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Organogenesis in Teasle Gourd (momordica Dioica Roxb.)

S. A. Nabi
Country Office, IRRI, Banani, Dhaka, Bangladesh

M. M. Rashid
Tuber Crops Research Centre, BARI, Gazipur-1701, Bangladesh.

M. Al-Amin
Biotechnology Division, BARI, Gazipur-1701, Bangladesh.

M. G. Rasul
Department of Genetics & Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur-1703, Bangladesh

Abstract/ Executive Summary:

Of the four types of explants of teasle gourd (Momordica dioica Roxb.) viz. node, shoot tip, leaf and the cotyledon, the cotyledon showed the best performance. The combination 1.0 mg/l BAP and 0.1 mg/l NAA was found most suitable in callus induction followed by 0.2 mg/l NAA. The highest number of multiple and tallest shoots were obtained on MS medium fortified with 1.0 mg/l BAP and 0.1 mg/l NAA. For rooting, half strengh MS supplemented with IBA proved to be better than IAA, although on half strnegth MS supplemented with IAA tallest shoots were observed.

Keywords: Organogenesis, Micropropagation, Momordica dioica

Location: All over Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Teasle Gourd

Objectives:

To develop an efficient protocol for in vitro plant regeneration of teasle gourd and to select suitable explants for in vtiro propagation.

Methodology:

As explants shoot tip, leaves and nodes were taken from field grown mature plants. MS medium and different concentrations of growth regulators such as BAP, NAA, IAA and IBA were used. Explants were washed thoroughly under running tap water in plastic pot for 10 to 15 min. Later plants were cut into pieces and washed with detergent powder (Wheel brand) and 1 - 2 drops of Tween-20 for 10 min and then sterilized with 0.1 % HgCl₂solution along with 1 - 2 drops of Tween-20 for 5 min followed by three - four rinses in autoclaved distilled water to remove traces of HgCl₂ under a laminar airflow cabinet. Small segments (0.5 - 1.0 cm) were cultured on MS supplemented with specific concentration of growth regulators (BAP and NAA) in combination adding 3 % high quality sugar (SOS brand, Singapore) and 0.7 % agar, pH was adjusted and maintained at 5.8. Subcultures were done every 30 days interval. Cultures were kept for callus induction and maintenance; shoot initiation, proliferation and elongation. Each proliferated and adventitious shoot was cut from the basal end and subcultued again for further multiple shoot induction. Regenerated multiple shoots were cut and individual shoots were placed in half strength MS containing different concentrations of IBA and IAA for root induction. All cultures were kept at a temperature of 25 ± 1⁰C under 16 h photoperiod at 2000 - 3000 lux from fluorescent tubular lamps. The experiment was conducted under controlled conditions and followed complete randomized design (CRD) with three replications. Duncan's Multiple Range Test (DMRT) was used with the help of MSTAT software.

Source of Information: Plant Tissue Cult. 12(2) : 173-180, 2002 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The present investigation reveals that auxin; IBA is better than IAA in terms of rooting ability. On the other hand, the number of roots per plants was lower than that of the findings. This might be due to the genotypic variation of the explants and along with the cultural and environmental conditions. For the establishment of plant, regenerated healthy rooted plantlets were placed at room temperature for one - two weeks. Then the plantlets were removed from the culture bottle and carefully cleaned the plantlets to remove adhering agar. Plantlets were sprayed with fungicide and planted to normal and sterilized soil to observe the accomplishment of the planted media in polyethylene bag. The polyethylene bags were kept in the net-house. It was observed that sterilized soil showed relatively cent per cent survivability than normal soil (83.33 %). Nevertheless, sterilization is a costly process. Thus, normal soil is more preferable for the practical purpose. Before going to transplant seedling in polyethylene bag, hardening is essential. To preserve moisture, watering is allowed as and when necessary. After 25 days of hardening, seedlings were ready for planting in the field.

Knowledge Management: Journal