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Study of Shoot Multiplication of Strawberry (fragaria Ananassa)

Nayem Zobayer
Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology, Sylhet, Bangladesh

Shamsul H. Prodhan
Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology, Sylhet, Bangladesh

Saif U. Sikdar
Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology, Sylhet, Bangladesh

Fazle Azim
Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology, Sylhet, Bangladesh

M. Ashrafuzzaman
Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology, Sylhet, Bangladesh

Abstract/ Executive Summary:

Effects of different hormonal concentration on multiple shoot formation of RABI Strawberry-3 were studied. Where the explants were runner tips that cultured on MS basal medium supplemented with 6-Benzyl Adenine and Kinetin. The maximum percentage 80% of shoot was obtained from Benzyl Adenine concentrations of 0.5 mg/l and 1.0 mg/l whereas Kinetin concentrations were 1.5 mg/l and 2.0 mg/l separately. More promising result was found by combining two impressive concentrations of each hormone. When MS basal media was supplemented with Kinetin 2.0mg/l + Benzyl Adenin A 0.5 mg/l, Kinetin 1.5 mg/l + Benzyl Adenine 0.5 mg/l and Kinetin 1.5 mg/l + Benzyl Adenine 1.0 mg/l respectively then 100% shoots were obtained.

Keywords: RABI Strawberry-3, Benzyl Adenine, Kinetin, Shoot Formation and Regeneration

Location: Plant Genetic Engineering Laboratory of Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Strawberry

Objectives:

To study multiple shoot regeneration system from runner tips of RABI strawberry-3

Methodology:

Strawberry plantlets of RABI Strawberry-3 were collected from Rajshahi University. Healthy and vigorous runner tips were collected from the strawberry plants, which were used as explants. During transferring bud to solid sterile medium a whitish exudates of bacteria was observed around the base of the explants after 2-3 days and it was complicated further by the latent nature of the contaminants. The exudates were removed by placing the runner tips were placed in autoclaved beaker and washed with sterile distilled water. The runner tips were then kept in 70% of ethanol for 3-5 miniutes, followed by washing with autoclaved distilled water for several times. Runner tips were further sterilized by continuous shaking with adding Sodium hypochlorite with 2- 3 drops of tween-20 for 12-15 minutes. Surface sterilized runner tips were thoroughly washed five times with autoclaved sterile distilled water. The runner tips were then placed on the sterilized petridishes having sterilized filter paper to remove excess water from the runner tips. Runner tips were cultured on MS supplemented with specific concentration of growth regulators (BA and Kinetin). After surface sterilization apical meristems of 3-5 mm long were isolated and used as explants. Firstly, the growth regulators were supplemented in the media singly. Then, on the basis of performance two best concentrations were selected from each growth regulators. Surface sterilized runner tips were placed on solid MS media in a laminar airflow cabinet. In each test tube one runner tip was placed. The pH of the media was adjusted to 5.8 ± 2 before autoclaving. After inoculation, the surface sterilized runner tips of RABI Strawberry-3 variety was transferred and maintained in an environmentally controlled growth room for 3 weeks for multiple shoot generation and growth. The cultures were positioned away from continuous light provided by general electric white florescent tubes. Temperature was maintained at 25±2?C through the growth period. Multiple shoot generation and growth frequency was recorded 12-15 days after inoculation. Subculture was carried out once in every two weeks with transfer of only the vigorously growing explants. Experiments were replicated three times and forty test tubes with forty runner tips were used per replication for each type.

Source of Information: ISSN: 2224-0616 Int. J. Agril. Res. Innov. & Tech. 1 (1-2): 69-72, December, 2011 Available at http://www.ijarit.webs.com

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

For multiple shoots formation, lowest concentrations of BA i.e., 0.5 and 1.0 mg/l were outstanding. Combination of high concentrations of KIN i.e., 2.0 and 1.5 mg/l with lower concentrations of BA resulted more effectively than the single cytokinin treatment. As strawberry is still a new crop in Bangladesh so more research should be carried on to further improvement of this crop.

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