A total of 40 rice genotypes of Boro season selected from the germplasm bank of Bangladesh Rice Research Institute (BRRI), Gazipur viz. Cunail, Dholi boro, Ghuni boro, Kaikka boro, Deshi boro, Soloi, Sada boro, Kali boro 80/9, Jhatisail, Khaia boro, Boro jagli, Begun bitchi, Bana jira, Bash boro, Gochi, Rajasail, Jajli, Poshusail, Jagli, Bawoi, Bogra boro, Jagli boro, Tupa, Borya, Lafai, Sail boro, Biron, Kali boro 4/1, Kali boro 109/4, Kali boro 139/2, Kali boro 200, Kali boro 208, Kali boro 259, Kali boro 576, Kali boro 600, Boro, Boro, Boro 102/3, Kali boro 138/2 were grown under irrigated condition for genetic divergence study. The germplasm were grown with three replications using one row of 5.4 m long each per entry and single seedling per hill with a spacing of 25 X 20 cm between rows and plants, respectively. Fertilizers were applied @ 80:10:60 kg NPK/ha. Control measures for pests, diseases and weed were taken whenever necessary. Fourteen morpho-agronomic and yield contributing characters viz. flag leaf length (cm), flag leaf width (cm), plant height (cm), days to 50% flowering, days to maturity, panicle/hill(no.), panicle length (cm), filled grains/panicle, unfilled grains/panicle, grain length (mm), grain breadth (mm), length-breath ratio, 1000 grain weight (g) and yield/hill (g) were recorded to study the genetic diversity. Mahalonobis’ D2 and Canonical Vector Analysis methods were used to group the varieties based on their yield contributing and ancillaries characters using GENSTAT 5.13 program in computer.