Agricultural Research Management Information System

  • Home
  • Research Summary
    • All
    • Government Organization
      • Agriculture Training Institute, Ishwardi, Pabna
      • Bangabandhu academy for poverty alleviation and rural development (BAPARD)
      • Bangabandhu Sheikh Mujibur Rahman Science & Technology University
      • Bangladesh Bureau of Statistics
      • Bangladesh Institute of Health Sciences
      • Bangladesh Institute of Tropical & Infections Diseases (BITID)
      • Bangladesh Meteorological Department
      • Bangladesh National Herbarium
      • Bangladesh Space Research and Remote Sensing Organization
      • Bangladesh Technical Educational Board
      • Barind Multipurpose Development Authority
      • Central Cattle Breeding Station
      • Department of Agriculture Extension
      • Department of Fisheries
      • Department of Livestock Services
      • Department of Youth Development
      • Dhaka Medical College
      • Geological Survey of Bangladesh
      • Institute of Epidemiology, Disease Control & Research
      • Jatiya Kabi Kazi Nazrul Islam University
      • Khulna Govt. Women College
      • Livestock Training Institute
      • Local Government Engineering Department
      • Ministry of Agriculture
      • Ministry of Environment and forest
      • Ministry of Fisheries and Livestock
      • Ministry of Labour & Employement
      • Ministry of Land
      • Ministry of Public Administration
      • Ministry of Textiles and Jute
      • Ministry of Water Resources
      • Ministry of Youth and Sports
      • National Agricultural Training Academy
      • National institute of preventive and social medicine
      • National Mushroom Development and Extension Centre
      • Pabna University of Science and Technology
      • Seed Certification Agency
      • Shaheed Suhrawardy Medical College
      • Sheikh Hasina University
      • University Grants Commission
      • Youth Training Centre
    • Autonomous/Semi-gov Org
      • Bangladesh Academy for Rural Development
      • Bangladesh Agricultural Development Corporation
      • Bangladesh Atomic Energy Commission
      • Bangladesh Council of Scientific and Industrial Research
      • Bangladesh Fisheries Development Corporation
      • Bangladesh Institute of Development Studies
      • Bangladesh Institute of Management
      • Bangladesh Milk Producers Cooperative Union Limited
      • Bangladesh Water Development Board
      • BIRDEM
      • Center for Environmental and Geographic Information Services
      • Hortex Foundation
      • Institute of Water Modeling
      • National Institute of Biotechnology
      • River Research Institute
      • Rural Development Academy
    • NARS
      • Bangladesh Agricultural Research Council
      • Bangladesh Agricultural Research Institute
      • Bangladesh Fisheries Research Institute
      • Bangladesh Forest Research Institute
      • Bangladesh Institute of Nuclear Agriculture
      • Bangladesh Jute Research Institute
      • Bangladesh Livestock Research Institute
      • Bangladesh Rice Research Institute
      • Bangladesh Sericulture Research and Training Institute
      • Bangladesh Sugarcrop Research Institute
      • Bangladesh Tea Research Institute
      • Bangladesh Wheat and Maize Research Institute
      • Cotton Development Board
      • Soil Resource Development Institute
    • Public University
      • Ahsanullah University of Science and Technology
      • Bangabandhu Sheikh Mujibur Rahman Agricultural University
      • Bangamata Sheikh Fojilatunnesa Mujib Science and Technology University
      • Bangladesh Agricultural University
      • Bangladesh Open University
      • Bangladesh University of Engineering and Technology
      • Bangladesh University of Professionals
      • Bangladesh University of Textiles
      • Barisal Government Veterinary College
      • Begum Rokeya University
      • Chittagong University of Engineering and Technology
      • Chittagong Veterinary and Animal Science University
      • Comilla University
      • Dhaka University of Engineering & Technology
      • Dinajpur Government Veterinary College, Dinajpur
      • Gono Bishwabidyalay
      • Hajee Mohammad Danesh Science and Technology University
      • Islamic University, Kushtia
      • Jagannath University
      • Jahangirnagar University
      • Jessore University of Science and Technology
      • Jhenaidha Government Veterinary College
      • Khulna Agricultural University
      • Khulna University
      • Khulna University of Engineering & Technology
      • Mawlana Bhashani Science and Technology University
      • Millitary Institute of Science and Technology
      • National University
      • Noakhali Science and Technology University
      • Patuakhali Science and Technology University
      • Rajshahi University of Engineering and Technology
      • Shahjalal University of Science & Technology
      • Sher-e-Bangla Agricultural University
      • Sylhet Agricultural University
      • Sylhet Government Veterinary College
      • University of Barisal
      • University of Chittagong
      • University of Dhaka
      • University of Rajshahi
    • Private University
      • Asian University of Bangladesh
      • Atish Dipankar University of Science and Technology
      • BGC Trust University Bangladesh
      • BGMEA University of Fashion & Technology (BUFT)
      • BRAC University
      • City University
      • Daffodil International University
      • East West University
      • Exim Bank Agricultural University
      • Gana Bishwabiddalaya
      • Hamdard University
      • Independent University, Bangladesh
      • International Islamic University Chittagong
      • International University of Business Agriculture and Technology
      • Islamic University of Technology
      • Leading University, Sylhet
      • North South University
      • Premier University
      • Primeasia University
      • Private University
      • SOAS, University of London
      • Southeast University
      • Stamford University
      • State University of Bangladesh
      • The Millenium University
      • University of Asia Pacific
      • University of Development Alternative
      • University of Information Technology and Sciences
      • University of Liberal Arts Bangladesh
      • University of Science and Technology, Chittagong
      • World University
    • INGO/IO/NGO/Private Org
      • ACI Limited
      • Agricultural Advisory Society (AAS)
      • Apex Organic Industries Limited
      • Arannayk Foundation
      • Bangladesh Academy of Sciences
      • Bangladesh Centre for Advanced Studies
      • Bangladesh Institute of Social Research
      • Bangladesh Science Foundation
      • Bangladesh Unnayan Parishad
      • BAPA
      • BRAC
      • CARE Bangladesh
      • CARITAS
      • Centre for Environmental Geographical Information System
      • Centre for Policy Dialogue (CPD)
      • Creative Conservation Alliance
      • Dhaka Ahsania Mission
      • Dwip Unnayan Sangstha
      • EMBASSY OF DENMARK, BANGLADESH
      • Energypac Limited Bangladesh
      • FAO- Bangladesh
      • FIVDB
      • ICDDRB, Mohakhali, Dhaka-1212
      • iDE Bangladesh
      • Innovision Consulting Private Ltd.
      • International Center for Climate Change and Development
      • International Centre for Integrated Mountain Development
      • International Development Research Centre
      • International Fertilizer Development Center, Bangladesh
      • International Food Policy Research Institute
      • International Maize and Wheat Improvement Centre
      • International Potato Center
      • IRRI- Bangladesh
      • IRRI-Philippines
      • Ispahani Agro LTD
      • IUCN, Bangladesh
      • Krishi Gobeshina Foundation
      • Lal Teer
      • Mennonite Central Committee
      • Metal (Pvt.) Ltd
      • Modern Herbal Group
      • Palli Karma-Sahayak Foundation
      • Practical Action Bangladesh
      • Proshika
      • RDRS Bangladesh
      • RIRI-Philippines
      • Rothamsted Research
      • SAARC Agricultural Centre
      • SAARC Meteorological Research Centre
      • Social Upliftment Society
      • South Asia Enterprise Development Facility
      • Square Pharmaceuticals Ltd.
      • Supreme Seed
      • Transparency International Bangladesh
      • Unnayan Onneshan
      • USAID
      • Water Resources Planning Organization
      • Winrock International
      • World Bank
      • World Food Program
      • World Vegetable Center
      • WorldFish Centre, Bangladesh
    • Foreign University
      • Asian Institute of Technology
      • Auckland University of Technology
      • Australian National University
      • Bidhan Chandra Krishi Viswavidyalaya
      • BOKU-University of Natural Resources and Applied Life Sciences
      • Cranfield University
      • Curtin University
      • Foreign University/ Institute
      • Hiroshima University
      • Hokkaido University
      • Huazhong Agricultural University
      • International Islamic University, Malaysia
      • Kagawa University
      • Kangwon National University
      • Kochi University
      • Kyoto University
      • Kyushu University
      • Ladoke Akintola University of Technology
      • Murdoch University
      • Nagoya University
      • NOAA-CREST, CCNY
      • Royal Veterinary and Agricultural University
      • San Diego State University
      • Shinshu University
      • Tottori University
      • United Nations University
      • University Malaysia Kelantan
      • University Malaysia Pahang
      • University Nova de Lisboa
      • University of Alberta
      • University of Bremen
      • University of Bremen
      • University of Calgary
      • University of california
      • University of Greenwich
      • University of Hamburg, Hamburg
      • University of Hannover
      • University of Hawaii
      • University of Helsinki, Finland
      • University of Kalyani
      • University of Leeds
      • University of Liverpool
      • University of Malaya
      • University of Milan
      • University of New England
      • University of Philippines
      • University of Plymouth
      • University of Queensland
      • University of Reading
      • University of Southampton
      • University of Texas
      • University of the Punjab
      • University of Tokyo
      • University of Toronto
      • University of Wales
      • University of Washington
      • University of Wollongong
      • University Putra Malaysia
      • University Sains Malaysia
  • Search
    • Search by Keyword
    • Search by Organization
    • Search by Program Area
    • Search by Commodity/Non-commodity
    • Search by Funding Source
    • Search by Researcher
    • Custom Search
    • On-going Research
  • About Us
    • ARMIS
    • Brochure
  • Contact Us
    • BARC Personnel
    • ARMIS Personnel
    • Feedback
  • Report
    • All
    • By Organization
      • Bangladesh Agricultural Research Council
      • Bangladesh Agricultural Research Institute
      • Bangladesh Fisheries Research Institute
      • Bangladesh Forest Research Institute
      • Bangladesh Institute of Nuclear Agriculture
      • Bangladesh Jute Research Institute
      • Bangladesh Livestock Research Institute
      • Bangladesh Rice Research Institute
      • Bangladesh Sericulture Research and Training Institute
      • Bangladesh Sugarcrop Research Institute
      • Bangladesh Tea Research Institute
      • Bangladesh Wheat and Maize Research Institute
      • Cotton Development Board
      • Soil Resource Development Institute
    • Research Trend Analysis
  • User Request
  • Data Input
  • Help
    • Operation Manual
      • PDF
      • Video
    • Program Area & Commodity
  • We have reached 37600 number of research entries at this moment.
    • Logout

Research Detail

  1. Home
  2. Research
  3. Detail
S. M. H. Jahan
Department of Entomology, Patuakhali Science and Technology University, Dumki, Patuakhali - 8602. Bangladesh

K-Y. Lee
Depart ment of Agricultural Biology, Kyungpook National University, Daegu 702-70 I. Korea

M. A. Rahman
Department of Entomology, Patuakhali Science and Technology University, Dumki, Patuakhali - 8602. Bangladesh

M.I. A. Howlader
Senior Scientific Officer, On-Farm Research Division, Bangladesh Agricultural Research Institute, Patuakhali-8600. Bangladesh.

M. F. Hasan
Department of Horticulture, Patuakhali Science and Technology University, Dumki. Patuakhali-8602. Bangladesh

Whiteflies are associated with several secondary endosym biotic bacteria, which are vertically transmitted from mother to progeny , that have major effects on host preferences, physiology, and evolution. Here, we identified Arsenophonus bacteria in Bemisia tabaci as well as other whitefly popluations from different countries by comparison of 23S rDNA sequences. Arsenophonus were detected in all tested indigenous B. tabaci populations as well as Q I biotype of Korea, whereas they were absent from B biotype of Korea and Q biotype of China. Arsenophonus were also detected in all seven tested Trialeurodes aporariorum populations as well as Terraleurodes acaciae , whereas they were not detected in a ll five Aleurodicus dispersus populations. Among the 19 whitefly populations from different countries, our analysis identified five phylogenetic groups of Arsenophonus, thereby demonstrating the high diversity of this genus. Arsenophonus phylogeny suggests a correlation of geographical range with ecological variation at the species level.

  Arsenophonus, Population diversity , Secondary endosym biont, Sweetpotato, Whitefly
  Host plants,such as tomato, pepper,ridge gourd,bean,okra, eggplant, and guava, grown in Bangladesh,Myanmar, Nepal, China, the Philippines, and Korea
  00-11-2011
  00-04-2011
  Pest Management
  Insects

1. To determine the geographic distribution of Arsenophonus infection in B. tabaci and other whiteflies as well as the relationship between Arsenophonus infection and evolutionary linkage in whiteflies.

Adult individuals of B. tabaci and other whiteflies were collected from various host plants, such as tomato, pepper, ridge gourd, bean , okra, eggplant, and guava, grown in Bangladesh, Myan mar, Nepal, China, the Philippines, and Korea. In Bangladesh , we collected bean, okra, and eggplant from the southern and northern parts of the country during 2010-2011 , immediately preserved samples in 99% ethanol , and stored them at -20°C for further analysis. Adults of B. tabaci B and Q biotypes were collected from cucumber, sweet melon , and tomato plants grown throughout Korea in 2010 in order to compare the morphologies and genetic sequences of foreign whiteflies.Biotype of B. tabaci was determined by amplification of mitochondrial COi gene fragments from extracted genomic DNA samples. The presence of Arsenophonus in whiteflies was determined using specific primer sets for Arsenophonus by amplification of 23S rDNA gene fragments. The occurrence of TYLCV on cultivars of various horticultural crops was surveyed in different regions . TYLCV acquisition by B. tabaci was determined using a TYLCV-specific primer set that can amplify conserved intergenic sequences. PCR reactions were performed in a 20 mlmixture containing 5x SuperTaq PCR buffer (10 mM Tris-HCL, 40 mM KCI , l.5 mM MgC12 , pH 9.0), 2.5 mM dNTPs, 0.5 M of each primer , 1 u nit of SuperTaq DNA pol ymerase (SuperBio Co, Korea), and 1 mg of DNA as a tem plate. The mixtures were amplified using a PTC-200 thermal cycler (MJ Research , Watertown, MA, USA) wi th 5 min of initial denaturation at 95°C, 35 cycles of annealing (30 sec at 95°C, 30 sec at 60°C, 45 sec at 72°C), and 10 min of extension at 72°C. The PCR products were visualized on a 1 .0% agarose gel containing ethidium bromide. Expected PCR products were excised from the gel and purified using the Wizard PCR preps DNA purification system (Promega, Madison, WI, USA) and sequenced either directly or by cloning into pGEM-T easy plasmid vector.Sequences of the PCR products were determined using a Big Dye Terminator Cycle Sequencing Kit (Appl ied Biosystems, Foster City, USA) and analyzed using a 3730XL DNA Sequencer (Applied Biosystems , Foster City , USA). Databases were searched using the BLAST algorithm in NCBI, and sequences were aligned using the MUSCLE program. The 23S ribosomal DNA sequences of Arsenophonus were analyzed using Bayesian Mr Bayes 3.0 software. Four Metropolises-cou pled Markov Chain Monte Carlo (MCMC) chains were run until standard divergence of the split frequencies become lower than 0.0 l. A ll sequences were analyzed over 10 million generations, and four sequences were sampled every 100 generations. The first 25 % of burn-in (SUMP and SUMT) cycles were discarded prior to the construction of consensus tree, which were visualized by MEGA 4 0.

  J Patukahli Sci and Tech. Univ. 2013, 4(2) : 129-141; ISSN 1996-4501
  
Funding Source:
  

The present study shows that all of the B. tabaci populations collected from host plants in Bangladesh, Myanmar, and Nepal were infected by Arsenophonus, whereas the Q biotype population from China and B bioty popopulation from Korea were not according to the 23S rDNA sequence analysis, we identified five phylogenetic clades, illustrating the divergence of Arsenophonus endosymbiont.

  Journal
  


Copyright © 2025. Bangladesh Agricultural Research Council.