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Grain Yield and Protein Content of Transplant Aman Rice as Influenced by Variety and Rate of Nitrogen

M.S. Hossain
Department of Agronomy, BAU, Mymensingh, Bangladesh

F.I. Monshi
Department of Biotechnology, BAU, Mymensingh, Bangladesh

A.M.A. Kamal
Department of Agronomy, BAU, Mymensingh, Bangladesh

M.F. Miah
Department of Biotechnology, BAU, Mymensingh, Bangladesh

Abstract/ Executive Summary:

An experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh, during the period from June to December 2006 with a view to studying grain yield and protein content of transplant aman rice as influenced by variety and rate of nitrogen. The experiment consisted of five varieties viz. BRRI dhan30, BRRI dhan32, BRRI dhan34, BRRI dhan39 and Nizersail and four rates of nitrogen viz. 0, 40, 80 and 120 kg ha^-1. The longest plant was found in Nizersail (146.9 cm) and BRRI dhan34 (145.2 cm) and the shortest in BRRI dhan39 (107.2 cm). As for nitrogen, the longest plant (131.7 cm) was obtained from 80 kg N ha^-1, which was identical with 120 kg N ha^-1. Variety showed significant effect on all the yield contributing characters and yield except panicle length. Results revealed that BRRI dhan39 produced the highest grain yield (4.18 t ha^-1) from BRRI Dhan34; whereas BRRI dhan30 produced the lowest grain yield (3.85 t ha^-1). BRRI dhan34 had the highest grain protein content (9.18%), identically followed by BRRI dhan39 (8.53%). All the characters were significantly influenced by rate of nitrogen except panicle length and weight of 1000 grains. Grain protein content was highest from 80 kg N ha^-1. The effect of interaction between variety and rate of nitrogen showed that the highest grain yield was obtained from BRRI dhan39 (4.90 t ha^-1) with 120 kg N ha^-1. Results showed that the variety BRRI dhan39 produced the highest grain yield and 80 kg N ha^-1 appeared to be the best nitrogen rate in respect of yield and grain protein content of rice.

Keywords: Grain yield, Protein content, T aman rice, Variety, Nitrogen

Location: Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh

Start Date: 00-06-2006

End Date: 00-12-2006

Program Area: Quality and Nutrition

Commodity: Rice

Objectives:

1. To study the yield and protein content of transplant aman rice as influenced by variety and rate of nitrogen at the Agronomy Field Laboratory, Bangladesh Agricultural University (BAU), Mymensingh.

Methodology:

The experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University (BAU), Mymensingh during the period of June to December 2006 to studying grain yield and protein content of transplant aman rice as influenced by variety and rate of nitrogen. The experiment consisted of five varieties viz. BRRI dhan30, BRRI dhan32, BRRI dhan34, BRRI dhan39 and Nizersail and four rates of nitrogen viz. 0, 40, 80 and 120 kg ha^-1. The experiment was laid out in a split-plot design with three replications. The size of unit plot was 4.0 m × 2.5 m. There were 60 plots in the experiment. The land was fertilized with triple superphosphate, muriate of potash, gypsum and zinc sulphate at the rate of 80, 60, 50, and 10 kg ha^-1, respectively. Intercultural operations and proper management practices were done. Data were recorded on plant height, no. of total tillers hill^-1, no. of effective tillers hill^-1, no. of total spikelets panicle^-1, no. of grains panicle^-1, wt. of 1000 grains, grain yield, biological yield and grain protein content. The analysis of variance was done with the help of computer package program MSTAT-C and the mean differences were adjudged by Duncan’s New Multiple Range Test. For the determination of grain protein content, the grain samples were collected from each plot and dried in an oven at 65⁰C for 24 hours after which they were ground by a grinding mill. The ground samples were sieved through a 20 mesh sieve. Approximately 10 g grain samples were preserved in polythene bags for chemical analysis to determine total nitrogen content. Chemical analysis was done by Micro-kjeldahl method (AOAC, 1984) at the Laboratory of Biochemistry Department in Bangladesh Agricultural University, Mymensingh. One gram sample was digested in 25 ml concentrated H₂SO₄ in presence of 12-15 g of mixture (CuSO₄: K₂SO₄=1:5) in a Kjeldahl flask. After the completion of digestion, the flask was cooled, 150 ml distilled water was added, followed by the addition of some glass beads and zinc granules to avoid bumping. One hundred ml of 40% NaOH solution was then introduced into the flask along the side of the flask carefully avoiding mixing and was immediately distilled. The distillate was collected in 2% boric acid solution in presence of mixed indicator in an Erlenmeyer flask. The distillate was titrated with0.2 N HCl solution and the amount of nitrogen was found in the sample was multiplied by 5.95 to obtain total protein present in the sample. The result was expressed in percentage of grain protein content calculated

Source of Information: J. Agrofor. Environ. 3 (2): 235-238, 2010; ISSN 1995-6983

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The highest grain protein content (9.18%) was observed in BRRI dhan34, which was statistically identical with BRRI dhan39 (8.53%). The lowest grain protein content (7.41%) was observed in Nizersail, which was similar to BRRI dhan32 (7.90%) and BRRI dhan30 (8.21%). In effect of nitrogen rate, the highest grain protein content (9.58%) was recorded with 80 kg N ha^-1 (N₂) and the lowest grain protein content (6.65%) was observed in the control treatment. It is reported that grain protein content of rice increased with increased rate of nitrogen application. The effect of interaction between variety and rates of nitrogen application was not significant in respect of grain protein content of rice. Numerically, the highest grain protein content (10.98%) was recorded in BRRI dhan34 with 80 kg N ha^-1 and the lowest (6.05%) was observed in Nizersail with control. BRRI dhan39 was the highest yielding variety followed by BRRI dhan32, BRRI dhan30, BRRI dhan34 and Nizersail. Nitrogen fertilization of all the varieties with 80 kg N ha^-1 appeared to be the best nitrogen rate for grain yield, straw yield and grain protein content, which were statistically similar to 120 kg N ha^-1. The highest grain yield and straw yield were produced by the combination of BRRI dhan39 with 120 kg N ha^-1.

Knowledge Management: Journal