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Effect of Culture Media and Environmental Factors on the Mycelial Growth of Volvariella Volvacea

Mahbuba Moonmoon
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Md. Nazim Uddin
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Abdus Salam Khan
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

S.M. Ruhul Amin
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Abstract/ Executive Summary:

Studies on nutritional requirement and environmental factors were carried out on synthetic culture media to evaluate mycelial growth of Volvariella volvacea. Among the different culture media used, the best mycelial growth (0.87 cm/day) and density of mycelium of this mushroom was observed on semi-synthetic, potato dextrose agar (PDA) medium while the least growth (0.57 cm/ day) was recorded on wheat extract yeast agar (WEYA) medium. The optimum temperature that suported the best growth of this fungus was 35 ⁰C while the optimum pH was 6.5. In case of light, the best mycelial growth was found in alternation of light (200-280 lux) and dark (0 lux) condition. Among five different carbon sources, sucrose was the best for mycelial growth of V. volvacea. As nitrogen source, peptone was found best for vegetative growth of V. volvacea. No significant variation was observed between the mycelial growth in different C/N ratio (6:1, 12:1, 18:1, 24:1, 36:1, 48:1 and 96:1).

Keywords: Volvariella volvacea, Culture media, Environmental factors, C/N ratio and Mycelial growth.

Location: Tissue culture laboratory of National Mushroom Development and Extension Center, Sobhanbag, Savar, Dhaka

Start Date: 00-07-2008

End Date: 00-10-2008

Program Area: Crop-Soil-Water Management

Commodity: Mushroom

Objectives:

To find out useful information about the culture media, nutritional requirements and environmental factors that enhance the optimum growth and yield of Volvariella volvacea, commonly cultivated in Bangladesh.

Methodology:

This experiment was conducted in theperi tissue culture laboratory of National Mushroom Development and Extension Center, Sobhanbag, Savar, Dhaka during July to October, 2008. The inocula of Volvariella volvacea were used from the germplasm center of NAMDEC. The inocula used in different exments were performed with 4 replications. The media was poured into petri plates at 20 ml/plates after adjusted pH 6.5. The media were autoclaved for 20 minutes at 121 ^oC temperature and 1kg/cm²pressure and cooled at normal temperature. Then same size of inocula was transferred from culture grown on PDA to placed in the centre of petri plates of each media. Then the inoculated petri dishes were transferred into incubation room for mycelial growth at 210 to 25 ^oC temperaure. The pH ranges were 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, and 9.0. After autoclaving, the petri plates were inoculated and transferred into incubation room for mycelial growth at 210 to 25 ^oC temperaure. The medium was adjusted at pH 6.5. Therefore, the medium dispensed into Petri- plates, allowed to solidify, autoclaved and inoculated with a 0.6 cm (diameter) disc of vigorously growing culture of Volvariella volvacea and incubated at 12-15 ^oC, 19-22 ^oC, 24-27 ^oC, 28-31 ^oC, 35 and 40 ^oC separately. The basal component of each media were mixed with agar. The mixtures were boiled to dissolved the agar, poured into petri plates, autoclaved, cooled at normal temperature, inoculated by V. volvacea and incubated as mentioned before. The experiment was laid out following by completely randomized design with 4 replications. Data on mycelium growth rate (cm/day), days to complete mycelium running (days) were recorded and analyzed following Gomez and Gomez, 1984 and using MSTAT-C computer program. Means were computed following DMRT using the same computer program. The following formula was used to calculate the mycelial growth.

Source of Information: Bangladesh J. Mushroom. 2(2): 33-42, 2008 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Among five different carbon sources, sucrose was the best for mycelial growth of V. volvacea. As nitrogen source, peptone was found best for vegetative growth of V. volvacea. No significant variation was observed between the mycelial growth in different C/N ratio (6:1, 12:1, 18:1, 24:1, 36:1, 48:1 and 96:1).

Knowledge Management: Journal