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Assessment of in Vivo Protein Digestibility of Different Feed Ingredients Used in Rearing Silver Barb (puntius Gonionotus)

M.M. Haque
Department of Fisheries, Narsingdi

M.M. Hassan
Faculty of Fisheries, University of Stirling

M.S. Islam
Fisheries and Marine Resource Technology Discipline, Khulna University, Khulna

M.A. Haque
Department of Fisheries Management, BAU, Mymensingh,

M.M. Islam
Department of Aquaculture, BAU, Mymensingh

M.H. Rahman
Department of Aquaculture, BAU, Mymensingh

Abstract/ Executive Summary:

Apparent protein digestibility (APD) of different feed ingredients in silver barb (Puntius gonionotus) was determined at the Fish Nutrition and Fish Physiology Lab of Fisheries and Marine Resource Technology (FMRT) Discipline of Khulna University from September 2007 to January 2008 taking four ingredients and test diets using in vivo technique. Five different diets (reference and four test diets) were formulated using different feed ingredients viz. fish meal (FM), meat and bone meal (M&B), soybean meal (SM), and sesame oilcake (SOC), were designated as RD and TD₁, TD₂, TD₃ and TD₄ respectively. Reference diet contained 30.98% protein and the test diets were formulated by the 30% substitution of reference diet with respective feed ingredients. Chromic oxide (Cr2O3) was used as inert marker (0.5%) in diets. The fish were kept in aquaria (n = 30; weight 27.55±2.06 gm and length 13.25±1.34 cm) and reared on reference and different test diets to collect the faeces of the respective food and ingredients. Highest APD among the diets was obtained in TD₁ (70.11±1.47) at significant difference (P<0.05) with TD₂ (66.94±0.24), TD₃ (68.40±0.39) and TD₄ (63.33±1.09). Also significantly higher APD among the ingredients was obtained in FM (90.81±4.89%), SM (81.47±1.317) and M&B (78.55±0.80) and comparatively lower protein digestibility was obtained in SOC (59.08±3.65). From the present research it is presumed that fish meal, soybean meal and meat and bone meal would be good sources of protein for suitable diet formulation for silver barb.

Keywords: Protein digestibility, Puntius gonionotus, In-vivo technique, Feed ingredients

Location: Fish Nutrition and Fish Physiology Lab of Fisheries and Marine Resource Technology (FMRT) Discipline of Khulna University

Start Date: 00-09-2007

End Date: 00-01-2008

Program Area: Animal Health and Management

Commodity: Feed

Objectives:

1. To evaluate the in vivo protein digestibility assay of some food ingredients that can be applied to the practical evaluation of alternative protein sources for Puntius gonionotus diet preparation.

Methodology:

Study area: The present research was conducted at the Fish Nutrition and Fish Physiology Lab of Fisheries and Marine Resource Technology (FMRT) Discipline of Khulna University, Khulna from September 2007 to January 2008. Proximate analysis of different feed ingredients: Seven different types of feed ingredients viz. fishmeal (fish pack), soybean meal, sesame oilcake, maize meal, rice polishing, wheat flour and meat and bone meal were collected from local market. All the ingredients were homogenized separately by grindings. Proximate composition viz. protein and moisture of different ingredients and diets were analyzed according to AOAC Feed formulation: In the present research, five different types of diets (Reference and 4 Test diets) were formulated by using ‘Pearson Square’ method and formulated diets or pellet using hand pellet machine. The reference diet was formulated that contained 30% crude protein. Chromic oxide (Cr₂O₃) was used as an inert marker at a concentration of 0.5% in reference diet. Four test diets were formulated using a combination of 70% reference diet and 30% of the test ingredients. The test diets were designated as TD₁, TD₂, TD₃ and TD₄ for fish meal, meat & bone meal, soybean meal and sesame oilcake respectively. Fish collection and rearing: Thirty live specimens of Puntius gonionotus (weight 27.55±2.06gm and length 13.25±1.34cm) were collected from local ghar and kept in the aquaria at the Fish Physiology labs of FMRT Discipline of Khulna University. Five fish were kept in each of the six aquaria with continuous aeration. Water temperature in the aquaria was kept around 27?C. The fishes were acclimatized and reared on practical feed (30 % protein) in different aquaria for one week prior to the feeding trial started. Feeding and fecal collection: The fish of all the aquaria were acquainted and habituated with the reference diet for four days before the faecal matter was collected. Two hours after serving the food and 70% water of the aquaria was changed to remove the uneaten food and wastes. The faeces were collected at every 30 minutes intervals from each aquarium using a collection tube (dropper) for four hours. Collection of the faeces was stopped when the weight of the faeces was about to 10g. Faeces of the test diets were also collected following the same procedure after 4 days of habituation with the diets. After collection of the faeces in air tight glass bottle, it was kept at -18?C until used for further analysis. Protein content of faeces were determined Analytical methods: Chromic oxide concentration of the diets and faeces were determined. The apparent protein digestibility co-efficients (APDCs) of dry matter and protein for the test diets and ingredients were calculated. Statistical analyses: Spread sheet analysis of data was done using Microsoft Excel, version 5.0, Microsoft Inc. USA. One way single factor analysis of variance (ANOVA) followed by Duncan’s Multiple Range Test was done using SPSS 12.0 for windows.

Source of Information: J. Agrofor. Environ. 3 (2): 155-158, 2010; ISSN 1995-6983

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The fish were kept in aquaria (n = 30; weight 27.55±2.06 gm and length 13.25±1.34 cm) and reared on reference and different test diets to collect the faeces of the respective food and ingredients. Highest APD among the diets was obtained in TD₁ (70.11±1.47) at significant difference (P<0.05) with TD₂ (66.94±0.24), TD₃ (68.40±0.39) and TD₄ (63.33±1.09). Also significantly higher APD among the ingredients was obtained in FM (90.81±4.89%), SM (81.47±1.317) and M&B (78.55±0.80) and comparatively lower protein digestibility was obtained in SOC (59.08±3.65). From the present research it is presumed that fish meal, soybean meal and meat and bone meal would be good sources of protein for suitable diet formulation for silver barb.

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