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In Vitro Propagation of Plum (zyziphus Jujuba Lam.)

S. N. Hossain
Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar, G.P.O. Box 3787, Dhaka, Bangladesh

M. K. Munshi
Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar, G.P.O. Box 3787, Dhaka, Bangladesh

M. R. Islam
Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar, G.P.O. Box 3787, Dhaka, Bangladesh

L. Hakim
Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar, G.P.O. Box 3787, Dhaka, Bangladesh

M. Hossain
Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar, G.P.O. Box 3787, Dhaka, Bangladesh

Abstract/ Executive Summary:

Shoot tips of seedlings raised in vitro from sterilized seeds of Zyziphus jujuba Lam. were used as primary explants. A combination of 0.5 mg/l BA and 0.2 mg/l NAA in MS medium resulted highest number of multiple shoots. Maximum elongation of shoots was also obtained in the same combination. A concentration of 1.0 mg/l IBA in half strength of MS proved to be the best for rooting. In vitro regenerated plantlets were successfully transferred to soil under natural environment.

Keywords: In vitro, Shoot tip, Propagation, Zyziphus jujuba

Location: Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar, Dhaka, Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Plum

Objectives:

To develop a protocol for in vitro multiplication of plum with shoot tip explant as an initial plant material.

Methodology:

Sterilized seeds of plum (Zyziphus jujuba Lam.) were germinated in vitro on MS medium with 3% sucrose without growth regulators. Shoot tips of in vitro raised plum were cultured on MS supplemented with different concentration of BA and Kn singly or in combination with NAA. The medium was adjusted to pH 5.8 and solidified by 3.6 g/l phytagel. The medium was autoclaved at 121⁰C and at 1.2 kg/cm² pressure for 20 min. For subsequent subcultures BA and Kn in the concentration ranging from 0.5 to 2.0 mg/l singly and also in combination with a constant concentration of 0.2 mg/l NAA were added to the basal medium for induction of multiple shoots. For root induction, MS medium at half strength with 0.2 - 2.0 mg/l either IBA, IAA or NAA was used. After inoculation, all cultures were grown under a photoperiod of 16 h light from white fluorescent tubes at a temperature of 26 ± 2⁰C. The data were recorded after eight weeks of culture.

Source of Information: Plant Tissue Cult. 13 (1) : 81-84, 2003 (June)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

After four weeks of culture, shoot tip explants showed different responses in production and development of multiple shoots when cultured on MS with different concentrations of BA and Kn singly or in combination along with 0.2mg/l NAA. Shoot tips provide superior explants compared to adventitious buds in the vegetative propagation because of the reduced risk of genetic instability. Among the various hormonal concentrations and combinations used in the present study, 0.5 mg/l BA and 0.2 mg/l NAA were found to be best to yield the highest number of multiple shoots. The highest number of multiple shoots per explant recorded were six. The maximum length of shoots was also obtained in this combination. The combined effect of BA and NAA towards multiple shoot induction was also reported. Among the three auxins (IBA, IAA and NAA) tested for root induction, 1.0 mg/l IBA was found more effective in root production compared to others. Inclusion of either NAA or IAA (0.5 - 2.0 mg/l) in the medium induced low rate of rooting. Rooted plantlets were planted in polythene bags filled with 1 : 1 non-sterile garden soil and compost and successfully acclimatized in natural condition through a gradual increase of the sunlight period. When hardened, these were transferred to soil. Eighty per cent of the plant-lets produced from in vitro cultures survived in ex vitro condition. The findings of the present investigation present a reproducible plant regeneration system through direct organogenesis from shoot tip explant in Zyziphus jujuba.

Knowledge Management: Journal