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Development of Artificial Breeding Technique of Gutum, Lepidocephalichthys Guntea (hamilton, 1822) Using Carp Pituitary Gland

M.A. Sayeed
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh-2202

S. Akter
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh-2202

A.K. Paul
Department of Fisheries, Govt. of Bangladesh, Dhaka

M.R. Ahashan
Department of Fisheries, Govt. of Bangladesh, Dhaka

M.M.H. Miah
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh-2202

M.A.R. Hossain
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh-2202

Abstract/ Executive Summary:

A study was conducted on artificial breeding of gutum, Lepidocephalicthys guntea using carp pituitary gland. After rearing, the ready matured female fishes were injected at a rate of 20, 15 10 and 5 mg PG/kg body weight with two injections at 4 and 8, 6 and 10 and 8 and 12 PM. The female fishes were applied at first one-third and then two-third of the dose. The males were treated only with the second dose of injection. In the highest dose, all the fishes spawned naturally without stripping, but in other doses, partial natural spawning occurred that completed with hand stripping. Average fertilization rates (%) were 60.12±5.53, 62.18±2.22, 75.87± 5.66 and 78.60±3.21 and hatching rates (%) were 49.43±2.14, 50.5±4.21%, 60.32±3.43 and 65.49±5.23, respectively. Fertilization and hatching rates were significantly (P<0.05) higher in lower two doses. The incubation period of eggs observed 22-24 hrs at water temperature 28^oC, and the larvae started to feed within 72 hours after hatching. The survival rate of larvae found up to 50% and their average weight was 65 mg within two weeks of feeding with finely ground boiled chicken eggs. The successful dose for induced breeding of gutum was 5-10 mg PG/kg body weight and suitable time set of injection was 8 and 12 PM.

Keywords: Gutum, Induced breeding, Weaning, Fertilization, Survival

Location: Department of Fisheries Biology and Genetics under the Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh

Start Date: 00-04-2006

End Date: 00-09-2006

Program Area: Animal Health and Management

Commodity: Fish

Objectives:

1. To develop techniques of induced breeding and larval rearing of L. guntea.

Methodology:

Study site: The experiment was carried out at the hatchery of the Department of Fisheries Biology and Genetics under the Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh during April to September 2006. Experimental Design: Thirty-seven pairs of gutum (L. guntea) used for this experiment were collected from the upazilla Modan under Netrokona district. The average weight of male and female fish was 8.3±1.8g and 9.7±2.2g, respectively. They were reared in two tanks of the size 35×30×30 cm³ having twenty pairs and 35×25×20 cm³ having seventeen pairs. During breeding time i.e., late June, 2006 one tank was used for rearing the brood fish and another tank was used for spawning and subsequent eggs incubation purpose. Both rearing and spawning tanks were provided with aerator (DAIVO PUMP NS 8200) for the aeration of water. Brood fish rearing and maturity study: The brood fish were reared up to maturation for spawning in the hatchery by providing locally available pelleted feed to the rearing tanks. During breeding season (June-July), the body colors of the male fish were prominent with brightly reddish with yellow and greenish strip while the female fish were with comparatively less color. The abdomen of the female fish was swollen and soft. Brownish eggs would come out during gentle pressure on the abdominal region from anterior to posterior. From the above criteria the brood fish were selected for induced breeding. Application of PG to the brood fish: In this experiment 24 pairs of the brood L. guntea were injected with PG at the doses of 20, 15, 10 and 5 mg PG/kg body weight for spawning. The female fish were administered two injections whereas the male fish were injected with two-third of the dose in a single injection at the time of second injection of female fish. Reproductive activities including nudging, dozing, circling, ovulation and finally, spawning was observed in the rearing tanks and then transferred to the bowls. Time interval between two injections: Three sets of time were used viz., 4 and 8 PM; 6 and 10 PM and 8 and 12 PM (designated as T1, T2 and T3) at an interval of 4 hrs to identify the best time interval of injections. In all these treatments, the brood fish started to spawn after 8-10 hrs of second injection. After nudging and circling, the male bends its body around the female. The genital pore of male was brought nearer to the genital pore of female. Eggs were ejected, and at the same time male released the milt. Statistical analysis: Comparison of treatment means were carried out using one-way analysis of variance (ANOVA). Duncan’s Multiple Range Test (DMRT) was performed to compare the different treatment means at 5% level of significance. Statistical analysis was performed using SPSS software package.

Source of Information: J. Agrofor. Environ. 3 (1): 195-197, 2009; ISSN 1995-6983

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The lowest successful dose for the induced breeding of female gutum was 5-10 mg PG/kg body weight applying at time 8 and 12 PM at an interval of 4 hrs. between two injections. The fertilization and hatching rates of the fish were good and specific zooplankton for larval rearing should be applied for higher survival and growth of larvae by regular fertilization and manuring. Further experiments to identify specific zooplankton for larval rearing should be addressed for higher survival and growth of larvae and the successive culture of food plankton to make the culture of L. guntea as a sustainable technique is essential.

Knowledge Management: Journal