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Hematological Parameter of Heteropneustes Fossilis (shing Fish)

Tahmina Khanam
Assistant Professor
Department of Biotechnology and genetic engineering,University of Development Alternative, Dhaka

Dr. Gulshan Ara Latifa
Professor
Department of Zoology, Dhaka University, Dhaka

Abstract/ Executive Summary:

The physiological conditions of fish are essential for the successful fish culture. Objective: The purpose of the present study was to see the haematological parameter of air breathing cat fish (Heteropneustes fossilis) of Bangladesh. Methodology: This animal study was conducted in the “Bargen lab” in the Department of Zoology at University of Dhaka as well as in “The Peoples Pathological lab” which was a private diagnostic laboratory at Dhaka city from July’ 2008 to April’ 2009 for a period of 9(nine) months. The fish was Heteropneustes fossilis. Haemocytometer including two graduated pipettes was used for counting leucocytes. Result: The result indicated eight types of blood cells in peripheral condition of Heteropneustes fossilis. The average cellular counts of Heteropneustes fossilis were erythrocytes 8.45X10⁶ m³, leukocytes 15.44X10³m^-3, Thrombocytes 34.72%, large lymphocytes 1.02%, small lymphocytes 26.7%, monocyte 3.9%, neutrophil 16.9%, eosinophils 6.97%, basophiles 8.6%, haemoglobin 11.7g 100ml¹, erythrocyte sedimentation rate (ESR) 6.49g 100ml^-1. The most of the hematological parameter showed intra specific variation except eosinophils and ESR of Heteropneustes fossilis (Shing fish). Conclusion: Hematological studies shows that the physiology of fish change with the change in the environment, time, season, maturity, nutritional state, activity level physical and chemical change in water.

Keywords: Hematology, E.S.R., Heteropneustes fossilis, EDTA, DC

Location: Department of Zoology at University of Dhaka

Start Date: 00-07-2008

End Date: 00-04-2009

Program Area: Animal Health and Management

Commodity: Cat fish

Objectives:

To see the change in hematological parameters of air breathing cat fish (Heteropneustes fossilis) due to seasonal variation

Methodology:

This animal experimental study was conducted in the “Bargen lab” in the Department of Zoology at University of Dhaka as well as in “The Peoples Pathological lab” which was a private diagnostic laboratory at Dhaka city from July’ 2008 to April’ 2009 for a period of 9(nine) months. The fish was Heteropneustes fossilis which belongs to Heteropneustidae family was examined. Ethylene Diamine Tetra Acetic acid (EDTA) was used as anticoagulant. Haemocytometer, injection needle, electronic microscope, slides, reagent bottles, beakers, micropipettes, petri dish, glass road stinger and cloth were used. Haemocytometer including two graduated pipettes was used for counting leucocytes. At first blood was drawn exactly up to the marks 0.5 in a clean dry WBC pipette; then blood was wiped off from the outside of the pipette. After that, white cell was sucked and was diluted third exactly up to 11 mark. Air bubble was prevented to enter into the bulb; then the blood was mixed thoroughly followed by vigorous rotation was done to dilute the fluid in the bulb. Then the special cover slip was placed on the platform of the counting chamber followed by firm pressure till a service of concentric rings appears. Then the diluting fluid of the long stem was discarded which was about 1/3 of the bulb. The tip of the pipette was touched on the platform at the edge of special cover slip at about 45 angles. The chamber was filled by surface tension. Then it was kept in the counting chamber for 2 to 3 minutes for the cells to settle. The chamber was placed on the microscope and was focused the rulings under the microscope. The total numbers of leucocytes were estimated.

Source of Information: J. Sci Found, 2013;11(1):3-10

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The physiological changes are better understood by the changes in the blood composition. For diagnosis of physiological disorders and effects of environmental stresses and diseases of the various kinds, study and understanding of the blood physiology of the fish requires an instristic knowledge of the composition and function of the varies blood component in nature and under specific environmental condition to enable the establishment of a basic haematological profile for future use. However to establish the blood value a thorough understanding of the normal range ,mean with standard deviation and their various with length group, weight group, physiological and physical condition, of cultured procedures of fresh water is needed. It may be concluded that this types observation is needed for future use in order to development of fisheries sector. So Haematological profile of Heteropneustes fossilis is important in evaluating the health of fish.

Knowledge Management: Journal