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Various Color Illumination Effect on in Vitro Multiple Shoot Induction in Water Chestnut (trapa Japonica)

A. Hoque
Department of Agronomy and Agricultural Extension, University of Rajshahi, Rajshahi-6205, Bangladesh.

S. Arima
Faculty of Agriculture, Saga University, Saga-840, Japan

Abstract/ Executive Summary:

Shoot production efficiency through cotyledonary nodes as well as nodal explants were studied under various color illumination conditions in water chestnut (Trapa japonica Flerov). Under various color (white, blue, mixed, red and intra-red) illuminations, mixed color illumination showed the early and higher percentage of shoot proliferation. Fresh and dry weight were also significantly higher under the mixed color condition. Media consisted of MSMA with 2.7 μM BA, 0.5 μM NAA and 0.5 μM GA3 in all color illuminations. The competence of cotyledonary node as well as nodal explants for the induction of multiple shoots under the influence of various color illumination as well as plant growth regulators have been observed. Shoots produced in vitro rooted 100% in liquid MS supplemented with 5.4 μM IBA. Plantlets were established successfully in soil.

Keywords: Color illumination, Multiple shoot, Induction, Water chestnut

Location: Department of Agronomy and Agricultural Extension, University of Rajshahi, Rajshahi-6205, Bangladesh.

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Water chestnut

Objectives:

To determine the Various Color Illumination Effect on In vitro Multiple Shoot Induction in Water Chestnut (Trapa japonica)

Methodology:

Mature, green, bi-spinate, small fiuits of water chestnut (Trapa japonica Flerov) were harvested from a water chestnut field at Saga University, Japan and used as the experimental materials. Fruits (50 at a time) were rinsed in distilled water (300 ml) containing three drops of Tween 80 detergent for 15 min. Tween 80 was removed by washing six or seven times in distilled water. The apical end of the fiuit, that partially covers the large cotyledon, plumule, small cotyledon and hypocotyls, was excised (6 - 7 mm3) and the fruit was surface-sterilized in a 3% (w/v) sodium hypochlorite solution for 3 min. After three rinses in sterile deionized distilled water, the pericarp was removed and the white embryonal explants (2 - 3 mm long) were excised and cultured on a semi-solid agar 0.6% (w/v) Katayama Co., Japan. MSMA nutrient media supplemented with 2.7 μM BA, 0.5 μM NAA and 0.5 μM GA3. Within three to five days of culture under various color illumination (viz. white, blue, mixed, red and infra-red) explants formed cotyledonary nodes early under the mixed color condition. In all treatments, organic nutrients viz. myo-inositol 540.0 μM, pyridoxine 5.4 μM and sucrose 3% were used. After two weeks of germination on semi-solid media the explants were transferred to culture tubes (55 mm ∇ 100 mm) with 10 ml liquid of the same constitution. For facilitating elongation of shoots and roots, the volume of the liquid medium in the culture vessels was raised to 30 ml. The rooting of shoots was tested by addition of 5.4 μM IBA to the liquid MS under white fluorescent tubes. Rooted plantlets were thoroughly washed to remove all traces of the medium and transferred to plastic pots (50 cm ∇ 30 cm ∇ 22 cm H) filled with sterilized garden soil up to a depth of 5 cm topped with water up to 15 cm. The pots (50 cm ∇ 30 cm ∇ 22 cm H) were kept in the net house under sunlight. The media were adjusted to pH 5.8 prior to autoclaving. Cultures were maintained at 28 ± 1°C under a 14 h photoperiod with various color provided by color illumination system. Each experiment was randomized completely, with three replicates per treatment and 18 explants per replicate. Observations were recorded at three weeks interval for shoot multiplication, fresh and dry weight and rooting. The mean values were statistically compared using the Duncan (1955) multiple range test at p < 0.05.

Source of Information: Plant Tissue Cult. 14(2) : 161-166, 2004 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The competence of cotyledonary node as well as nodal explants for the induction of multiple shoots under the influence of various color illumination as well as plant growth regulators have been observed. Shoots produced in vitro rooted 100% in liquid MS supplemented with 5.4 μM IBA. Plantlets were established successfully in soil. The results in this paper under various color illumination regimes showed that the mixed color was the best for early and higher multiple shoot proliferation producing significantly higher fresh and dry weight of the tissue culture derived material.

Knowledge Management: Journal