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Effect of Probiotic on Beneficial and Pathogenic Microorganisms in the Gastrointestinal Tract of Commercial Broiler with Bcrdv Vaccine

M. R. Islam
Department of Pharmacology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. Mostofa
Department of Pharmacology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

K. S. Mondal
Department of Pharmacology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

R. R. Roy
Department of Pharmacology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. G. Sorwar
Department of Pharmacology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

S. Islam
Department of Pharmacology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The present study was conducted to determine the effect of probiotic on beneficial and pathogenic microorganisms in the gastrointestinal tract of Commercial Broiler. A total of 80 day-old broiler chicks were divided into two main groups I and II with 40 chicks in each. Group I was supplemented with Probiotic (Saccharomyces cerevisiae 2 gm/kg feed) containing feed and chicks of group II was supplemented with conventional feed. Group I and II was again subdivided into two subgroups as group A, B and C, D respectively on 6 days of age. Birds of subgroups A and C were vaccinated with ND vaccine (BCRDV) and birds of subgroups C and D were kept as unvaccinated. The result evidenced the following information: (a) The total viable count (TVC) and total coliform count (TCC) were significantly (P<0.01) lower in experimental birds as compared to control at 14, 28 and 32 days of age both in vaccinated and non-vaccinated birds. It was always found that the lower TVC in probiotic fed broilers as compared to conventional fed broilers (b) The total lactobacilli count (TLC) was significantly (P<0.01) higher in experimental birds as compared to control at 14, 28 and 32 days of age both in vaccinated and nonvaccinated birds. So it can be said that Saccharomyces cerevisiae supplementation can reduce the pathogenic microorganism and increase the growth of beneficial microorganism.

Keywords: Probiotic, Broiler, Bacterial count

Location: Bangladesh Agricultural University (BAU), Mymensingh.

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Chicken

Objectives:

To evaluate the effect of probiotics with regard to clearing bacterial infections and regulating intestinal flora by determining the total viable count (TVC) and total lactobacillus count (TLC) of the crop and cecum samples of probiotics and conventional fed groups

Methodology:

Day-old “Ross 308” broiler chicks were purchased from a market of Gazipur, Bangladesh and transported carefully to the Poultry Shed of the Department of Pharmacology, Bangladesh Agricultural University (BAU), Mymensingh, in well-ventilated paper cartons. The birds were reared under hygienic management practices throughout the entire period of study. Commercially available poultry feed (Quality Feeds Ltd., Dhaka) was used throughout the experimental study. The broiler chicks were fed with standard broiler starter up to 14 days and broiler grower from 15-32 days of age, as formulated by Quality Feeds Ltd., Dhaka. The live body weight of five birds from each subgroup was taken with the help of standard balance. The body weight of birds was taken at 7 days interval from day 0 to 32. Crop and cecum were used as samples for bacterial culture. Portions of crop and cecum with their contents were obtained aseptically with a sterile scalpel and forceps.For the determination of total bacterial counts, 0.1 ml of each ten-fold dilution was transferred and spread on triplicate PCA or NA agar using a sterile pipette for each dilution. The diluted samples were spreaded as quickly as possible on the surface of the plate with a sterile glass spreader.For the determination of total lactobacillus count and total coliform count the procedures of sampling, dilution and Streaking was similar to those followed in the total bacterial count. Only in case of lactobacillus count MRS agar and of total coliform count MacConkey agar was used.The examination followed detail study of cultural characteristic including colony formation, staining reactions and biochemical properties. In order to find out different types of microorganisms in meat different kinds of bacterial colonies were isolated in pure culture and identified. Carbohydrates fermentation test, IMViC test for enteric bacteria, TSI agar slant reaction, Motility test, Maintenance of stock culture were determined. Data were analyzed using SPSS v.10 for Windows (SPSS Inc., Chicago, IL, USA). Statistically significant differences between group means were determined by analysis of variance (ANOVA). When the differences were significant, Duncan’s multiple range tests was performed. Mean values were considered significantly different at P<0.05. Data are expressed as mean values ± SEM.

Source of Information: Progress. Agric. 24(1 & 2): 107 – 115, 2013 ISSN 1017-8139

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Probiotic increases the beneficial microorganisms and decreases the pathogenic microorganisms in the gastrointestinal tract of commercial broiler.

Knowledge Management: Journal