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Culture of Rotifer Brachionus Angularis Hauer Feeding with Dried Chlorella

Shabnam Mostary
Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh

M. Shahidur Rahman
Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh

M. Amzad Hossain
Department of Fisheries, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur

Abstract/ Executive Summary:

A study was carried out to know the performance of powdered dried Chlorella for culture of rotifer Brachionus angularis. B. angularis was fed with powdered dried Chlorella in treatment 1, live or fresh cultured Chlorella in treatment 2 and baker’s yeast in treatment 3. The rage of physicochemical parameters during culture of rotifer such as water temperature, air temperature, pH and dissolved oxygen were within the suitable ranges for B. angularis culture and more or less similar in all the treatments. The highest population densities of B. angularis recorded in treatment 1, treatment 2 and treatment 3 were 50, 60 and 30 ind/ml, respectively. The mean population densities were 30.1±12.2, 37.4±14.6 and 21.1±6.1 ind./ml in treatment 1, treatment 2 and treatment 3, respectively. The results revealed that the powdered dried Chlorella had significant effect on the population density of B. angularis and was better than that of baker’s yeast. So, when live Chlorella will not be available, powdered dried Chlorella can be successfully used as feed for B. angularis culture.

Keywords: Rotifer, Chlorella, yeast, culture medium

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Program Area: Knowledge Management

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Objectives:

The present study was undertaken to culture rotifer B. angularis using powdered dried Chlorella.

Methodology:

Inexpensive organic culture medium was prepared with 700 g of pulse bran (Vigna mungu) mixed in 20 L tap water in a plastic bucket. Then, after 1 week, 11 g urea was added to each bucket. After 4 weeks partially decomposed pulse bran mixture was filtered through thin mark in cloth and solid materials were discarded. The supernatant was siphoned to another bucket after another week and 2 g lime (CaO) per liter of medium was mixed to make it clear and pH was adjusted to 7 adding H₂SO₄. Then, after a week the clear supernatant was again siphoned to another bucket and this clear solution was ready as the algal culture medium. The prepared medium was sterilized in an autoclave (at 121⁰C) for 15-20 minutes Rotifer (B. angularis) were cultured in 3 L size plastic jars containing 1 L water. All the jars were stocked with B. angularis (from stock rotifer culture) at the initial density of 10 rotifer/ml. The experiment was conducted with three treatments each treatment with 3 replications. Powder dried Chlorella (0.1 g/L water), fresh Chlorella (1.2x106 cells/ml water) and baker’s yeast (0.15 g/L water) were used as feed for rotifer in treatment 1, treatment 2 and treatment 3, respectively. All the jars were supplied with continuous aeration for 24 hours to maintain adequate dissolved oxygen level. Continuous lighting for 24 hours was arranged for better growth of B. angularis. Water temperature, air temperature, pH, DO, etc. were monitored regularly. Density of B. angularis was determined daily with a zooplankton counting cell under a compound microscope. Statistical analysis: Data were subjected to analysis of variance (ANOVA) and Duncan’s Multiple Range Test (DMRT) for significance in variance

Source of Information: Univ. j. zool. Rajshahi Univ. Vol. 26, 2007. pp. 73-76 ISSN 1023-6104

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Achievement/Findings:

The mean value of B. angularis density fed on fresh cultured Chlorella under treatment 2 was 37.44±14.6 ind./ml. The mean value of B. angularis fed on baker’s yeast under treatment 3 of the present experiment was the lowest, 21.11±6.11 ind./ml. It is reflected from the present study that the mean population density of B. angularis was the highest under treatment 2 that was fed on fresh cultured Chlorella. The mean population density of B. angularis was higher under treatment 1 that fed on powdered dried Chlorella under treatment 1 feed on baker’s yeast and was close to treatment 2. This indicated that fresh cultured or live Chlorella were the best as food for rotifer B. angularis and dried powdered Chlorella were also better than baker’s yeast as food for the rotifer. One of the reasons for the slightly lower production of B. angularis fed on powdered dried Chlorella under treatment 1 than that fed on fresh cultured live Chlorella under treatment 2 was the acclimation of rotifer with live Chlorella as feed in the stock culture from where the rotifer were taken into the experimental jars. Another reason is that when dried, algal digestibility probably would increase but the nutritional quality may decrease. It may be concluded that, as the powdered dried Chlorella is better than baker’s yeast as feed and it has significant effect on the population density of B. angularis, so, when live Chlorella will not be available, powdered dried Chlorella can be successfully used as a feed for B. angularis during its culture.

Knowledge Management: Journal