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Effects of Gamma Radiation on the Reproductive Organs in the Red Flour Beetle Tribolium Castaneum (herbst)

P. A. Banu
Department of zoology, University of Rajshahi, Rajshahi 6205, Bangladesh

I. A. Ali
Department of zoology, University of Rajshahi, Rajshahi 6205, Bangladesh

M. A. Salam
Department of zoology, University of Rajshahi, Rajshahi 6205, Bangladesh

Abstract/ Executive Summary:

Gamma radiation effects on the gonads of Tribolium castaneum were assessed by cytological techniques. Pupal treatments of 15 Gy resulted in the significant reduction of testes and ovaries leading to sterility in males and infecundity in females, respectively. The present findings thus provide an important information for designing a sterile male technique for this storage pest species.

Keywords: Gamma radiation, Tribolium castaneum, Reproductive organs

Location: Department of zoology, University of Rajshahi

Start Date:

End Date:

Program Area: Pest Management

Commodity: Insects

Objectives:

To observe the gamma irradiation effects on gonads of the red flour beetles Tribolium castaneum and the cytological changes that lead to sterility characterized by infecundity.

Methodology:

The test insects: A local strain of T. castaneum was used for the histological studies. Pupae were collected from culture maintained in an incubator at temperatures ranging from 29° to 31°C with an uncontrolled RH. They were sexed by the microscopic examination of the exogenital process of the female (Halstead, 1963). Radiation treatments: Ten 2-day old pupae of both sexes were irradiated with a dose of 15 Gy. The dose rate was 10 Gy/min from a deep-therapy radiation unit of Co installed at the Institute of Food and Radiation Biology (IFRB), Atomic Energy Research Establishment, Saver, Dhaka. After irradiations, pupae were kept in a jar containing standard food media for eclosion. Fresh adults were dissected for gonads. Adults from unirradiated pupae were used as control. Dissection and staining procedures: Beetles were dissected under dissecting microscope in a drop of Ringer’s physiological solution on a wax-fixed petridish. A pair of dissecting forceps was used to open the abdominal cavity and then the reproductive organs were taken out. The surrounding tracheoles and fat body were removed. For dissection and fixations techniques described by Anwar et al. (1971) were followed. Dissected organs were fixed in Bouin’s solution for one day that stained the specimen yellow but provided inadequate contrasts for photography (Eltringham, 1930). The specimens were washed in saturated aqueous solution of lithium carbonate for 30 minutes to remove the yellow colour. Then they were washed again in distilled water for 30 min, stained in 0.5% methylene blue chloride in distilled water for 45 min and passed gradually through 30%, 50%, 70%, 90% and 100% ethanol for 15 min each to make them histoclear. Temporary squash preparations were made of the specimens, stained with acetic orcein, mounted in DPx, and then covered with slide covers. The slides were examined with a Pantex trinocular contrast microscope for histological studies. Photographs were taken using a microscope with 25 mm film (Kodacolor 200).

Source of Information: Univ. j. zool. Rajshahi Univ. Vol. 25, 2006. pp. 11-14 ISSN 1023-6104

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It indicated that treatment with these doses was sufficient to stop the process of oogenesis in T. brevicornis adults completely. The present cytological studies on the internal reproductive organs of T. castaneum could provide important information for designing a sterile male technique for this important pest of storage products.

Knowledge Management: Journal