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Reproductive Biology of Feather Back, Chital (notopterus Chitala, Ham.) Cultured in a Pond of Bangladesh

A.H.M. Kohinoor
Freshwater Station, Bangladesh Fisheries Research Institute, Mymensingh, Bangladesh

D.A. Jahan
Freshwater Station, Bangladesh Fisheries Research Institute, Mymensingh, Bangladesh

M.M. Khan
Freshwater Station, Bangladesh Fisheries Research Institute, Mymensingh, Bangladesh

M.S. Islam
Freshwater Station, Bangladesh Fisheries Research Institute, Mymensingh, Bangladesh

M.G. Hussain
Freshwater Station, Bangladesh Fisheries Research Institute, Mymensingh, Bangladesh

Abstract/ Executive Summary:

Studies on Gonadosomatic index (GSI), ova diameter and histology of the gonad were performed to understand reproductive biology of Feather back, Chital (Notopterus chitala) for a period of 6 months from January to June 2010. Thirty live chital fish were used in this study. The mean GSI values for female chital were found to range between 0.20±0.013 and 4.63±0.50. The highest GSI value was found in June. The smallest diameter of ovum was recorded 0.04 mm (January) and the largest was 4.00 mm (June). During the experimental period, the fecundity was ranged from 8,238 to 18,569 (mean 13,052±4607) in fish samples with body weight range from 1,296 to 2,360 (mean 1,742.50±474.44 g) while the relative fecundity was 5.65 to 14.33. Histological study revealed that the percentage of late perinucleolus (LPN) stage was highest in April and Cortical alveoli (CA) stage appeared from April and reached to maximum in May. Vitellogenic stage (VG) appeared in the month of May and chronologically increased through June. Vitellogenic stage (VG) of oocyte as well as highest ova diameter reached to the peak in June. The variations in the gonad weight and GSI of the female fish reached to the peak during June indicating maturity of ovary and definite spawning season.

Keywords: Reproductive Biology, Feather Back, Chital

Location: Freshwater Station, Bangladesh Fisheries Research Institute, Mymensingh, Bangladesh.

Start Date: 01-01-2010

End Date: 30-06-2010

Program Area: Variety and Species

Commodity: Fish

Objectives:

To study the reproductive biology and breeding technique in captivity of chital fish.

Methodology:

Fifty brood fish of chital were stocked in a rearing pond with an area of 400 m² between August and September. After stocking, the brood fishes were fed with live food such as small fish, tilapia fry, small prawn etc. @ 2% of estimated body weight. Fish sample collection: Five female live fish samples were collected and live fish were transported to the laboratory and the gonads were removed by opening the peritoneal cavity and were used for the study. Determination of the reproductive periodicity: The methods were used to determine the reproductive periodicities of the fish are as follows: Gonadosomatic index (GSI) Total body weight of selected fishes in each month was considered to calculate the mean Gonadosomatic Index (GSI). Gonadosomatic Index (GSI) was calculated according to the formula: GSI = (Weight of gonad/Weight of fish)X 100 Diameter of ova: After dissecting the overy, small representative part from the anterior, posterior and middle portion of the ovary was removed separately. The ova of the samples were separated using physiological saline solution (0.65% NaCl) with a petridish and spread on a glass slide to measure the diameter under a microscope with an ocular micrometer. The units of the ocular micrometer that visible in the microscope were standardized with a stage micrometer for measurement of ova diameter in micrometer (μm). Approximately 300 ova from each sample were measured and ova diameters were determined and were expressed in unit value of the ocular micrometer. Histology of gonad To study the sequential histological changes, the ovaries tissues were fixed in aqueous Bouin’s fluid and then routine histological process was followed for slide preparation. The gonadal tissues were serially sectioned at a thickness of 4μ and stained with haematoxylin-eosin. From the histological preparation of the gonadal tissues, the cells and nuclear diameters of cells and various oocytes were measured by ocular micrometer.

Source of Information: ISSN: 2224-0616 Int. J. Agril. Res. Innov. & Tech. 2 (1): 26-31, June, 2012 Available online at http://www.ijarit.webs.com

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The variations in the gonad weight and gonado somatic index (GSI) of the female chital fish reached to the peak during June indicating maturity of ovary and definite spawning season. While Vitellogenic stage (VG) of oocyte as well as highest ova diameter was observed in June also. The present study on reproductive biology of chital may contribute to the seed production technology of this fish species in captivity, which may prelude to their introduction as a potential candidate for aquaculture and paves the way to conserve this endangered native fish species.

Knowledge Management: Journal