Agricultural Research Management Information System

  • Home
  • Research Summary
    • All
    • Government Organization
      • Agriculture Training Institute, Ishwardi, Pabna
      • Bangabandhu academy for poverty alleviation and rural development (BAPARD)
      • Bangabandhu Sheikh Mujibur Rahman Science & Technology University
      • Bangladesh Bureau of Statistics
      • Bangladesh Institute of Health Sciences
      • Bangladesh Institute of Tropical & Infections Diseases (BITID)
      • Bangladesh Meteorological Department
      • Bangladesh National Herbarium
      • Bangladesh Space Research and Remote Sensing Organization
      • Bangladesh Technical Educational Board
      • Barind Multipurpose Development Authority
      • Central Cattle Breeding Station
      • Department of Agriculture Extension
      • Department of Fisheries
      • Department of Livestock Services
      • Department of Youth Development
      • Dhaka Medical College
      • Geological Survey of Bangladesh
      • Institute of Epidemiology, Disease Control & Research
      • Jatiya Kabi Kazi Nazrul Islam University
      • Khulna Govt. Women College
      • Livestock Training Institute
      • Local Government Engineering Department
      • Ministry of Agriculture
      • Ministry of Environment and forest
      • Ministry of Fisheries and Livestock
      • Ministry of Labour & Employement
      • Ministry of Land
      • Ministry of Public Administration
      • Ministry of Textiles and Jute
      • Ministry of Water Resources
      • Ministry of Youth and Sports
      • National Agricultural Training Academy
      • National institute of preventive and social medicine
      • National Mushroom Development and Extension Centre
      • Pabna University of Science and Technology
      • Seed Certification Agency
      • Shaheed Suhrawardy Medical College
      • Sheikh Hasina University
      • University Grants Commission
      • Youth Training Centre
    • Autonomous/Semi-gov Org
      • Bangladesh Academy for Rural Development
      • Bangladesh Agricultural Development Corporation
      • Bangladesh Atomic Energy Commission
      • Bangladesh Council of Scientific and Industrial Research
      • Bangladesh Fisheries Development Corporation
      • Bangladesh Institute of Development Studies
      • Bangladesh Institute of Management
      • Bangladesh Milk Producers Cooperative Union Limited
      • Bangladesh Water Development Board
      • BIRDEM
      • Center for Environmental and Geographic Information Services
      • Hortex Foundation
      • Institute of Water Modeling
      • National Institute of Biotechnology
      • River Research Institute
      • Rural Development Academy
    • NARS
      • Bangladesh Agricultural Research Council
      • Bangladesh Agricultural Research Institute
      • Bangladesh Fisheries Research Institute
      • Bangladesh Forest Research Institute
      • Bangladesh Institute of Nuclear Agriculture
      • Bangladesh Jute Research Institute
      • Bangladesh Livestock Research Institute
      • Bangladesh Rice Research Institute
      • Bangladesh Sericulture Research and Training Institute
      • Bangladesh Sugarcrop Research Institute
      • Bangladesh Tea Research Institute
      • Bangladesh Wheat and Maize Research Institute
      • Cotton Development Board
      • Soil Resource Development Institute
    • Public University
      • Ahsanullah University of Science and Technology
      • Bangabandhu Sheikh Mujibur Rahman Agricultural University
      • Bangamata Sheikh Fojilatunnesa Mujib Science and Technology University
      • Bangladesh Agricultural University
      • Bangladesh Open University
      • Bangladesh University of Engineering and Technology
      • Bangladesh University of Professionals
      • Bangladesh University of Textiles
      • Barisal Government Veterinary College
      • Begum Rokeya University
      • Chittagong University of Engineering and Technology
      • Chittagong Veterinary and Animal Science University
      • Comilla University
      • Dhaka University of Engineering & Technology
      • Dinajpur Government Veterinary College, Dinajpur
      • Gono Bishwabidyalay
      • Hajee Mohammad Danesh Science and Technology University
      • Islamic University, Kushtia
      • Jagannath University
      • Jahangirnagar University
      • Jessore University of Science and Technology
      • Jhenaidha Government Veterinary College
      • Khulna Agricultural University
      • Khulna University
      • Khulna University of Engineering & Technology
      • Mawlana Bhashani Science and Technology University
      • Millitary Institute of Science and Technology
      • National University
      • Noakhali Science and Technology University
      • Patuakhali Science and Technology University
      • Rajshahi University of Engineering and Technology
      • Shahjalal University of Science & Technology
      • Sher-e-Bangla Agricultural University
      • Sylhet Agricultural University
      • Sylhet Government Veterinary College
      • University of Barisal
      • University of Chittagong
      • University of Dhaka
      • University of Rajshahi
    • Private University
      • Asian University of Bangladesh
      • Atish Dipankar University of Science and Technology
      • BGC Trust University Bangladesh
      • BGMEA University of Fashion & Technology (BUFT)
      • BRAC University
      • City University
      • Daffodil International University
      • East West University
      • Exim Bank Agricultural University
      • Gana Bishwabiddalaya
      • Hamdard University
      • Independent University, Bangladesh
      • International Islamic University Chittagong
      • International University of Business Agriculture and Technology
      • Islamic University of Technology
      • Leading University, Sylhet
      • North South University
      • Premier University
      • Primeasia University
      • Private University
      • SOAS, University of London
      • Southeast University
      • Stamford University
      • State University of Bangladesh
      • The Millenium University
      • University of Asia Pacific
      • University of Development Alternative
      • University of Information Technology and Sciences
      • University of Liberal Arts Bangladesh
      • University of Science and Technology, Chittagong
      • World University
    • INGO/IO/NGO/Private Org
      • ACI Limited
      • Agricultural Advisory Society (AAS)
      • Apex Organic Industries Limited
      • Arannayk Foundation
      • Bangladesh Academy of Sciences
      • Bangladesh Centre for Advanced Studies
      • Bangladesh Institute of Social Research
      • Bangladesh Science Foundation
      • Bangladesh Unnayan Parishad
      • BAPA
      • BRAC
      • CARE Bangladesh
      • CARITAS
      • Centre for Environmental Geographical Information System
      • Centre for Policy Dialogue (CPD)
      • Creative Conservation Alliance
      • Dhaka Ahsania Mission
      • Dwip Unnayan Sangstha
      • EMBASSY OF DENMARK, BANGLADESH
      • Energypac Limited Bangladesh
      • FAO- Bangladesh
      • FIVDB
      • ICDDRB, Mohakhali, Dhaka-1212
      • iDE Bangladesh
      • Innovision Consulting Private Ltd.
      • International Center for Climate Change and Development
      • International Centre for Integrated Mountain Development
      • International Development Research Centre
      • International Fertilizer Development Center, Bangladesh
      • International Food Policy Research Institute
      • International Maize and Wheat Improvement Centre
      • International Potato Center
      • IRRI- Bangladesh
      • IRRI-Philippines
      • Ispahani Agro LTD
      • IUCN, Bangladesh
      • Krishi Gobeshina Foundation
      • Lal Teer
      • Mennonite Central Committee
      • Metal (Pvt.) Ltd
      • Modern Herbal Group
      • Palli Karma-Sahayak Foundation
      • Practical Action Bangladesh
      • Proshika
      • RDRS Bangladesh
      • RIRI-Philippines
      • Rothamsted Research
      • SAARC Agricultural Centre
      • SAARC Meteorological Research Centre
      • Social Upliftment Society
      • South Asia Enterprise Development Facility
      • Square Pharmaceuticals Ltd.
      • Supreme Seed
      • Transparency International Bangladesh
      • Unnayan Onneshan
      • USAID
      • Water Resources Planning Organization
      • Winrock International
      • World Bank
      • World Food Program
      • World Vegetable Center
      • WorldFish Centre, Bangladesh
    • Foreign University
      • Asian Institute of Technology
      • Auckland University of Technology
      • Australian National University
      • Bidhan Chandra Krishi Viswavidyalaya
      • BOKU-University of Natural Resources and Applied Life Sciences
      • Cranfield University
      • Curtin University
      • Foreign University/ Institute
      • Hiroshima University
      • Hokkaido University
      • Huazhong Agricultural University
      • International Islamic University, Malaysia
      • Kagawa University
      • Kangwon National University
      • Kochi University
      • Kyoto University
      • Kyushu University
      • Ladoke Akintola University of Technology
      • Murdoch University
      • Nagoya University
      • NOAA-CREST, CCNY
      • Royal Veterinary and Agricultural University
      • San Diego State University
      • Shinshu University
      • Tottori University
      • United Nations University
      • University Malaysia Kelantan
      • University Malaysia Pahang
      • University Nova de Lisboa
      • University of Alberta
      • University of Bremen
      • University of Bremen
      • University of Calgary
      • University of california
      • University of Greenwich
      • University of Hamburg, Hamburg
      • University of Hannover
      • University of Hawaii
      • University of Helsinki, Finland
      • University of Kalyani
      • University of Leeds
      • University of Liverpool
      • University of Malaya
      • University of Milan
      • University of New England
      • University of Philippines
      • University of Plymouth
      • University of Queensland
      • University of Reading
      • University of Southampton
      • University of Texas
      • University of the Punjab
      • University of Tokyo
      • University of Toronto
      • University of Wales
      • University of Washington
      • University of Wollongong
      • University Putra Malaysia
      • University Sains Malaysia
  • Search
    • Search by Keyword
    • Search by Organization
    • Search by Program Area
    • Search by Commodity/Non-commodity
    • Search by Funding Source
    • Search by Researcher
    • Custom Search
    • On-going Research
  • About Us
    • ARMIS
    • Brochure
  • Contact Us
    • BARC Personnel
    • ARMIS Personnel
    • Feedback
  • Report
    • All
    • By Organization
      • Bangladesh Agricultural Research Council
      • Bangladesh Agricultural Research Institute
      • Bangladesh Fisheries Research Institute
      • Bangladesh Forest Research Institute
      • Bangladesh Institute of Nuclear Agriculture
      • Bangladesh Jute Research Institute
      • Bangladesh Livestock Research Institute
      • Bangladesh Rice Research Institute
      • Bangladesh Sericulture Research and Training Institute
      • Bangladesh Sugarcrop Research Institute
      • Bangladesh Tea Research Institute
      • Bangladesh Wheat and Maize Research Institute
      • Cotton Development Board
      • Soil Resource Development Institute
    • Research Trend Analysis
  • User Request
  • Data Input
  • Help
    • Operation Manual
      • PDF
      • Video
    • Program Area & Commodity
  • We have reached 37600 number of research entries at this moment.
    • Logout

Research Detail

  1. Home
  2. Research
  3. Detail
M. M. ALI
Oilseed Research Centre, BARI

M.A. KHALEQUE MIAN
Department of Genetics and Plant Breeding, BSMRAU, Bangladesh

J.B.M. CUSTERS
Plant Research International, The Netherlands.

M.M.H. KHURRAM
Oilseed Research Centre, BARI

The experiment was conducted at the Tissue Culture Laboratory as well as in the net house of Oilseed Research Centre, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur on microspore culture and the performance of microspore derived doubled haploid (DH) in Brassica juncea (L.). The variety BARI Sarisha II of B. junccea was used for microspore culture and 8 DH genotypes viz., BJDH 01, BJDH 05, BJDH ii, BJDH 12, BJDH 14, BJDH 17, BJDH 18, and BJDH 20 along with their parent Rajat collected from the Plant Research International, the Netherlands were used for performance evaluation. Four-day cold treatment at 6°C along with bud size 3.0-3.1 mm of BARI Sarisha II showed better response in the embryonic development. Significant variation was observed for the different yield contributing characters among the DH genotypes along with their parent Rajat. All the DH genotypes were late both for days to flower and days to maturity compared to the parent. Most of the DH genotypes produced higher seed yield than the parent. The genotypes BJDII 14 (7 g) and BJDH 01(7 g) were bold seeded and almost double or more in 1000-seed weight compared to the parent. Total oil content of the DH genotypes (40.64-42.28) were significantly different from the parent (3 g). Oil content of BJDH 01 was almost 3% higher than the parent could be selected to incorporate in the future breeding program.

  Microspore culture, Doubled haploid, Brassica juncea.
  Tissue Culture Laboratory and the net house of Oilseed Research Centre, Bangladesh Agricultural Research Institute
  
  
  Variety and Species
  Mustard

The present research work was undertaken to develop a suitable protocol for microspore embryogenesis in B. juncea and to comparative study between amphidiploid Rajat and its microspore derived DH generation for different agronomic traits.

The experiment was conducted in the Tissue Culture Laboratory and the net house of Oilseed Research Centre, Bangladesh Agricultural Research Institute. The genotypes BARI Sarisha 11 of B. juncea was used for microspore embryogenesis, while the DH genotypes collected from Business unit, Plant Research International, The Netherlands under the project International Co-operation for the Developing Countries (FNCO-DC) were used for performance evaluation. a) Microspore culture: For microspore culture, inflorescences of BARI Sarisha 11 having 1-3 buds flowering were collected from the field and exposed to 1-6 days at 6°C in refrigerator taking in a beaker with water. For microspore isolation 6-8 inflorescences were taken from the beaker. Desired buds (mostly 2.8- 3.2 mm) were picked with a fine tweezers and classified in 3 lengths group by using a measuring gauge. Ten buds per length group were selected. The laminar flow hood was switched on 30 minutes in advance and disinfected with 70% Ethyl alcohol (EtOH). Hands were also sterilized with EtOH. The buds were transferred in to tea-sieves for sterilization 10 mm in 2% NaOC1 making sure there were no air bubbles left. The buds were rinsed in sterile tap water for 1, 4 and 10 minutes. Adding 2 ml of sterile NLN 13 medium in 50 ml beaker, the buds were homogenized with injection piston by a turning pressure movement. Rinsing the piston on the 50 ml beaker, the suspension was poured over the sterile filter funnel with 10 ml centrifuge tubes. The volume of all the tubes adjusted upto 10 ml. The suspensions with microspores were centrifuged 3 mm at 800 rpm in 4°C adjusted. Supernatant was poured off and resuspended the pellet with loml medium. Centrifugation and resuspension were repeated twice. After the last washing,, microspores were resuspended in I ml NLN 13. Taking a sample of 15 μl microspores suspensions were counted by Fuchs Rosenthal counting chamber. The number (X) of microspores in 5 sub cells multiplied by 16000 were the density of microspore per ml. NLN 13 medium was added in the tube until the right density for cultivation (4x104 microspore/mi). The petridishes(TC quality) of 3 cm in diameter (for I ml culture) were filled out and tapped with Paraffin. The cultures were placed 35°C for 2 days and then transferred to 25°C continuously in dark. The embryo development was observed by an inverted microscope regularly. After 14 days globular, embryo development was found. Finally, after 21 days, the embryos were counted. b) Performance evaluation of DH of B. juncea: The seeds of eight DH genotypes viz., BJDH 01, BJDH 05, BJDH 11, BJDH 12, BJDH 14, BJDH 17, BJDH 18, BJDH 20 and along with the parent Rajat were sown in the pots (8? × 12?) on November 2004. The pots were arranged in a randomized block design with 3 replications. Ten seeds were sown in each pot. The seedlings emerged three to four days after sowing. After 15 days only, 4 plants were allowed to grow well in each pot. The data days to flower, days to maturity, plant height, primary branches per plant, secondary branches per plant, siliquae per plant, siliqua length, seeds per siiiqua, 1000-seed weight, yield per plant were recorded from selected four plants. Total oil content and fatty acid composition were estimated using by Soxhelt’s extraction apparatus and Gas Liquid Chromatograph (GLC), respectively. Analysis of variance and mean separation was done by Duncan’s Multiple Range test (DMRT) following the LStat program.

  Bangladesh J. Agril. Res. 33(3) : 571-578, December 2008 ISSN 0258-7122
  
Funding Source:
  

There was a significant variation observed for the character oil content among the DII genotypes along with their parent Rajat . Total oil content of the DH genotype BJDH 01(42.28%) was the maximum followed by the genotype BJDH 14 (41.62%) and were statistically different from the remaining genotypes along with the parent, which contained 39.49%. Oil content of the DH genotypes BJDH 12, BJDH 17 and BJDH 18 were similar. A range of variation was observed for fatty acid composition in oil of DH genotypes along with their parent. The variation for palmitic (C16 2.33 - 3.69%) acid content for all the genotypes along with parent was low. On the contrary, the ranges of other fatty acids C18:1 (5.83-10.36%), C18:2 (15.3222.28%) C18:3 (13.3120.16%), C201 (4.52-11.93%), and C22.1 (41 .89-53.57%) were found high. Though the erucic acid content was high in all the DH genotypes including parent, minimum of 41.89% was found in the parent Rajat, while the maximum in the DH genotype BJDH 18 of 53.57% followed by BJDH 05 of 53.07%. The DH genotype BJDH 01 could be exploited in future breeding programme for its higher oil content in the seed.

  Journal
  


Copyright © 2025. Bangladesh Agricultural Research Council.