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Molecular Epidemilogic Study on Rota-viruses Causing Sever Diarrhea in Man, Animal and Poultry of Bangladesh

Dr. Md. Mahbub Alam
Associate Professor
Department of Medicine, BAU, Mymensingh-2202

M. N. Alam/ N. Kobayashi
Department of Medicine, BAU, Mymensingh-2202. Department of Hygiene, School of medicine, Sapporo Medical University, Sapporo, Japan.

Abstract/ Executive Summary:

0rotavirus is distributed worldwide as an enteric pathogen in man, animal and poultry. In this study the epidemiology of rotavirus infection in human, calves, sheep, goats and poultry were studied. A total of 800 fecal samples were collected from July 2010 to June 2011. These 800 samples were consisted to 330 human, 125 calves, 82 sheep, 7 goats and 267 poultry and examined by Polyacrylamide Gel Electrophoresis and Silver Staining (PAGE-SS) technique for the detection of presence of rotavirus dsRNA. Human rotavirus was detected in 10.94% (35/320) diarrheoeic fecal samples. The highest prevalence was recorded in September 33.33% and the lowest in May 4.54%. the prevalence of rotavirus infections was 33.33% in autumn (August-September), 11.69% in late autumn (October-November), 9.6% in winter (December-January), 9.72% in spring (February – March), 6.12% in summer (April-May) season and no detection was in rainy season (June – July) in diarrhoeic samples indicates the presence of rotavirus in infection in human. No calves, sheep and goat fecal sample was found positive for rotavirus of PAGE-SS technique. During the study period, 267 fecal samples were tested of chicks and only one was found positive for rotavirus infection (0.38%; 1/267) was found positive for rotavirus on PAGE-SS technique. The positive sample was detected from 10 days layer chicks in the winter season. The migration patterns of detected positive strains were not similar in polyacrylamide gel electrophoresis and their migration speed was different types. Five electropherotypes were determined among 35 human rotavirus. All the electropherotypes were long pattern. The genome migration of avian rotavirus type was distinct from human types. In the present study, it was not investigated that bacteria, parasites or any other pathogens responsible for causing diarrhea or not. Further work is in progress.

Keywords:

Location: BAU-Mymensingh, Medical College Hospital-Mymensingh, Over all Bangladesh

Start Date: 01-07-2010

End Date: 30-06-2012

Program Area: Animal Health and Management

Commodity:

Objectives:

1. To study the prevalence of Rotaviruses from diarrhea faecal samples of man, animals and poultry in Bangladesh 2. To determine the distribution of G and P serotypes of rotaviruses circulating in Bangladesh 3. To characterize and clarify genetic diversity of Rotaviruses in man, animals, and poultry in Bangladesh.

Methodology:

Source of Information:

Article Link (Online Journal):

Funding Source:

1.	Budget: 240000

Total Budget (Tk.) : 240000

Achievement/Findings:

Knowledge Management: Report/Proceedings