H. ASHRAFUJZZAMAN
Department of Plant Pathology, BAU, Mymensingh
I, HOSSAIN
Department of Plant Pathology, BAU, Mymensingh
Antifungal activity, Plant extracts, Control, Rhizoctonia soleni, Bipolaris sorokiniene
Department of Plant Pathology, BAU, Mymensingh
Development of Host and Medicinal Plants
Medicinal Plants
The effectiveness of the leaf extract of 40 plants were evaluated against Rhizoctonia solani. The plants isolated from diseased rice plant used were Dolichos teolsb, Colocasia esculerne, Morus indica, Dryoplenis fitttxmas, Plstia stretiotes, Amerentbus spinosus Sesemum indicum, Curcuma zedoerie, Crotalaria [uncle, Polygonnm hyd- toplper, Solanum melonqene, Riclnus CR- mmunis, Hibuscus mutstulis, l.ewsonie alba, Ficus ntspids, Melia ezedtrecte, Allium setivum, Opuntie diltenli, Cajanus cejen, Zingiber officinalis, Allium cepe, eresstcs otersces vet. cau/if/ora, Olea europes, Eugenia jambolana, Temsrlndus indies, Aegle margelos, So/anum tuberosum, Sa/mati malalaria, Cassia ttstute, Curcuma domestics, Hydrocotyl esletlce, Capsicum Irutes cens, Duranta Ptumeirl, tycopersics escutentum, Daucus csrrote, Memor- 189 dice cbsrsntis, Portuleca olerecee, Solanum cardinense, Chenopodium album and Canna indica. Leaves of the plants were air dried and powdered. The powdered sample (1g) was added to 10 ml water and 15 ml acetone-alcohol mixture and kept for 72 hrs. under air-tight condition. Then acetone-alcohol was removed from the mixture by drying out. The mixture was kept in refrigerator until further use. The effectiveness of the extracts were evaluated by two methods: (1) Cup method-PDA plates of about 15 ml were prepared. After solidification of the medium, three 7 mm discs were cut out from three places by disc cutter. Three drops of plant extract were put into each hole and the plate was kept overnight in refrigerator in order to allow the extract to diffuse around the hole. In the following day, 7 mm fungal culture disc was placed in each hole. (2) Dise method- A 7 mm culture disc was dipped into extract for 5 minutes and then placed at the centre of plate containing about 15 ml PDA. For each extract three replication used. The radial mycelial growth as well as the formation of scleratia were recorded after 5 days of incubation. In both tests, proper control was maintained using liquid (acetone-alcohol) The efficiency of the extracts were evaluated following spore germination technique. Spore suspension of one ml having 6 x 104 spores/ml was added to 100 ml of plant extract of each concentration. For control treatment only water was used instead of extract. To enhance the conidial germination, 1 g dextrose pre 100 ml of solution was added to test the extract. The experiment was conducted at 25± 10C. The conidial germination as weir as myceliaI growth were recorded at every 12 hrs upto 5 days of incubation.
Proc. BAU Res. Prog. 6: 188-192, 1992
Report/Proceedings