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Performance of Different Strains of Shiitake Mushroom (lentinus Edodes) on Saw Dust

Nirod Chandra Sarker
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Saleh Ahmed
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Kamal Hossain
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Akhter Jahan
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Nur Hohammed Mezbahul Quddus
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Abstract/ Executive Summary:

To determine their performance nine different strains of shiitake mushroom were cultivated on sawdust. A wide variation was found in mycelium growth rate and duration to complete mycelium running. The highest mycelium growth rate (0.35cm/day) was obtained in Lentinula edodes (Le)-9, which was statistically similar to Le-6, Le-8, Le-10 and Le-4 and the lowest mycelium growth rate was obtained in Le-2. The minimum time required to complete mycelium running (35.17 days) was observed in Le-9 followed by Le-6 and Le-8 whereas maximum time required to complete mycelium running was found in Le-2. Among the nine strains, Le-10 performed best in the respect of total time required from inoculation to bump formation, primodia initiation and first harvest. Highest number of effective fruiting body (14.33/packet) was recorded in Le-8 which was statistically similar to Le-11 and the lowest number of effective fruiting body was recorded in Le-4. The highest length of stalk (5.50 cm), diameter of stalk (1.30cm), diameter of pileus (6.917cm) and thickness of pileus (1.25 cm) were observed in Le-5 which was statistically similar to Le-6. Significant variation in biological yield, economical yield and biological efficiency were observed among the selected strains. The highest biological yield, economic yield and biological efficiency were estimated in Le-8 followed by Le-11 and Le-12 whereas Le-4 performed very poorly.

Keywords: Shiitake mushroom strains, Mycelium, Fruiting body, Growth and yield.

Location: National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Start Date: 00-10-2008

End Date: 00-04-2009

Program Area: Variety and Species

Commodity: Mushroom

Objectives:

To evaluate the performance of different strains of shiitake mushroom.
Too identify the best strain that can be highly productive and suitable for culture conditions in Bangladesh.

Methodology:

The experiment was conducted at National Mushroom Development and Extension Centre, Savar, Dhaka during October 2008 to April 2009 to study the performance of vegetative and reproductive growth and yield of nine strains of shiitake mushroom. The strains were designated as Le-2, Le-4, Le-5, Le-6, Le-8, Le-9, Le-10, Le-11 and Le-12. Pure culture of different strains were prepared on potato dextrose agar (PDA) medium containing 200g peeled and sliced potato, 20g dextrose and 20g per liter agar. The medium was poured into glass Petri dishes (90mm diameter) at 15 ml/dish. The medium in Petri dish was sterilized in an autoclave for 20 minutes at 120 0C under 1 kg/cm2 pressure. After sterilization and solidification, the plates were inoculated separately with the inocula of the strains. Medium of mother culture was prepared by mixing sawdust and wheat bran at the ratio of 2:1. Calcium carbonate was added at the rate of 0.2% of the mixture. The moisture level of the mixture was maintained at 65%. Polypropylene bags of 25cm × 17 cm size were filled with 250g of the above prepared mixture and packed tightly. The neck of the bag was prepared by using heat resistant plastic pipe. A hole of about 2/3 deep of the volume of the bag was made for space to put the inoculums. The neck was plugged with cotton and covered with brown paper and tied with a rubber band. The packets were sterilized in an autoclave for one hour at 120 oC under 1 kg/cm2 pressure. Saw dust was used as a main substrate and wheat bran, rice husk were used as supplements. For each 500g spawn packet sun dried saw dust, wheat bran and rice husk were mixed together at 160, 80 and 10g respectively. Water was added to adjust moisture content at 65% and CaCO₃ was mixed at the rate of 0.2% of the mixture. Polypropylene bags of 25 cm × 17 cm size were filled with 500g of substrate mixture and packets were tied, plugged and covered as mentioned above. The packets were sterilized in an autoclave for 1 hour at 120^oC under 1 kg/cm2 pressure. Biological yield in gram per packet was recorded by weighing the whole fruiting bodies without removing the lower hard and dirty portion whereas economic yield was recorded after removing lower hard and dirty portion of fruiting bodies and biological efficiency (%) was determined by the following formula: Biological efficiency (%) = {Total biological yield (g) / Total dry substrate used (g)}× 100.The experiment was laid out in a completely randomized design with six replications (packets). Data on mycelium growth rate, duration to complete mycelium running, time required for bump formation, days required to primodia initiation, days required to first harvest, number of effective fruiting body, length of stalk, diameter of stalk, diameter of pileus, thickness of pileus, biological yield and economical yield were recorded. Data were analyzed using MSTAT-c computer program. Means were compared following Duncan’s multiple range test (DMRT) using the same computer program.

Source of Information: Bangladesh J. Mushroom. 3(1): 1-7, 2009 (June)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Among the nine strains, Le-10 performed best in the respect of total time required from inoculation to bump formation, primordia initiation and first harvest. Highest number of effective fruiting body (14.33/packet) was recorded in Le-8 which was statistically similar to Le-11 and the lowest number of effective fruiting body was recorded in Le-4. The highest length of stalk (5.50cm), diameter of stalk (1.30cm), diameter of pileus (6.917cm) and thickness of pileus (1.25cm) were observed in Le-5 which was statistically similar to Le-6. Significant variation in biological yield, economical yield and biological efficiency were observed among the selected strains. The highest biological yield, economic yield and biological efficiency were estimated in Le-8 followed by Le-11 and Le-12 whereas Le-4 performed very poorly. The highest biological yield were found in Le-8 which was followed by Le-11, Le-12, Le-10 and Le-2. The lowest yield was recorded from Le-4, which was followed by Le-9, Le-5 and Le-6. The trends in biological efficiency of different strains were the same as found in biological and economic yield. The result is in line with the report of Przybylowicz and Donoghue (1990) who found the biological efficiency of shiitake mushroom to vary between 50 and 80% for 2-5 harvests. The strain Le-4 performed very poorly.

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