Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Studies on the Isolation of Parahydroxy Benzoic Acid from the Leaves of Cassia Alata

M. S. Rahman
BCSIR Laboratories, Binodpur Bazar, Rajshahi- 6206, Bangladesh

A.J.M Moynul Hasan
BCSIR Laboratories, Binodpur Bazar, Rajshahi- 6206, Bangladesh

M. Y. Ali
BCSIR Laboratories, Binodpur Bazar, Rajshahi- 6206, Bangladesh

M. U. Ali
BCSIR Laboratories, Binodpur Bazar, Rajshahi- 6206, Bangladesh

Abstract/ Executive Summary:

Studies were carried out on the leaves of Cassia alata. Parahydroxy benzoic acid was isolated from the leaves of Cassia alata with the help of column and thin layer chromatography using a gradient of organic solvents with increasing polarity. The compound was characterized on the basis of UV, IR, 1H-NMR and Mass spectrometry.

Keywords: Isolation, Parahydroxy benzoic acid, Leaves of cassia alata

Location: BCSIR Laboratories, Binodpur Bazar, Rajshahi- 6206, Bangladesh

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

The present work had, therefore, been taken with a view to carry on a complete investigation for the isolation of compounds other than anthraquinones from the leaves of Cassia alata and accordingly Parahydroxy benzoic acid was isolated from the plant.

Methodology:

A Reichart micro melting point apparatus was used for recording the melting point. UV spectra (MeOH) was recorded on a Shimadzu UV-240 spectrophotometer, IR spectra (KBr) on a Shimadzu IR-460 instrument, 1H-NMR spectra (CD3OD) on a Bruker AM-500 FT NMR spectrometer (500 MHz) using TMS as internal standard and Mass spectra on a Varian-MAT 112S spectrometer. Electron Impact (EI) and Peak Matching experiments were performed on a MAT-312A mass spectrometer. Fresh leaves were collected from the plants grown in the adjoining areas of BCSIR Laboratories, Rajshahi campus during August-September period. The leaves were washed with water to remove extraneous materials and then dried in shade. Care was taken to avoid exposure to sunlight. The dried material was crushed to powder. The air-dried Cassia alata leaf powder (6.60 Kg) was soaked in 80 % ethanol for a week. The ethanolic extract was then filtered and the solvent was removed under reduced pressure to obtain a viscous residue (487 g). The crude ethanolic extract was then defatted with n-hexane. The n-hexane solvent was then removed under reduced pressure to yield the residue (160 g). The defatted extract was then treated with water, shaken well to resolve into water soluble and water insoluble parts. The water soluble part was extracted with ethyl acetate. The ethyl acetate soluble part was chromatographed over a silica gel (70-230 mash) column and successively eluted using increasing polarity of ethyl acetate and methanol. Elution of the column with ethyl acetate : methanol (60 : 40 v/v) afforded compound 1 along with minor impurities. Purification of the compound by Preparative thin layer chromatography (PTLC) The compound 1 with some impurities was applied to a PTLC card of silica gel 60GF254 (thickness 0.1mm) and eluted with ethyl acetate : methanol (9 :1 v/v). Adistinct single band (Rf 0.69) was observed on the PTLC card. The band was collected and washed out with ethyl acetate to obtain a crystalline solid (compound 1, 10.9 mg, m.p 215 - 216O C, Rf = 0.69). Spectroscopic analysis of compound 1 Uvλmax (MeOH)nm : 254, 203, 196 IRυmax (KBr) cm-1 :3350 (O - H), 1445, 840 (C-H), 1675 (C = 0), 1601 (C = C) EIMS m/z (rel. int %) : 138(74), 121(100), 110(1), 93(26), 81(3), 65(32), 53(7). Peak matching m/z (formula) : 138.02643 (C7H6O3).

Source of Information: Bangladesh J. Sci. Ind. Res. 41(1-2), 89-92, 2006

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

From the exact matching of the UV, IR, 1HNMR and mass spectral evidences compound 1 was characterized as Parahydroxy benzoic acid.

Knowledge Management: Journal