department of Soil Science, Bangladesh Agricultural University, Mymensingh-2202

Abstract/ Executive Summary:

A field experiment was conducted at the farm of Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh during November 2013 to January 2014 to study the effect of biofertilizer and chemical fertilizers on nodulation, growth and yield of mungbean cv. Binamoog-4. The treatments were T₁: control (without any fertilizer), T₂: Rhizobium inoculant, T₃_: Rhizobium inoculant +P ,T₄: Rhizobium inoculant +K+P+N,and T₅:Rhizobium inoculant+P+K. The experiment was laid out in a Randomized Completely Block Design (RCBD) with three replications. The results recorded for nodulation, growth and yield showed significant variation due to single or interaction effect of biofertilizer and chemical fertilizer. Biofertilizer application with chemical fertilizer produced higher dry weight of shoot, nodule, root, leaf as well as higher yield of grain and straw compared to those of uninoculated treatments. Among the treatments, T₄: Rhizobium inoculant+N+P+K produced the tallest plants(52.20 cm), maximum nodule number (29), maximum root length(14.43 cm) and leaf production(25.16 plant^-1) as well as highest grain yield (1441.62 kg ha^-1) and straw yield(1574.33 kg ha^-1) of mungbean. Positive interaction effect of biofertilizer and chemical fertilizer was found on nodulation and yield of mungbean. Thus,it is seen that the use of biofertilizer with N,P,K would ensure higher production of mungbean in AEZ 9 (Agro-ecological-zone no.9) i.e. Old Brahmaputra Floodplain..

Keywords: Rhizobium, Inoculant, Phosphorus, Binamoog-4.

Location: Farm of Bangladesh Institute of Nuclear Agriculture (BINA) head quarter farm,Mymensingh

Start Date: 00-11-2013

End Date: 00-01-2014

Program Area: Crop-Soil-Water Management

Commodity: Mungbean

Objectives:

1. To find out the effect of biofertilizer on the growth and yield performance of mungbean.

Methodology:

The experiment was conducted at farm of Bangladesh Institute of Nuclear Agriculture (BINA) head quarter farm,Mymensingh;under Old Brahmaputra Floodplain(AEZ 9) during November to January 2013-2014. The crop used in this study was mungbean (Vigna radiata L.) Binamoog-4.There were five treatments in different combinations of biofertilizer and N,P,K replicated thrice in randomized complete block design.The Rhizobium strain was inoculated into yeast mannitol broth medium containing 250 ml Erlenmeyer flask under laminar flow cabinet. The inoculated flask was then placed on an electric shaker for seven days for optimal growth of Rhizobial cell in the broth culture. After seven days, the bacterial culture was tested its purity and growth. The Rhizobial cell was counted through plate count method. The population was maintained above 10 cells per ml broth culture. The populations of Rhizobium cells were 1.9x10⁹, 2.0x10⁹ and 2.1x10⁹ per ml broth culture respectively. All the chemical fertilizers except nitrogenous fertilizer were mixed with soil at the rate of nitrogen 8kg ha^-1 as urea,phosphorus 14kg ha^-1 as TSP,potassium 18 kg ha^-1 as MOP thoroughly at the time of final land preparation and urea was applied in two installments at 30 and 45 DAS. Five plant samples were collected from the field at 35 and 55days of sowing and at harvest, from each plot, at random and were carefully uprooted with the help of a nirani cuttin into a soil block so that no nodules were left in the soil. The soil blocks were kept for sometime in water buckets to soak before gently washing out the soil from the roots. The roots were then finally washed carefully with water. The nodules from root of each plant were collected. The length of the roots and shoots of each plant were recorded. Initial soil samples were collected and analyzed at soil science laboratory BINA, following standard procedure.

Source of Information: J. Agrofor. Environ. 8 (2): 181-183, 2014; ISSN 1995-6983

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Overall results of the field experiment showed that biofertilizer (Rhizobium inoculant) in combination with chemical fertilizers(N + P + K) were beneficial for growth, yield and yield contributing characters of mungbean variety (BINAmoog4). Considering the obtained result it may be inffered that: (i). Such study is needed in different agro-ecological zones (AEZs) of Bangladesh for regional adaptability and other performance, (ii). Combine effect of nitrogen, phosphorus and potassium with biofertilizer appeared promising for obtaining the higher yields of mungbean.

Knowledge Management: Journal