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Effect of Culture Media and Environmental Factors on the Mycelial Growth of Grifola Frondosa

Jebunnahar Khandakar
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Md. Nazim Uddin
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Nirod Chadra Sarker
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Mafroz Ahmed Basunia
Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka, Bangladesh.

Md. Asaduzzaman Khan
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Abdus Salam Khan
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Abstract/ Executive Summary:

The effects of culture media, pH, temperature, light and carbon sources on the mycelial growth of Grifola frondosa were investigated. The best mycelial growth (0.3375 cm/day) was observed on potato dextrose agar medium while the least growth (0.1200 cm/day) was recorded on cane juice yeast agar medium. The optimum temperature for the best mycelial growth of this mushroom was 24⁰-27⁰C. The highest mycelial growth (0.2775 cm/day) was recorded in pH 5.0 while it was the lowest in pH 9.0. The best mycelial growth and density of mycelium were found in absence of light (0 lux). Among the five carbon sources, fructose was the best for mycelial growth of Grifola frondosa.

Keywords: Grifola frondosa, Culture media, pH, Temperature, Light, Carbon sources, Mycelial growth.

Location: National Mushroom Development and Extension Center (NAMDEC), Sobhanbag, Savar, Dhaka, Bangladesh

Start Date:

End Date:

Program Area: Food Safety and Security

Commodity: Mushroom

Objectives:

To find out the suitable culture media, nutritional requirements and environmental factors that enhance the optimum mycelial growth of the mushroom.

Methodology:

This experiment was conducted in the Tissue Culture Laboratory of National Mushroom Development and Extension Center (NAMDEC), Sobhanbag, Savar, Dhaka. The inocula of Grifola frondosa were collected from the germplasm center of NAMDEC. Eleven different culture media such as potato dextrose agar (PDA), potato dextrose yeast agar (PDYA), malt extract agar (MEA), malt extract yeast agar (MEYA), yeast extract agar (YEA), cane juice agar (CJA), Cane juice yeast agar (CJYA), wheat extract agar (WEA), wheat extract yeast agar (WEYA), Sawdust extract agar (SEA) and Corn extract agar (COEA) medium were used to determine the best medium for mycelial growth of Grifola frondosa. Constituents of different culture media were maintained. The selected PDA medium was adjusted to 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, and 9.0 pH level by adding 1N HCl or NaOH before autoclaving. To determine a suitable growth temperature the inoculated plates were incubated at bellow 8^oC, 10-13^oC, 17-23^oC, 24-27^oC, 28-31^oC, and 35^oC separately. In case of light effect, the inoculated plates were incubated at different light intensity viz: 0 lux (continuous dark), 40 to 60 lux, alternation of light (40-60 lux) and dark (0 lux),120 to 150 lux, alternation of light (120-150 lux) and dark (0 lux), 250 to 300 lux, alternation of light (250-300 lux) and dark (0 lux) as well as alternation of light (200- 280 lux) and dark (0 lux) separately at 21⁰ to 25^oC. Five different carbon sources such as glucose (pH 6.63), maltose (pH 6.65), sucrose (pH 6.68), fructose (pH 6.50) and lactose (pH 6.47) were used to investigate the mycelial growth of Grifola frondosa. Two percent of each carbon source was mixed separately with 2% agar and boiled until the agar dissolved. The experiment was laid out following completely randomized design (CRD) with 4 replications. Data on mycelium growth rate (cm/day) was recorded according to the method and formula. The collected data were analyzed and using MSTAT-C computer program. Means were separated following Duncan’s Multiple Range Test (DMRT) using the same computer program.

Source of Information: Bangladesh J. Mushroom. 3(1): 15-20, 2009 (June)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Significantly the highest mycelial growth (0.3375 cm/day) was observed in PDA medium followed by MEA which was statistically similar to YEA and WEYA. The lowest mycelial growth (0.1200 cm/day) was recorded in CJYA. MYA was the best culture medium for mycelial growth of Grifola frondosa. The highest mycelial growth (0.2325 cm/day) was found in absence of light (0 lux) which was followed by alternation of light (120-180 lux) and dark (0 lux) as well as alternation of light (250-300lux) and dark (0 lux) condition. The highest mycelial growth (0.1475 cm/day) was found in fructose which was followed by maltose, glucose and lactose.The optimum temperature for the best mycelial growth of this mushroom was 24⁰-27⁰C. The highest mycelial growth (0.2775 cm/day) was recorded in pH 5.0 while it was the lowest in pH 9.0. The best mycelial growth and density of mycelium were found in absence of light (0 lux). Among the five carbon sources, fructose was the best for mycelial growth of Grifola frondosa.

Knowledge Management: Journal