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A Report on Aporcelaimellus Sp (nematoda: Dorylaimidae) from Cultivated Crops in Rajshahi University Campus

Nuzhat Ara
Department of Zoology, Rajshahi University, Rajshahi

Mosharrof Hossain
Department of Zoology, Rajshahi University, Rajshahi

Abstract/ Executive Summary:

100 nematode specimens under the genus Aporcelaimellus was identified from different cultivated crops. The genus is within the family Dorylaimidae, and contains robust animals of 0.8 to 3.5 mm body length. The specimen having the medium-sized body, short odontostyle, unsclerotized vulva, conical, dorsally not concave tail with distinctly separated layers of terminal cuticle. Aporcelaimellus prevalence was the highest in banana (80%) and the lowest was in mulberry (20%) during sampling periods. The diagnostic morphology of this nematode was slender vulva lips, thick vagina, and with long anal body with sharply pointed tail.

Keywords: Nematode, Aporcelaimellus, Root nematode

Location: Rajshahi University Campus

Start Date: 01-11-2006

End Date: 31-03-2007

Program Area: Pest Management

Commodity:

Objectives:

To study the prevalence and morphology of Aporcelaimellus collected from the cultivated crops in Rajshahi University Campus has been reported

Methodology:

The nematode samples were collected from cultivated crops of a particular area of Rajshahi University Campus. This study area was selected due to repeated cultivation of the same crops cultivars round the year. To accomplish this study, several crop fields were surveyed during the winter season from November, 2006 to March, 2007. Soil and root samples were collected from the study fields where infestation of nematodes were suspected considering visible symptoms described by Ayoub (1980). Samples were collected at random at depth of 5-15 cm surrounding roots and rhizospheres of crop plants. About 500 g soil along with roots was collected from each place. After collection samples were placed in airtight containers. Polythene bags were used for storing and carrying the samples to the laboratory. Then modified Baermann funnel technique was used to extract nematodes in the laboratory of the Department of Zoology, Rajshahi University according to Robinson and Heald (1989). Whole mount and microscopic slide: Three or four drops of clear fingernail polish were placed on The nematode samples were collected from cultivated crops of a particular area of Rajshahi University Campus. This study area was selected due to repeated cultivation of the same crops cultivars round the year. To accomplish this study, several crop fields were surveyed during the winter season from November, 2006 to March, 2007. Soil and root samples were collected from the study fields where infestation of nematodes were suspected considering visible symptoms described by Ayoub (1980). Samples were collected at random at depth of 5-15 cm surrounding roots and rhizospheres of crop plants. About 500 g soil along with roots was collected from each place. After collection samples were placed in airtight containers. Polythene bags were used for storing and carrying the samples to the laboratory. Then modified Baermann funnel technique was used to extract nematodes in the laboratory of the Department of Zoology, Rajshahi University according to Robinson and Heald (1989). Whole mount and microscopic slide: Three or four drops of clear fingernail polish were placed on a clean microscope slide in a triangular or rectangular pattern; it was arranged so that the cover slips will span all of them. Using the dropper, a small drop of water containing nematodes was placed in the space defined by the drops. Then carefully pass out the slide over a slow flame six to eight times to relax the nematodes and observed under the dissecting microscope to be sure that the nematodes have stopped moving. Finally nematodes were observed using a compound microscope. The morphology and some internal structures were studied and identified up to genus following taxonomic key (Andrassy, 2002).

Source of Information: Univ. j. zool. Rajshahi Univ. Vol. 30, 2011 pp. 45-48 ISSN 1023-6104

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

This paper reported a main morphological pattern of the genus is the structure of tail, by which Aporcelaimellus can be easily differentiated from every known species on the other hand. Female gonads paired, opposed, reflexed; orientation of vulva not stated for most species Nematode population density in the roots in this study could be an indicator to determine whether the crops cultivar was resistant to nematode invasion. In addition the 5-15 cm soil depth of rhizosphere should be emphasized as the important region for chemical control of this pest in the present finding.

Knowledge Management: Journal