Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Insecticidal and Repellent Activities of the Chloroform Extracts of Urena Sinuata L. Against Tribolium Castaneum (herbst) Adults

M Abdullah
Department of Zoology, University of Rajshahi, Rajshahi

A Kumar Pk
Department of Zoology, University of Rajshahi, Rajshahi

D K M A Saleh
Department of Zoology, University of Rajshahi, Rajshahi

R Islam
Department of Zoology, University of Rajshahi, Rajshahi

A R Khan
Department of Zoology, University of Rajshahi, Rajshahi

N Islam
Department of Zoology, University of Rajshahi, Rajshahi

Abstract/ Executive Summary:

The insecticidal and repellent activity tests of the chloroform extracts of fruit, leaf, root and stem of Urena sinuata L. against the red flour beetle Tribolium castaneum L. adults have been conducted. The leaf, root and stem extracts showed high toxicity by giving mortality of the beetles within 30 min. of exposure. The LD₅₀ values for the 12, 24, 36 and 48h of exposures were 0.354, 0.262, 0.209 and 0.196mg cm² for the fruit extract; 0.587, 0.413, 0.355 and 0.299mg cm² for the leaf extract; 0.440, 0.389, 0.340 and 0.268mg cm²for the root extract and 0.968, 0.661, 0.491 and 0.362mg cm² for the stem extract. The insecticidal activity can be arranged in the order of fruit> root> leaf> stem extracts. The root and stem showed repellent activity against T. castaneum adults at P<0.01 and P<0.05 levels of significance, while the fruit and the leaf extracts did not show repellency at all.

Keywords: Mortality, repellency, Urena sinuata, Tribolium castaneum.

Location: Rajshahi University Campus

Start Date:

End Date:

Program Area: Pest Management

Commodity: Insecticide

Objectives:

Urena sinuata plant was taken into consideration for investigation of its bioactive potentials.

Methodology:

Fresh plants (U. sinuata) were collected from the Rajshahi University Campus, Rajshahi-6205, Bangladesh. Its identification was confirmed by Prof. ATM. Naderuzzaman, and a voucher specimen (No. 41, 02-04-2008) is also kept in the herbarium of the Department of Botany, University of Rajshahi. After collection leaves, roots and stem were separated, and were chopped into small pieces to dry in a well-ventilated room/under shade. Fruits were also collected and processed. Dried materials were then powdered in a grinder, weighed and placed in separate conical flasks to add chloroform (100 gm × 300 ml × 3 times) for 48 hours. Filtration was done by Whatman filter paper at 24h interval in the same flask followed by evaporation until the extract was left. It was then removed to glass vials and preserved in a refrigerator at 4⁰C with proper labeling. Tribolium castaneum used in the present experiment was reared in glass beakers (500 ml) in a standard mixture of whole-wheat flour with powdered dry yeast (19:1) (Park, 1962; Zyromska-Rudzka, 1966) in an incubator at 30°C ±0.5°C without light and humidity control for continuous supply of adults. For dose-mortality responses by surface film method doses of the CHCl₃ extracts of fruits, leaves, roots and stem were selected through ad hoc experiments by putting 50 mg of each of the extracts diluted separately in 1ml of solvent to apply in 50 mm Petri dishes and by increasing or decreasing the amount of extracts in repeated manner until a suitable mortality range was obtained. The doses selected for the fruit extract were 0.255, 0.191, 0.127, 0.096 and 0.064mg/cm², for the leaf extract 1.529, 0.764, 0.382, 0.191 and 0.096mg/cm², for root extract 1.019, 0.510, 0.255, 0.127, 0.064mg/cm² and for stem extract 3.822, 1.911, 0.955, 0.478 and 0.239mg/cm². Each of the five doses were diluted in 1 ml of solvent, poured into a Petri dish and allowed to dry. Ten adult beetles were released in each Petri dish, and the experiment of all the doses for each of the extracts was set in 3 replicates. The mortality of the beetles was assessed at certain intervals.

Source of Information: Univ. j. zool. Rajshahi Univ. Vol. 30, 2011 pp. 25-28 ISSN 1023-6104

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The juice of the leaves and roots of this plant is widely used in the treatment of various bowel complaints. In India the root is used as an external application for lumbago. For the control of stored product pests use of plant extract in laboratory trials is no more something new now-a-days. Insecticidal and repellent potentials of spice extracts against Tribolium castaneum adults. A perusal of the data achieved in this experiment clearly showed the presence of insecticidal properties in U. sinuata, as well as traces of repellent potential. Thus, a comprehensive phytochemical analysis of the plant for its insecticidal and repellent components, as well as the physiological studies of the active ingredients are very much to be solicited for their effective use in the future pest control and pharmaceutical endeavors.

Knowledge Management: Journal