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In Vitro Tuberization of Potato Influenced by Benzyl Adenine and Chloro Choline Chloride

M. ZAKARIA
Asstt. Professor
Deptt. of Horticulture, BSMRAU, Gazipur.

M. M. HOSSAIN
Professor
Deptt. of Horticulture, BSMRAU, Gazipur.

M. A. KHALEQUE MIAN
Professor
Deptt. of Genetics and Plant Breeding, BSMRAU, Gazipur.

T. HOSSAIN
Professor
Deptt. of Crop Botany, BSMRAU, Gazipur.

M. Z. UDDIN
Senior Scientific Officer
HRC, BARI, Gazipur-1701, Bangladesh.

Abstract/ Executive Summary:

In an experiment, six levels of benzyl adenine (0.2.5,5.0,7.5,lO.0,12.5 and 15.0 mg/I) in combination with three levels of (CCC) chloro choline chloride (0,125,250 and 500 mg/I) were evaluated against control treatment (0) in each case to find out their optimum levels for microtuberization in potato variety Diarnant. Microtuberization was the earliest by I 3 days at 10 mg/I BA. The number and average weight of microtuber per flask vas increased with increasing rate of BA and reached maximum of 12.9 and 252.1 mg respectively at 10 mg/I BA and decreased onwards with further increase of BA concentration. The increase rate of CCC increased the number of microtuber but decreased average weight. The maximum number and average weight of microtuber were recorded at 500 mg/I CCC and in absence of CCC respectively.

Keywords: BA, CCC, Potato, Microtuber.

Location: Deptt. of Horticulture, BSMRAU, Gazipur.

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Potato

Objectives:

To determine the optimum level ol BA and CCC for getting higher proportion of large size microtubers of Diamant.

Methodology:

In vitro ready stocks of plantlets of potato variety Diamant were used and the number of plantlets was increased by subculturing via nodal cutting at 3 weeks interval. The multiplication medium contained mineral salts and vitamins plus 0.1 mg/I GA₃, 0.01 mg/I NAA, 4 mg/I calcium pantathonate and 30g/l sucrose. The medium was solidified with 8 g/l agar. Temperature in the growth chamber was 23 ± 1°C with 16 hours photoperiod and light was supplied by fluorescent tubes at an intensity of 3000 lux. Eight stem segments (each with 3 nodes) of subcultured in vitro plantlets were cultured in 250 ml Erlenmeyer flasks containing 40 ml microtuber induction medium, which was based on MS medium supplemented with 80 g/l sucrose. Six levels of Benzyl adenine (BA) and three levels Chloro choline chloride (CCC) having control treatment (0) in each case were added in the culture medium, which in combination made 28 treatment combinations. The experiment was laid out in a Completely Randomized Design (CRD) with four replications. The number of flask per replication was 72. The induced microtubers were harvested aseptically after 70 days of incubation period. The collected data were analyzed with the help of computer using MSTAT-5 program and the mean separation was done by Duncan’s new multiple range test.

Source of Information: ISSN 0258-7122, Bangladesh J. Agril. Res. 33(3) : 419-425, September 2008

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The number and average weight of microtuber per flask vas increased with increasing rate of BA and reached maximum of 12.9 and 252.1 mg respectively at 10 mg/I BA and decreased onwards with further increase of BA concentration. The increase rate of CCC increased the number of microtuber but decreased average weight. The maximum number and average weight of microtuber were recorded at 500 mg/I CCC and in absence of CCC respectively.

Knowledge Management: Journal