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In Vitro Microtuberisation in Potato Obtained from Diverse Sources

M. J. Hossain
Tuber Crops Research Centre, Bangladesh Agricultural Research Institute, Joydebpur,Gazipur-1701, Bangladesh

Abstract/ Executive Summary:

Seventeen potato cultivars of six diverse sources have been evaluated for their response to in vitro microtuberisation in presence or absence of light. Tuber initiation appeared first in the light and gave higher number of microtubers with higher total as well as mean weight than the dark. Among the cultivars, on the average, Patrones performed best for most of the parameters such as, days to tuber initiation, number of tubers, their weight per plant and mean tuber weight. In terms of microtuber production potential, Chamak was the poorest. Among the different sources, the Dutch and Canadian cultivars showed comparatively better performance in respect of microtuber production potential than the others. The local high yielding varieties were the least productive. The weight of microtubers per tube (534.6 mg) and mean tuber weight (104.3 mg) were also significantly higher in the light compared to dark (264 mg/tube and 87.8 mg/tuber, respectively). Microtuberisation potential is a variety-dependent character, which is largely influenced by environmental factor.

Keywords: Microtuberisation, Potato, Cultivars

Location: Tuber Crops Research Centre, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Potato

Objectives:

To investigating microtuberisation over a wide range of potato sources.

Methodology:

Seventeen potato cultivars from six diverse sources such as, the Dutch cultivars, Canadian cultivars, local high yielding varieties, namely Heera, Dheera and Chamak, indigenous cultivars, promising germplasm lines (P-315 and P-430) and true potato parental lines (TPS-25, TPS-2, TPS-67 and TPS-13) were used in this experiment. The cvs. Atlantic and Kennebec; and TPS parental lines were received from Feredicton Research Station, Canada and International Potato Centre, Lima, Peru, respectively as in vitro microplants. The in vitro microplants of the other materials have been developed in the TCRC's laboratory following a meristem culture method. All the materials were subcultured at 20 days interval via nodal cuttings as described. The cultures were incubated at 3 klux florescent light intensity for 16 h daily and at 22 ± 2°C. Organic salts of MS supplemented with 5 mg/l BAP, 500 mg/l CCC and 8% sucrose were used. The pH of the media was adjusted to 5.8 before autoclaving. Tuberisation media (10 ml) were poured into each of 150 mm × 25 mm tube containing three well developed and rooted microplants. The cultures were incubated at 18 ± 2°C for a period of eight weeks. Fifty per cent cultures were incubated at total darkness and the rest 50% at 16 h 3 klux florescence light daily. The experiment was laid in a two factors (cultivar × light/dark) complete randomised design (CRD) with four replications. Mean of sources were taken from cultivars which were again analysed as sources × light/dark factors following a CRD. Five cultures were included in each replication. In total, 680 (17 × 2 × 4 × 5) tubes were cultured. The experiment was repeated thrice. After eight weeks, the developed microtubers were harvested inside the clean bench and weighed in a mettler P-163 electric balance. The data on different parameters were collected and statistically analysed. The mean separation was done by LSD.

Source of Information: Plant Tissue Cult.& Biotech. 15(2): 157-166, 2005 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

From this study it is evident that microtuberisation of potato is mainly genotype source-dependent, though interaction of other factors such light, dark, temperature, chemicals, etc are there. The present results indicate that genotypes obtained from different sources may require separate protocol of microtuberisation for yield optimization. Moreover, the results obtained in the present study would help us in future planning for microtuber production with these valuable materials.

Knowledge Management: Journal