Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Micropropagation of Aloe Barbadensis Mill. Through in Vitro Culture of Shoot Tip Explants

R. Baksha
Biotechnology Division, BRRI, Gazipur-1701, Bangladesh.

Miskat Ara Akhter Jahan
Biological Research Division, Dhaka Laboratories, BCSIR, Dhaka-1205, Bangladesh

Rahima Khatun
Biological Research Division, Dhaka Laboratories, BCSIR, Dhaka-1205, Bangladesh

John Liton Munshi
Biological Research Division, Dhaka Laboratories, BCSIR, Dhaka-1205, Bangladesh

Abstract/ Executive Summary:

Multiple shoots (ten per explant) in Aloe barbadensis were obtained from shoot tip explant cultured on MS supplemented with BAP (2.0 mg/l) and NAA (0.5 mg/1). About 95% rooting was obtained from micro-shoots cultured on half strength MS supplemented with NAA (0.5 mg/1). Well-developed rooted plantlets were successfully transferred to the soil with 70% survival.

Keywords: Aloe barbadensis, Medicinal plant, Shoot tip, Micropropagation

Location: Biological Research Division in Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh

Start Date: 00-00-2003

End Date: 00-00-2004

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

To identify the Micropropagation of Aloe barbadensis Mill. Through In vitro Culture of Shoot tip Explants

Methodology:

The experiment was conducted at Biological Research Division in Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh, during 2003 - 2004. Young shoot tips of the suckers were collected from the field of BCSIR, Dhaka, and used as primary explants. Explants were washed under running tap water for ten to 15 min and then surface-sterilized with 0.1% HgCl₂ for 10 min followed by five to six rinses with sterilized distilled water. The outer leaves were discarded and inner shoot apices were inoculated either in 25 mm × 150 mm culture tubes or 100 ml/250 ml Erlenmeyer flasks containing MS supplemented with various combinations and concentrations of auxins and cytokinins. Before inoculation, the media were gelled with agar (0.6%), pH was adjusted to 5.8 and autoclaved at 121⁰C for 15 min. The flasks containing explants were then incubated at 25 ± 0.5⁰C, under fluorescent tube light with 16 h photoperiod. Subcultures were made at three - four weeks interval. Results were recorded for each culture before subculture. The number of replications was 25 for each experiment with three replications. From each subculture, microshoots (more than 1.0 cm long) were separated out and implanted in the rooting medium containing half strength MS with various concentrations and combinations of auxins for root induction. For hardening, the culture tubes containing rooted shoots were kept at normal room temperature and light for 14 days. Thereafter, the rooted shoots were removed from culture tubes, washed thoroughly to free agar from roots and transplanted to small pots containing soil and sand (2 : 1 v/v). Plantlets were well-covered with a piece of polythene sheet for three weeks to ensure high humidity while watering was continued regularly. After three months the plantlets were transferred to the open field.

Source of Information: Plant Tissue Cult. & Biotech. 15(2): 121-126, 2005 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The present findings are in agreement with those observed in other plant species such as Asparagus sp. This might be due to genotypic variation of explants reinforced by the cultural and environmental conditions. About 95% rooting was obtained from micro-shoots cultured on half strength MS supplemented with NAA (0.5 mg/1). Well-developed rooted plantlets were successfully transferred to the soil with 70% survival.

Knowledge Management: Journal