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Study on Isolation and Identification of Salmonella and Escherichia Coli from Different Poultry Feeds of Savar Region of Dhaka, Bangladesh

A. Chowdhuri
Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh

A. Iqbal
Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh

M. Giasuddin
Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh

A. A. Bhuiyan
Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh

Abstract/ Executive Summary:

The study was conducted aiming at the isolation and identification of Salmonella and Escherichia coli (E. coli) from different brands of poultry feeds sold in Savar, Dhaka, Bangladesh. Seven different poultry feeds were subjected to microbiological analysis. All these samples were analyzed by culturing in different media such as nutrient broth (NB), nutrient Agar (NA), SS Agar (Salmonella-Shigella Agar), BGA (brilliant-green Agar), Mac Conkey, DHL and EMB (eosin methylene blue) media. Total bacterial colonies of all the samples were counted separately on the nutrient Agar media. Hence, bacteria were counted to be 9.5×105 in the feed sample C (Layer) which was found to be the highest in number among the poultry feeds. Total viable count (TVC) of Salmonella and E. coli in the feed samples were as 0 to 6.75×104 and 0 to 3.05×104 respectively. Both organisms were found in 71.43% and 57.14% of the analyzed feed samples, respectively. The highest number of Salmonella was found in sample C (Layer) feeds and that of E. coli was found in sample B (Grower) feeds. The widespread occurrence of Salmonella and E. coli in poultry feeds reinforces the need for effective control measures, hygiene in processing and handling of feeds.

Keywords: Salmonella; Escherichia coli; Poultry feeds; Total viable count; Contamination; Hygiene.

Location: Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka, Bangladesh

Start Date: 00-04-2010

End Date: 00-06-2010

Program Area: Animal Health and Management

Commodity: Feed

Objectives:

The objectives of the present study is to investigate (i) the isolation and identification E. coli and Salmonella strains from different sources of poultry feeds, (ii) the prevalence and enumeration of E. coli and Salmonella from different sources of poultry feeds and (iii) the causes of contamination of bacterial load in the poultry feeds.

Methodology:

Study area: The present study was carried out at the Animal Health Research Division (AHRD), BLRI, Savar, Dhaka during the period from April 2010 to June 2010. Collection of samples: Seven different brand types of poultry feed samples were aseptically collected from the different poultry farm and poultry market places at Savar, Dhaka, Bangladesh. These samples of different brands were labeled by Sample A (Grower), Sample B (Grower), Sample C (Layer), Sample D (Starter), Sample E (Starter), Sample F (Layer), Sample G (Starter) and taken immediately to the laboratory of BLRI, Savar, Dhaka. Bacteriological analysis: The samples were analyzed within 2-6 hours of collection. The different media such as nutrient Agar (NA), nutrient Broth (NB), SS Agar (Salmonella-Shigella Agar), BGA (Brilliant Green Agar), EMB (eosin methylene blue), Mc Conkey and DHL were prepared separately. The last five media are called selective media. The above media were prepared separately.

Source of Information: J. Sci. Res. 3 (2), 403-411 (2011)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The presence of the above bacteria in all the feed samples calls for attention in the storage strategies employed by the poultry feed manufacturers, the ware house condition, distributors and the sellers. The absence of Salmonella and E. coli from other poultry feeds suggests that the food processing is well handled. In recent years, with the increasing in density of poultry and infectious diseases in poultry caused by pathogenic bacteria, the healthy development of the poultry industry is facing serious threat. These result in lower feed conversion rates, the declining in egg laying rates of hens as well as fertilization rates, slower growth, the increasing rejection rates of dead chicken and even a large number of deaths. Poor management of farming, overcrowding, dirty sanitation environment, bad ventilation, poor feed quality and stress can cause chicken to infect with diseases. Therefore, how to take effective measures to prevent and control infectious diseases from chicken is the most important task.

Knowledge Management: Journal