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Efficacy of Seed Washing with Water and Treatment with Garlic Extract and Carbendazim for Reducing Seed-borne Fungal Pathogens from Rice Variety Kalijira

M. S. I. Uzzal
MS Student
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. S. Islam
MS Student
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

K. Mallick
MS Student
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. S. Monjil
Associate Professor
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

Seed health of aromatic rice variety Kalijira collected from Mymensingh Sadar Upazilla was studied following standard blotter method. Six different treatments namely water wash, garlic (1 : 1), water wash + garlic (1 : 1), Knowin 50WP (Carbendazim) @ 0.2% and water wash + Knowin-50WP @ 0.2% were used in this experiment. The identified seed-borne fungi were Alternaria padwickii, Aspergillus niger, Aspergillus flavus, Bipolaris oryzae, Curvularia lunata, Fusarium moniliforme, Fusarium oxysporum and Penicillium sp. Highest seed infection (30.67%) was recorded for F. oxysporum and the lowest seed infection (9.33%) was recorded for A. padwickii. Germination of seeds significantly varied in different treatments that ranged from 85.33 to 98.86%. Among the treatments, the highest (98.86%) germination was found by water wash + Knowin-50WP and lowest (85.33%) by control (untreated). After washing of seeds in water, the treated seeds with plant extract or chemical showing better performance than when only treated with plant extract or chemical for controlling seed borne pathogens.

Keywords: Rice, Seed-borne fungi, Washing with water, Garlic exactract

Location: Mymensingh Sadar Upazila of Mymensingh district

Start Date: 00-01-2012

End Date: 00-05-2013

Program Area: Pest Management

Commodity: Rice

Objectives:

To know the efficacy of seed washing with water and treatment with garlic extract and Carbendazim for reducing seedborne fungal pathogens.

Methodology:

Farmer's seeds of rice variety Kalijira were collected from Mymensingh Sadar Upazila of Mymensingh district. The seeds were then kept in brown paper bags and stored in the refrigerator at 4oC in Seed Pathology Center (BAU) until use. The experiment was conducted during the period from January 20l2 to May, 20l3 at Seed Pathology Center (SPC), Plant Disease Clinic and M. S. Laboratory, Department of Plant pathology, Bangladesh Agricultural University (BAU), Mymensingh. For the experiment seeds were treated with water, garlic extracts and fungicides. The treatments were T₁ = Water wash, T₂ = Garlic extract (1 : 1), T₃ = Water wash+ garlic extract (1:1), T₄ = Knowin 50 WP (Carbendazim) @ 0.2%, T5 = Water wash + Knowin 50 WP @ 0.2% and T6 = control (untreated). Garlic extract was prepared. For water wash, 5g of seeds were washed with running tap water properly and then dried in open air for 5 hrs. Seed treatment with garlic extracts was done by dipping seeds in previously prepared garlic extracts (1:1) for 20 minutes. Required amount of Knowin 50 WP was used for a seed treating at the rate of 0.2% of seed weight. The treated and untreated seeds were tested for seed-borne fungi. Four replications were conducted for each sample. After incubations, the germination of seeds and yielded fungi were counted following the keys outlined. For proper identification of fungi temporary slides were prepared from the fungal colony and observed under a compound microscope and identified with the help of keys. The treated Kalijira rice seeds were sown in trays containing sands for vigour test. After 10 days of sowing, shoot length and root length were measured and seedling vigor was calculated as vigor index. The collected all data were analyzed following computer package MSTAT-C and mean differences among the treatments were compared by Duncan's Multiple Range Test (DMRT).

Source of Information: Progress. Agric. 24(1 & 2): 45 – 51, 2013 ISSN 1017-8139

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

.Among the treatments, the highest (98.86%) germination was found by water wash + Knowin-50WP and lowest (85.33%) by control (untreated). After washing of seeds in water, the treated seeds with plant extract or chemical showing better performance than when only treated with plant extract or chemical for controlling seed borne pathogens.

Knowledge Management: Journal