Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Evaluation of Different Pathogenic Isolates of Fusarium Oxysporum on Lentil

M A Kashem
Pathology Division , Bangladesh Institute of Nuclear Agriculture, Mymensingh

I. Hossain
Pathology Division , Bangladesh Institute of Nuclear Agriculture, Mymensingh

Md.Jalaluddin
Pathology Division , Bangladesh Institute of Nuclear Agriculture, Mymensingh

MAR Hawlider
Departmentof Plant Pathology, BAU, Mymensingh

Abstract/ Executive Summary:

Twelve isolates of Fusarium oxysporum co ll ected fro m different location s of Bangladesh sh were evaluated for growth , colony characters a nd pathogenicity on lentil at the laboratory and glasshouse. Almost all the colonies of the isolates of Fusarium oxysporum grown on PDA were found to be regular, whitish-cream and floccose in texture. The highest radial growth (89.83mm) of mycelium after 6 days of inoculation was recorded in the isolate FBg-1. In pot tnal, the highest pre-emergence death (30%) and foot and root rot (53%.) were recorded in the isolates FL-4 and FBg-1 , respectively which were virulent to Barimasur-4 . The highest per cent decrease of germinal ion and per cent increase se of foot and root rot over control l was recorded in the i isolate Fl-4 an d FBg-1 , respectively. These two isolates were more aggressive to lentil that could be used for screening lentil germplasms /strains to identityfy the sources of resistance to foot and root rot diseases.

Keywords: Fusarium oxysporum , Lentil

Location: Bangladesh Institute of Nuclear Agriculture, Mymensingh

Start Date: 00-00-2002

End Date: 00-00-2004

Program Area: Pest Management

Commodity: Lentil

Objectives:

To identify the morphological virulent isolates(s) of Fusarium oxysporum causing foot and root rot of available in soils at different locations of Bangladesh.

Methodology:

The experiments were carried out in the laboratory and glasshouse of the Plant Pathology Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh, Bangladesh during the period of 2002-2004 following CRD with five replications. Collection, isolation, purification and preservation of isolates oxysporum: Plant samples of various leguminous plants with foot and root rot symptoms were collected from different cropping areas in Bangladesh .The samples showing typical foot and root rot symptoms were brought to the laboratory, washed initially with tap water to remove sand and oil particles and cut into small pieces (1.0 cm) along with healthy and dead tissues. Surface sterilization of cut pieces (inocula) was done with 1:1000 mercuric chloride solution for 1 minute. Then the plant pieces were washed thrice with sterilized water and placed on sterilized filter paper to remove excess water adhering to the pieces. Thereafter, five pieces were plated in PDA plates aseptically maintaining equal distances (Begum et al. 1998). The plates were incubated at 28 ± l ° C temperature for 7 days and observation was made regularly to see the growth of fungi from the plant pieces. The fungus was then purified by hyphal tip culture technique (Tuite 1969). The isolated fungus was identified following the key outlined by Booth ( 1971) and Singh (1982). The pure culture of the isolates of Fusarium oxysporum were preserved in PDA slants at 5 ± I °C in refrigerator as stock culture for future use (Begum, 1997). pieces. Thereafter, five pieces were plated in PDA plates aseptically maintaining equal distances (Begum et al. 1998). The plates were incubated at 28 ± l ° C temperature for 7 days and observation was made regularly to see the growth of fungi from the plant pieces. The fungus was then purified by hypha! tip culture technique (Tuite 1969). The isolated fungus was identi fled following the key outlined by Booth ( 1971) and Singh (1982). The pure culture of the isolates of Fusarium oxysporum were preserved in PDA slants at 5 ± I °C in refrigerator as stock culture for future use (Begum, 1997). The colony growth, sporulation and colony characteristics of different Isolates of Fusarium oxysporum were studied on PDA. The growth study of different isolates of Fusarium oxysporum were carried out by using PDA following the method of Begum et a!. (1998). A 5mm block of each isolate was placed at the centre of the five replicated petriplates. The linear mycelial gr Twelve isolates of Fusarium oxysporum were tested by soil infestation for their pathogenicity in pot trials in the glasshouse. The dried soil mixed with well decomposed cowdung at 2:1 (soil: cowdung) were sterilized with formalin (40%) at the rate of 5 ml formalin diluted with 20 ml of water for 4kg soil. The formalin treated soil was covered with polythene sheet for 48-hrs and then exposed to 48- hrs aeration before pot filling with 2kg soil/pot (Dashgupta, 1988). Pot soil was inoculated with F. oxysporum previously grown in chickpea bran at the rate of 40 g/pot before 3 days of sowing seeds (Vyas and Mathur, 2002). Soil was moistened to 50% water holding capacity. Seeds of lentil variety, Barimashur-4 were sown ( 15 seeds per pot) with four replications and the control pots were sown using sterilized soil without inoculation of the pathogen. Disease incidence was observed regularly and recorded at 5, 10, 15, 20, 25 and 30 days after sowing to estimate the effect of Fusarium oxysporum on pre-emergence and post emergence death of seedling. The causal agent of death of seedlings was con firmed after re isolation of the pathogen. The data on seed germination, pre emergence death, foot and root rot/wilt incidence and the plant stand were recorded (Begum, 2003

Source of Information: Bangladesh J. Nuclear Agric. 21 & 22 : 53-62 , 2006; ISSN 0258-7130

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The highest radial growth (89.83mm) of mycelium after 6 days of inoculation was recorded in the isolate FBg-1. In pot triall, the highest pre-emergence death (30%) and foot and root rot (53%.) were recorded in the isolates FL-4 and FBg-1 , respectively which were virulent to Barimasur-4 . The highest per cent decrease of germinal ion and per cent increase se of foot and root rot over control l was recorded in the i isolate Fl-4 an d FBg-1 , respectively. These two isolates were more aggressive to lentil that could be used for screening lentil germplasms / strains to identityfy the sources of resistance to foot and root rot diseases.

Knowledge Management: Journal