Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Clinical Evaluation of Hyperimmune Serum for the Treatment of Newcastle Disease Inindigenous Layer Birds

M. S. Islam
Department of Medicine, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. S. Parvin
Department of Medicine, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

J. Akhter
Upazila Livestock Office
Islampur, Jamalpur, Bangladesh

M. T. Islam
Department of Medicine, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. P. Siddique
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202,Bangladesh

M. H. Rashid
Department of Microbiology, Jhenidah Government Veterinary College, Jhenidah, Bangladesh

Abstract/ Executive Summary:

The efficacy of hyperimmune serum for the treatment of Newcastle disease (ND) was evaluated in indigenous birds. A total of 20 indigenous birds (3 to 4 months old) were divided into three groups, namely group A (n = 9), B (n = 8) and C (n = 3). Birds of all the groups were infected orally with 0.2 mL (106.5EID50/0.1 mL) of virulent Newcastle disease virus (NDV). Birds of group A were treated with hyperimmune serum (raised in chickens against NDV) @ 2 mL/bird IM after 6 hours of infection before commencement of clinical signs. Birds of group B received two doses of hyperimmune serum, first @ 2 mL/bird IV after commencement of clinical signs and second @ 3 mL/bird IM 5 hours after first dosing. Birds of group C served as infected control. Birds of group A did not show any clinical signs of ND except one (11%). However, the only sick bird recovered after one repeat dosing of 1 mL hyperimmune serum. In group B, 87.5% birds survived with two birds having nervous signs. Therefore, it may be concluded that hyperimmune sera was effective in preventing morbidity and mortality due to ND in birds when administered before or after commencement of the clinical signs. However, it is needed to conduct a field trial to explore the efficacy of hyperimmune sera raised both in chickens and rabbits.

Keywords: Hyperimmune serum, Newcastle disease, Efficacy, Indigenous birds

Location: The experiment was conducted in the Department of Medicine in collaboration with the Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

Start Date: 00-07-2011

End Date: 00-06-2012

Program Area: Pest Management

Commodity: Chicken

Objectives:

To evaluate the efficacy of hyperimmune serum for the treatment of Newcastle disease in indigenous birds.

Methodology:

The experiment was conducted in the Department of Medicine in collaboration with the Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh during the period from July 2011 to June 2012. A total of 15 indigenous male birds of around 2-3 months of age and four New Zealand White rabbits (3-4 months old) were used for preparation of hyperimmune serum. All the birds and rabbits were purchased from local market and ICDDR, B, respectively. After acclimatization and deworming, all the birds were vaccinated thrice at 7 days interval with live attenuated NDV vaccine (Intervet) followed by a single administration of inactivated vaccine (Intervet) while rabbits were inoculated with inactivated vaccine (Intervet) only for 4 occasions. Blood samples were collected at different occasions of vaccination as well as after 10 days of last vaccination. Sera were separated and kept at -20°C until use.The haemagglutination inhibition (HI) test was done to determine the antibody titre in sera samples. The HI test was conducted according to the procedure described by earlier researchers. The antibody titre in pool sera was found to be very low (HI titre 192) in rabbits. So the rabbit serum was not used for treatment purpose. A total of 20 indigenous layer birds (3 to 4 months old) were divided into three groups, namely A (n = 9), B (n = 8) and C (n = 3). Each bird of all the groups was infected with 0.2 mL (106.5 EID50/0.1 mL) of virulent field isolate of Newcastle disease virus (NDV) through oral route of inoculation.Birds of group A were treated with hyperimmune serum (HI titre 512) @ 2 mL/bird IM after 6 hours of infection before commencement of clinical signs. However, one bird received repeat dose of 1 mL hyperimmune serum IM as it showed clinical signs 20 hours postinfection. Birds of group B were treated with hyperimmune serum @ 2 mL/bird IV after commencement of clinical signs. As no improvement was observed, these birds received repeat dose of 3 mL hyperimmune serum IM 5 hours after first dosing. Birds of group C served as infected control. Birds were monitored twice daily for 15 days to record clinical signs (if any) and mortality. Birds those died were subjected to post-mortem examination.

Source of Information: Progress. Agric. 24(1 & 2): 79 – 84, 2013 ISSN 1017-8139

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In conclusion, hyperimmune serum was found very effective in preventing morbidity and mortality of birds due to Newcastle disease, which gave a clear indication of effective use of hyperimmune sera in the protection of birds against ND, particularly the valuable birds like breeder and pet birds. However, it is needed to conduct a field trial to explore the efficacy of hyperimmune sera raised both in chickens and rabbits.

Knowledge Management: Journal