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Effect of Salt Stress on Seedling Growth and Ascorbic Acid Accumulation in Rice

M. S. Islam
Associate Professor
Department of Post-harvest Technology and Marketing, PSTU

Abstract/ Executive Summary:

An experiment was conducted in the Department of Biochemistry laboratory at Patuakhali Science and Technology University (PSTU), Dumki, Patuakhali. Two salinity levels which electrical conductivity (EC) was (6dS/m and 10dS/m with a control (0dS/m) were imposed in this experiment. A total of 22 rice varieties were selected and collected from Bangladesh Rice Research Institute (BRRI). Shoot length, root length, total dry matter (TDM) and root-shoot ratio (R/S ratio) at early seedling stage were affected more with higher concentration of salt (10dS/m). Only one variety BR7 was completely damaged in early seedling stage (at10dS/m) whereas BR8, BR9, BR15, BR16, BR17, BR19 and BRRIdhan28 did not survive at vegetative stage. Based on these parameters root-shoot length, R/S ratio and TDM, it may be concluded that, the rice variety BR2, BR12, BR14 and BRRIdhan47 were found more tolerant whereas the variety BR7, BR9, BR17 BRRI dhan28 BRRIdhan29 and BRRIdhan36 were susceptible at early seedling stage against salt stress. In this experiment ascorbic acid content of all varieties increases when exposed to salt stress. However, the decrease in ascorbic acid level was observed in the salt sensitive BR8, BR9, BR15, BR16, BR17, BR19, BRRIdhan28, BRRIdhan29, BRRIdhan35 and BRRIdhan36 varieties and the increased level was in salt tolerant BR1, BR14 and BRRIdhan47 varieties under salt stress.

Keywords: Ascorbic acid, Electrical conductivity, Rice variety, Salt stress, Seedling stage

Location: Department of Biochemistry laboratory at Patuakhali Science and Technology University (PSTU), Dumki, Patuakhali

Start Date:

End Date:

Program Area: Risk Management in Agriculture

Commodity: Rice

Objectives:

To observe the effect of salt stress on rice seedlings and ascorbic acid accumulation in rice.

Methodology:

To observe the effect of salt stress on rice seedlings twenty two rice varieties BR1, BR2, BR3, BR6, BR7, BR8, BR9, BR12, BR14, BR15, BR16, BR17, BR18, BR19, BR26, BRRIdhan27, BRRIdhan28, BRRIdhan29, BRRIdhan35, BRRIdhan36, BRRIdhan45 and BRRIdhan47 were collected from BRRI. Different saline conditions were developed hydroponically by added NaCl to the liquid growth media. The liquid nutrient media were prepared according to the composition reported by Hoagland and Arnon (1950). To assess the effect of salt stress on vegetative growth of rice in early seedling stage three solutions of NaCl were prepared to obtain electrical conductivities of 0, 6 and 10 dS/m. Electrical conductivity (EC) of 6 and 10 dS/m was developed by dissolving NaCl @ 3 and 6 g/L, respectively (Ramagopal, 1987). Each solution was poured into Petridishes which was lined with two layers of Whatman No. 44 filter paper so that the filter papers were adequately saturated with the solution. Fifty pre-germinated seeds of each of twenty two rice varieties were placed on the petridishes. Each salt concentration for all varieties was replicated three times. The petridishes were then incubated for ten days. After ten days roots and shoots were collected separately and subsequent data was recorded.To observe the accumulation of ascorbic acid in leaves seven days old seedlings were planted in Hoagland solution. One seedling was planted in each hole of cap of the plastic pot. Each pot accommodated three seedlings and the fourth hole was left open for adding nutrient solution or distilled water. Seedlings were fixed properly in the hole with the help of a small piece of foam. One liter Hoagland solution was taken in each pot. This volume was maintained by adding required amount of distilled water everyday. The pots were placed under sunlight. When the plants were four weeks old, the nutrient solutions in the pots were replaced by Hoagland solution containing 0 for (control) 0ds/m, 3gNaCl/L for 6ds/m and 6gNaCl/L for 10dS/m. The culture solution was renewed weekly and the pH was adjusted daily to 5.5 by adding either NaOH or HCl. Nutritional status of the media was maintained according to the method describe by Haque et al., 1996. Each treatment was done in triplicate. After plant has passed 15 days in imposed NaCl, the leaves of an individual plant were subjected to analysis for ascorbic acid content. Ascorbic acid contents of leaves were determined according to the method developed by Reo (1954).

Source of Information: J. Patuakhali Sci. and Tech. Univ. 2012, 3(2):119-127

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Considering shoot length, root length, TDM and root-shoot ratio at early seedling stage, the rice variety BR1, BR2, BR18 and BRRIdhan 47 seems to be more tolerant to salt stress compare to others. The rice variety BRRIdhan47, BR6, BR12, BR14 and BR18 contained the higher amount of ascorbic acid (1.75, 1.5, 1.25, 1.30 and 0.76 mg/100g fresh leaves) at high saline condition and the values were 84, 61, 45, 35 and 17 fold higher than that of control. At low saline condition (EC of 6 dS/m), variety BR8, BR9, BR15, BR16, BR17, BR19, BRRIdhan28, BRRIdhan29, BRIIdhan35 and BRRIdhan36 appeared to be susceptible, having an increase of 2.8, 6.2, 13, 8.2, 4.1, 10.5, 9.8, 7.5, 4.0, 4.7 fold L-ascorbic acid content over the respective control (Table 6).These susceptible varieties were damaged at EC of 10 dS/m saline stressed condition except the varieties BRRIdhan35 and BRRIdhan36. In the nonsalinized condition the seedling showed normal growth over salinized condition. Seedling growth decreased with increasing salt concentration in all the varieties.

Knowledge Management: Journal