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In Vitro Clonal Propagation of Paederia Foetida L. - a Medicinal Plant of Bangladesh

M. N. Amin
Department of Botany, University of Rajshahi. Rajshahi-6205, Bangladesh

M. M. Rahman
Department of Botany, University of Rajshahi. Rajshahi-6205, Bangladesh

M. S. Manik
Department of Botany, University of Rajshahi. Rajshahi-6205, Bangladesh

Abstract/ Executive Summary:

Paederia foetida L. is an important perennial climbing medicinal herb. It becomes woody with age. Mass collection of the plant from natural habitats has led to its depletion. We have established a protocol for its mass propagation that would help cultivation of this valuable plant in the homestead. Shoot tips and nodal segments from field-grown plants were used as explants and cultured on MS medium with 1.0 mg/l BA. Proliferation of shoots took place from axillary buds. Subculture of the nodal segments from initial culture on fresh medium gave a higher number of shoots. For rooting, shoots were excised from the proliferating cultures and implanted individually on root induction medium, consisting of half strength MS salts supplemented with 0.1 mg/l of IBA. Within five weeks of transfer, there was good rooting in 95% shoot cuttings. Plantlets were transferred to plastic pots maintained within a polythene tent. After two weeks of acclimatization they were transferred to the open environment where 80% of the plantlets survived.

Keywords: In vitro, Clonal propagation, Paederia foetida

Location: Department of Botany, University of Rajshahi. Bangladesh

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

To establish a protocol for in vitro clonal propagation of P. foetida.

Methodology:

Tender twigs were collected from field grown mature plants of P. foetida L. defoliated and sectioned into 2 - 3 nodal segments. They were washed under continuous flashing of running tap water for 30 min and then treated with a solution of the Savlon (5% v/v) for 10 min and finally surface sterilized with HgCl₂ (0.1% w/v) for 10 min. Lastly, the material was washed three times with autoclaved distilled water to remove any trace of HgCl₂. The shoot tip and nodal segments were excised from the disinfected material and divided into 1.0 - 1.5 cm pieces with at least one node in each explant. The basal medium used for all the experiments was MS formulation containing 30 g/l sucrose, 7 - 8 g/l agar and supplemented with BA and Kn, either individually or in different combinations with auxins, NAA, IBA or IAA. The media were adjusted to pH 5.7 ± 0.1 and autoclaved at 1.1 kg/cm² for 20 min at 121⁰C. Cultures were incubated at 25 ± 1⁰C with a photoperiod of 16 h at 2000 - 3000 lux of cool white fluorescent light. Cultures were initiated in 150 ∇ 25 mm glass tube and subcultured regularly on fresh medium at four-week intervals in 100 ml flasks. The shoots that proliferated from primary explants were isolated and subcultured on fresh medium several times for bulking up shoot culture material. Shoots (3 - 4 cm) were excised from proliferating cultures and implanted on half strength MS supplemented with either of NAA, IBA or IAA (0.1 - 1.0 mg/l) for rooting. Rooted shoots were transferred to pots under ex vitro condition after proper hardening.

Source of Information: Plant Tissue Cult. 13 (2) : 117-123, 2003 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The findings are in agreement with those observed in other plant species such as Vitis vinifera and Adhatoda vasica. About 80 - 95% of the regenerated plantlets could tolerate and survive under ex vitro environment or field conditions. A number of plantlets were lost due to damping off and necrosis during acclimatization in ex vitro condition. Loss of regenerants due to such symptoms was also observed in Eucalyptus tereticornis, Solanum nigrum, Rauvolfia serpentina and Rosa damascena. Through this study a protocol for regeneration of complete plantlets has been established. This is perhaps the first report on in vitro plant regeneration of Paederia foetida Linn. The results may be of some importance as a pioneering study on tissue culture of this medicinal plant. For rooting, shoots were excised from the proliferating cultures and implanted individually on root induction medium, consisting of half strength MS salts supplemented with 0.1 mg/l of IBA. Within five weeks of transfer, there was good rooting in 95% shoot cuttings. Plantlets were transferred to plastic pots maintained within a polythene tent. After two weeks of acclimatization they were transferred to the open environment where 80% of the plantlets survived.

Knowledge Management: Journal