Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Study of Mycelial Growth of Cordyceps Sinensis in Different Media, at Different Ph Level and Temperature

S.M. Ruhul Amin
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Nadia Alam
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Mousumi Tania
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Md. Asaduzzaman Khan
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Abstract/ Executive Summary:

In this research work, four growth media: potato dextrose agar (PDA), potato dextrose yeast agar (PDYA), malt extract agar (MEA) and yeast extract agar (YEA) were used for the production of mycelium of Cordyceps sinensis. Mycelium growth of Cordyceps sinensis was the best in PDA media followed by MEA and PDYA media respectively, while it was the lowest for YEA media. To screen out a suitable pH level for the mycelial growth, PDA media was adjusted at different pH level (4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5 and 10.0) and it was found that alkaline media (8.5-9.5) was better for the mycelial growth of Cordyceps sinensis, although the variation of growth at different pH levels was not significant and mycelial growth occurs at a wide range of pH. To screen out a suitable temperature for the mycelial growth, culture medium was incubated at different temperatures (≤ 10ºC, 15ºC, 20ºC, 25ºC, 30ºC and 35ºC) and it was found that mycelium growth of Cordyceps sinensis occured at 10-25 ºC and it was best for 20-25 ºC, while no mycelial growth was found at 30 ºC and 35 ºC.

Keywords: Cordyceps sinensis, Mycelium, Media, Temperature and pH.

Location: Tissue culture laboratory of National Mushroom Development and Extension Centre

Start Date: 00-08-2008

End Date: 00-10-2008

Program Area: Socio-economic and Policy

Commodity: Mushroom

Objectives:

To determine the suitable growth medium for the production of quality mycelium within short spam of time in the climatic condition of Bangladesh.

Methodology:

This experiment was conducted in the tissue culture laboratory of National Mushroom Development and Extension Centre (NAMDEC) during August to October, 2008. Different synthetic media such as Potato Dextrose Agar (PDA), Yeast Extract Agar (YEA), Malt Extract Agar (MEA) and Potato Dextrose Yeast Agar (PDYA) were used for the study of the mycelial growth of Cordyceps sinensis . The inoculum was collected from the germplasm centre of NAMDEC. The basal component of each medium was prepared mixing 20g of dextrose and 18g of agar at neutral pH. The mixture was boiled on gas burner until the agar dissolved completely. The media was poured into petri dishes at 20 ml/plates and sterilized in an autoclave for 20 minutes at 120°C under 1 kg/cm 2 pressure. After sterilization and solidification, the plates were inoculated with the inoculum of Cordyceps sinensis . Then plates were transferred to the incubation room for mycelium growth at 25±2 ^oC temperature. To screen out a suitable temperature for the mycelial growth, culture was incubated at different temperatures. The growth medium was adjusted to the native pH of the medium (6.5 for PDA) and incubated at ≤10ºC, 15ºC, 20ºC, 25ºC, 30ºC and 35ºC separately. Radial growth of mycelia on each Petri dish was measured at 4 cross-directions. To screen out a suitable pH for the mycelial growth, media were adjusted at different pH (4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5 and 10.0) and inoculated with the inoculum of Cordyceps sinensis and incubated. Radial growth of mycelia on each Petri dish was measured at 4 cross-directions.

Source of Information: Bangladesh J. Mushroom. 2(2): 43-48, 2008 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

To screen out a suitable temperature for the mycelial growth, culture medium was incubated at different temperatures (≤ 10ºC, 15ºC, 20ºC, 25ºC, 30ºC and 35ºC) and it was found that mycelium growth of Cordyceps sinensis occured at 10-25 ºC and it was best for 20-25 ºC, while no mycelial growth was found at 30 ºC and 35 ºC.

Knowledge Management: Journal