Agricultural Research Management Information System

  • Home
  • Research Summary
    • All
    • Government Organization
      • Agriculture Training Institute, Ishwardi, Pabna
      • Bangabandhu academy for poverty alleviation and rural development (BAPARD)
      • Bangabandhu Sheikh Mujibur Rahman Science & Technology University
      • Bangladesh Bureau of Statistics
      • Bangladesh Institute of Health Sciences
      • Bangladesh Institute of Tropical & Infections Diseases (BITID)
      • Bangladesh Meteorological Department
      • Bangladesh National Herbarium
      • Bangladesh Space Research and Remote Sensing Organization
      • Bangladesh Technical Educational Board
      • Barind Multipurpose Development Authority
      • Central Cattle Breeding Station
      • Department of Agriculture Extension
      • Department of Fisheries
      • Department of Livestock Services
      • Department of Youth Development
      • Dhaka Medical College
      • Geological Survey of Bangladesh
      • Institute of Epidemiology, Disease Control & Research
      • Jatiya Kabi Kazi Nazrul Islam University
      • Khulna Govt. Women College
      • Livestock Training Institute
      • Local Government Engineering Department
      • Ministry of Agriculture
      • Ministry of Environment and forest
      • Ministry of Fisheries and Livestock
      • Ministry of Labour & Employement
      • Ministry of Land
      • Ministry of Public Administration
      • Ministry of Textiles and Jute
      • Ministry of Water Resources
      • Ministry of Youth and Sports
      • National Agricultural Training Academy
      • National institute of preventive and social medicine
      • National Mushroom Development and Extension Centre
      • Pabna University of Science and Technology
      • Seed Certification Agency
      • Shaheed Suhrawardy Medical College
      • Sheikh Hasina University
      • University Grants Commission
      • Youth Training Centre
    • Autonomous/Semi-gov Org
      • Bangladesh Academy for Rural Development
      • Bangladesh Agricultural Development Corporation
      • Bangladesh Atomic Energy Commission
      • Bangladesh Council of Scientific and Industrial Research
      • Bangladesh Fisheries Development Corporation
      • Bangladesh Institute of Development Studies
      • Bangladesh Institute of Management
      • Bangladesh Milk Producers Cooperative Union Limited
      • Bangladesh Water Development Board
      • BIRDEM
      • Center for Environmental and Geographic Information Services
      • Hortex Foundation
      • Institute of Water Modeling
      • National Institute of Biotechnology
      • River Research Institute
      • Rural Development Academy
    • NARS
      • Bangladesh Agricultural Research Council
      • Bangladesh Agricultural Research Institute
      • Bangladesh Fisheries Research Institute
      • Bangladesh Forest Research Institute
      • Bangladesh Institute of Nuclear Agriculture
      • Bangladesh Jute Research Institute
      • Bangladesh Livestock Research Institute
      • Bangladesh Rice Research Institute
      • Bangladesh Sericulture Research and Training Institute
      • Bangladesh Sugarcrop Research Institute
      • Bangladesh Tea Research Institute
      • Bangladesh Wheat and Maize Research Institute
      • Cotton Development Board
      • Soil Resource Development Institute
    • Public University
      • Ahsanullah University of Science and Technology
      • Bangabandhu Sheikh Mujibur Rahman Agricultural University
      • Bangamata Sheikh Fojilatunnesa Mujib Science and Technology University
      • Bangladesh Agricultural University
      • Bangladesh Open University
      • Bangladesh University of Engineering and Technology
      • Bangladesh University of Professionals
      • Bangladesh University of Textiles
      • Barisal Government Veterinary College
      • Begum Rokeya University
      • Chittagong University of Engineering and Technology
      • Chittagong Veterinary and Animal Science University
      • Comilla University
      • Dhaka University of Engineering & Technology
      • Dinajpur Government Veterinary College, Dinajpur
      • Gono Bishwabidyalay
      • Hajee Mohammad Danesh Science and Technology University
      • Islamic University, Kushtia
      • Jagannath University
      • Jahangirnagar University
      • Jessore University of Science and Technology
      • Jhenaidha Government Veterinary College
      • Khulna Agricultural University
      • Khulna University
      • Khulna University of Engineering & Technology
      • Mawlana Bhashani Science and Technology University
      • Millitary Institute of Science and Technology
      • National University
      • Noakhali Science and Technology University
      • Patuakhali Science and Technology University
      • Rajshahi University of Engineering and Technology
      • Shahjalal University of Science & Technology
      • Sher-e-Bangla Agricultural University
      • Sylhet Agricultural University
      • Sylhet Government Veterinary College
      • University of Barisal
      • University of Chittagong
      • University of Dhaka
      • University of Rajshahi
    • Private University
      • Asian University of Bangladesh
      • Atish Dipankar University of Science and Technology
      • BGC Trust University Bangladesh
      • BGMEA University of Fashion & Technology (BUFT)
      • BRAC University
      • City University
      • Daffodil International University
      • East West University
      • Exim Bank Agricultural University
      • Gana Bishwabiddalaya
      • Hamdard University
      • Independent University, Bangladesh
      • International Islamic University Chittagong
      • International University of Business Agriculture and Technology
      • Islamic University of Technology
      • Leading University, Sylhet
      • North South University
      • Premier University
      • Primeasia University
      • Private University
      • SOAS, University of London
      • Southeast University
      • Stamford University
      • State University of Bangladesh
      • The Millenium University
      • University of Asia Pacific
      • University of Development Alternative
      • University of Information Technology and Sciences
      • University of Liberal Arts Bangladesh
      • University of Science and Technology, Chittagong
      • World University
    • INGO/IO/NGO/Private Org
      • ACI Limited
      • Agricultural Advisory Society (AAS)
      • Apex Organic Industries Limited
      • Arannayk Foundation
      • Bangladesh Academy of Sciences
      • Bangladesh Centre for Advanced Studies
      • Bangladesh Institute of Social Research
      • Bangladesh Science Foundation
      • Bangladesh Unnayan Parishad
      • BAPA
      • BRAC
      • CARE Bangladesh
      • CARITAS
      • Centre for Environmental Geographical Information System
      • Centre for Policy Dialogue (CPD)
      • Creative Conservation Alliance
      • Dhaka Ahsania Mission
      • Dwip Unnayan Sangstha
      • EMBASSY OF DENMARK, BANGLADESH
      • Energypac Limited Bangladesh
      • FAO- Bangladesh
      • FIVDB
      • ICDDRB, Mohakhali, Dhaka-1212
      • iDE Bangladesh
      • Innovision Consulting Private Ltd.
      • International Center for Climate Change and Development
      • International Centre for Integrated Mountain Development
      • International Development Research Centre
      • International Fertilizer Development Center, Bangladesh
      • International Food Policy Research Institute
      • International Maize and Wheat Improvement Centre
      • International Potato Center
      • IRRI- Bangladesh
      • IRRI-Philippines
      • Ispahani Agro LTD
      • IUCN, Bangladesh
      • Krishi Gobeshina Foundation
      • Lal Teer
      • Mennonite Central Committee
      • Metal (Pvt.) Ltd
      • Modern Herbal Group
      • Palli Karma-Sahayak Foundation
      • Practical Action Bangladesh
      • Proshika
      • RDRS Bangladesh
      • RIRI-Philippines
      • Rothamsted Research
      • SAARC Agricultural Centre
      • SAARC Meteorological Research Centre
      • Social Upliftment Society
      • South Asia Enterprise Development Facility
      • Square Pharmaceuticals Ltd.
      • Supreme Seed
      • Transparency International Bangladesh
      • Unnayan Onneshan
      • USAID
      • Water Resources Planning Organization
      • Winrock International
      • World Bank
      • World Food Program
      • World Vegetable Center
      • WorldFish Centre, Bangladesh
    • Foreign University
      • Asian Institute of Technology
      • Auckland University of Technology
      • Australian National University
      • Bidhan Chandra Krishi Viswavidyalaya
      • BOKU-University of Natural Resources and Applied Life Sciences
      • Cranfield University
      • Curtin University
      • Foreign University/ Institute
      • Hiroshima University
      • Hokkaido University
      • Huazhong Agricultural University
      • International Islamic University, Malaysia
      • Kagawa University
      • Kangwon National University
      • Kochi University
      • Kyoto University
      • Kyushu University
      • Ladoke Akintola University of Technology
      • Murdoch University
      • Nagoya University
      • NOAA-CREST, CCNY
      • Royal Veterinary and Agricultural University
      • San Diego State University
      • Shinshu University
      • Tottori University
      • United Nations University
      • University Malaysia Kelantan
      • University Malaysia Pahang
      • University Nova de Lisboa
      • University of Alberta
      • University of Bremen
      • University of Bremen
      • University of Calgary
      • University of california
      • University of Greenwich
      • University of Hamburg, Hamburg
      • University of Hannover
      • University of Hawaii
      • University of Helsinki, Finland
      • University of Kalyani
      • University of Leeds
      • University of Liverpool
      • University of Malaya
      • University of Milan
      • University of New England
      • University of Philippines
      • University of Plymouth
      • University of Queensland
      • University of Reading
      • University of Southampton
      • University of Texas
      • University of the Punjab
      • University of Tokyo
      • University of Toronto
      • University of Wales
      • University of Washington
      • University of Wollongong
      • University Putra Malaysia
      • University Sains Malaysia
  • Search
    • Search by Keyword
    • Search by Organization
    • Search by Program Area
    • Search by Commodity/Non-commodity
    • Search by Funding Source
    • Search by Researcher
    • Custom Search
    • On-going Research
  • About Us
    • ARMIS
    • Brochure
  • Contact Us
    • BARC Personnel
    • ARMIS Personnel
    • Feedback
  • Report
    • All
    • By Organization
      • Bangladesh Agricultural Research Council
      • Bangladesh Agricultural Research Institute
      • Bangladesh Fisheries Research Institute
      • Bangladesh Forest Research Institute
      • Bangladesh Institute of Nuclear Agriculture
      • Bangladesh Jute Research Institute
      • Bangladesh Livestock Research Institute
      • Bangladesh Rice Research Institute
      • Bangladesh Sericulture Research and Training Institute
      • Bangladesh Sugarcrop Research Institute
      • Bangladesh Tea Research Institute
      • Bangladesh Wheat and Maize Research Institute
      • Cotton Development Board
      • Soil Resource Development Institute
    • Research Trend Analysis
  • User Request
  • Data Input
  • Help
    • Operation Manual
      • PDF
      • Video
    • Program Area & Commodity
  • We have reached 37600 number of research entries at this moment.
    • Logout

Research Detail

  1. Home
  2. Research
  3. Detail
R. H. Sarker
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Barkat Murtaja Mustafa
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Maximum shoot regeneration in two indigenous potato (Solanum tuberosum L.) was observed on MS semi-solid medium supplemented with 1.0 mg/l BAP and 0.1 mg/l GA3. Among the two varieties, namely Lal Pakri and Jam Alu the former showed best response in terms of number of shoots/explant, nodes/shoot and shoot length. Half strength of MS containing 0.1 mg/l IAA was found to be best for root induction from the excised shoots. Two strains of Agrobacterium namely, LBA4404 containing pBI121 and EHA105 having plasmid pCAMBIA1301 were used for transformation. Among the two strains, LBA4404 with the vector pBI121 showed better response. In the nodal segment of Lal Pakri, the transformed zone coincided with the regeneration point. Regeneration from transformed tissue was obtained on MS supplemented with 1.0 mg/l BAP and 0.1 mg/l GA3. Histochemical GUS assay showed the expression of GUS gene in the leaf tissues of the transformed shoot.

  Micropropagation, Indigenous, Potato, Genetic transformation, Agrobacterium
  Bogra and Mymensingh districts, Bangladesh
  
  
  Variety and Species
  Potato

To optimize various factors influencing reproducible transformation protocols in two indigenous potato varieties using two strains of Agrobacterium containing different vectors.

Two indigenous potato (Solanum tuberosum L.) varieties, namely Lal Pakri and Jam Alu were used in the present study. The seed tubers of these two varieties were collected from the local growers of Bogra and Mymensingh, respectively. The tubers were kept in an incubator at 180C for sprouting. About 4.0 - 5.0 cm long sprouts were cut and washed in distilled water. They were surfaced sterilized with 0.1 % HgCl2 solution (w/v) for 15 min followed by several washes with sterilized distilled water. About 1.0 cm long sprout segments containing one to two nodes were used as explants and cultured on agar solidified MS medium supplemented with various combination of BAP, Kn and GA3 for induction and proliferation of multiple shoots. pH of the medium was adjusted to 5.8 before autoclaving. The culture vessels containing the explants were incubated in the growth room under 16/8 hrs light/dark cycle at 25 ± 20C. For induction of roots, regenerated shoots (3 - 4 cm long) were excised and transferred to half strength of MS medium supplemented with different concentrations of IAA, IBA and NAA. Agrobacterium tumefaciens strain LBA4404 harboring the binary plasmid pBI121 and strain EHA105 containing the plasmid pCAMBIA1301 were used in the present study. Both the plasmids contain the GUS reporter gene. The plasmid pBI121 contains the plant selectable marker gene nptll conferring resistance to kanamycin and the plasmid pCAMBIA1301 contains the plant selectable marker gene conferring resistance to hygromycin, hptII. Leaf, internodal and nodal segments from in vitro grown planlets were used in the genetic transformation. The Agrobacterium suspension was centrifuged at 10000 rpm for 10 min and the precipitate was resuspended in appropriate volume of MS medium so that the density of bacterial suspension becomes 0.8 - 1.0 (at 600 nm). The cut explants were then immersed in the resuspended Agrobacterium suspension for different periods of time, blotted dry and cocultured for three days in the dark on MS medium with 1 mg/l BAP and 0.1 mg/l GA3. After coculture the explants were washed in cefotaxime (500 mg/l) to kill the Agrobacterium. They were then cultured on regeneration medium. Following cocultivation the explants were subjected to transient GUS histochemical assay according to Jefferson (1987) to monitor the efficiency of infection. The cocultivated explants were incubated for 24 - 48 h at 370C in the substrate X-gluc (5-bromo, 4-chloro, 3-indolyl-glucuronide) and subsequently bleached with 70% ethanol before scoring for transient GUS expression.

  Plant Tissue Cult. 12(1) : 69-77, 2002 (June)
  
Funding Source:
  

Transformed tissue were obtained from leaf, nodal and internodal segments. In both the potato varieties leaf explants showed best transformation ability followed by internodal and nodal segments. Histochemical GUS assay revealed the expression of GUS reporter gene in the leaf, basal callus and nodal segments in Lal Pakri. It was evident that the transformed sectors of the axillary buds coincided with the area of regeneration. Regenerated shoots from the transformed tissues showed numerous blue sectors in the leaf and newly developed shoot bud. However, future experiments are required to regenerate fully transformed plantlets showing stable integration of GUS and nptII gene. The results of the present investigation demonstrated the development of efficient in vitro regeneration systems in two local potato varieties, namely Lal Pakri and Jam Alu. Encouraging results of transformation experiments have shown that it may be develop disease resistant varieties of potato through genetic transformation.

  Journal
  


Copyright © 2025. Bangladesh Agricultural Research Council.