Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Studies on the Preparation of Powerful Phenyl from Petuli Seed Oil

M. I. H. Mondal
BCSIR, Laboratories, Binodpur Bazar, Rajshahi-6206, Bangladesh

M. A. Khaleque
BCSIR, Laboratories, Binodpur Bazar, Rajshahi-6206, Bangladesh

M. A. Hossain
BCSIR, Laboratories, Binodpur Bazar, Rajshahi-6206, Bangladesh

M. A. Hye
BCSIR, Laboratories, Binodpur Bazar, Rajshahi-6206, Bangladesh

Abstract/ Executive Summary:

Studies were carried out on the preparation of powerful phenyl from petuli (Trewia nudiflora linn) seed oil. It was found that petuli seed contains 22% of pale-yellow coloured oil. The physical and chemical characteristics of the oil were determined by the conventional methods and the fatty acid by gas liquid chromatography (G.L.C). From the results it is evident that the oil can be used suitably for the preparation of powerful phenyl on accounts of its high α-elaeostearic acid (38%) a major fatty acid component content and higher saponification value (185). This phenyl was applied on bacteria and found effective result.

Keywords: Phenyl, Petuli seed oil, Locally available raw material.

Location: BCSIR, Laboratories, Binodpur Bazar, Rajshahi-6206, Bangladesh

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

The object of the study was carried out with a view to prepare a powerful phenyl from neglected locally available raw material petuli seed oil.

Methodology:

Ripe matured petuli seeds were collected from locally. The seeds were then deshelled manually and the kernels thus obtained were crushed into smaller particles in a iron mortar and dried in the oven at a temperature of 105^OC for an hour. The oil was then extracted in a soxhlet apparatus with petroleum ether (40-60^O C) for 8-hours. The extracted solvent was, removed by a rotary vacuum evaporator under reduced pressure and calculated the percentage of the oil. The specific gravity of the oil was determined at 28^O C with the help of a pycnometer. Refractive index of the clear oil free from water and air bubbles was determined at 28^OC by the standard IUPAC method.7 Moisture and volatile matters in the oil were also determined by IUPAC method.7 The percentage of free fatty acid as oleic saponification value, peroxide value and unsaponifiable matters in the oil were determined by the standard AOCS method.8 Hanus method was followed to determine the iodine value of the oil.9 F.D.A (Food and Drug Administrative) method was used to determine the Antibacterial activity and MIC value.10 Analysis of fatty acids: The fatty acid composition of petuli seed oil was analyzed as their methyl esters, which was prepared by the Boron-trifloride methanol method.11 A GCD pye Unicom gas chromatograph equipped with a flame ionization detector was used to determine the fatty acid methyl esters. Nitrogen carrier gas was used at a flow rate of 30 ml/mn. Fatty acids were separated on a 1.8 m × 1/8 i.e. glass column packed with 6 % BDS (Batanediol Succinate polyesters) on solid support anakorm ABS (100/120) mesh was used. The column was operated isothermally at 190^OC. The injector and detector temperature were maintained at 230^OC for all GLC analysis.Gas chromatographic peaks were identified by comparison with standard esters with respect to retention times against equivalent carbon length (ECL). The peak areas were determined by a Pye Unicom electronic integrator. The percentage of each peak gas calculated as the percentage of total area of all the peaks. Preparation of powerful phenyl: 500 g rosin was melted in a stainless steal beaker of two litres capacity at 80^OC 250 g raw petuli seed oil was poured into it and heated to about 200^OC. Then added 400 ml of 25 % sodium hydroxide solution with it and stirred until to complete saporification. After then 600ml of soft water was added slowly with it and stirred until to form a jelly like substance. Then 1250 g creosote oil, 2.5 g carbolic acid and 9.5 litters of soft water was added with continuous stirring. The product thus obtained was powerful phenyl. It was applied on different bacteria to determine the Antibacterial activity and MIC value.

Source of Information: Bangladesh J. Sci. Ind. Res. 41(3-4), 203-206, 2006

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The saponification value and the α-elaeostearic acid content of the oil were found to be high and this properties of the oil makes very suitable for the preparation of powerful phenyl. The Antibacterial activity and MIC value of prepared powerful phenyl were determined by FDA method.

Knowledge Management: Journal