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Efficacy of Ipm Biopesticide in Controlling Foot/collar Rot of Eggplant Caused by Sclerotium Rolfsii

M. S. Islam
IPM Lab, Department of Plant Pathology, Bangladesh Agicultural University, Mymensingh, Bangladesh

M. B. Meah
1PM Lab, Department of Plant Pathology, Bangladesh Agicultural University, Mymensingh, Bangladesh

M. I. Khalil
Plant Pathology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh

A. T. M. S. Islam
Departmcnt of Plant Pathology, HSTU, Dinajpur, Bangladesh

Abstract/ Executive Summary:

The effect of lPM biopesticide and Trichoderma suspension in reducing foot/collar rot of a susceptible eggplant var. Dhohazari was studied in net house and field condition. In net house experiment, both the Trichoderma formulation and Trichoderma suspension treatment produced the lowest plant infection compared with inoculated control. But incase of percent plant recovery, Trichoderma formulation showed the better performance over Trichoderma suspension. In field condition, formulated Trichoderma needs 6-9 days of incubation period to act as an antagonist applied before inoculation. However , for complete inhibition of the pathogen 9 days of incubation is required.

Keywords: Eggplant , Incubation period , Sclerotium rolfsii , Trichoderma

Location: Bangladesh Agricultural University , Mymensingh

Start Date: 00-05-2007

End Date: 00-07-2007

Program Area: Pest Management

Commodity: Brinjal, Diseases

Objectives:

To determine the efficacy of Trichoderma based biopesticide and the incubation period for Trichoderma to act as an antagonist against Sclerotium rolfsii.

Methodology:

Net house experiment: The experiment was conducted in the net house of the Department of Plant Pathology, Bangladesh Agricultural University (BAU), Mymensingh during May to July 2007 . The IPM Biopesticide (formulated) and the bioagent Trichoderma harzianum CP were collected from IPM laboratory, Bangladesh Agricultural University, Mymensingh. The experiment was conducted following completely randomized design with four replications. Seedlings were raised in tray (40cm x 30 cm). Tray soil was prepared by mixing soil, sand and well decomposed cowdung in the proportion of 2: I: 1 and sterilized with formalin (Dasgupta, 1988). Collar rot susceptible cultivars Dohazari G were used in the study. Trichoderma suspension was prepared by using 7 days old pure culture of Trichoderma harzianum on Potato Dextrose Agar and distilled water. Thirty gram IPM , biopesticide @ 0.6% (w/w) and 200m! Trichoderma suspension (5 x 106 conidia ml^-1 ) were applied per tray. The virulent isolate of Sclerotium rolfsii was isolated from naturally infected eggplant and grown on barely grains were used as inocula. Inoculations were done seven days after antagonist used . Ten gram of barley culture was used per tray. Data were recorded on number of plants infected, number of plants non-infected and number of plants recovered from infection in different ages in different treatments. Field experiment: The experiment was conducted in the field laboratoty of the Department of Plant Pathology, BAU, Mymensingh during September 2007 to April 2008. Collar rot susceptible cultivars Dohazari G were used in the study and seedlings were raised in tray.Twelve healthy seedlings of 30 days were transplanted in each plot following randomized complete block design with three replications maintaining plant to plant distance 50 em and line to line 1 meter.Irrigation and weeding were done as and when necessary. Following the application of biopesticidc (20 g plan-1 ) ten gyam barley culture of S rolfii was applied to each of those plants first immediately after and then at 24 h interval for 14 days. For the determination of the effect of Trichoderma based biopesticide data were recorded on number of plants infected, number of plants killed and number of plants recovered from infection . Data collected during experimental period were tabulated and analyzed following statistical package MSTAT.

Source of Information: Bangladesh J. Nuclear Agric. 23 & 24: 107-113, 2012; ISSN 0258-7130

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

From the study results , it could be concluded that Trichoderma based biopesticide could be used as a protective measure against foot/collar rot diseases for susceptible varieties of eggplant.

Knowledge Management: Journal