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Performance of Six Strains of Reishi Mushroom (ganoderma Lucidum) on Different Amounts of Substrate

A. J. Kakon
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

Kamal Hossain
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

Nirod Chandra Sarker
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

Mahbuba Moonmoon
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

Saleh Ahmed
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

Abstract/ Executive Summary:

Six strains of reishi mushroom (Gl₁, Gl₂, Gl₃, Gl₄, Gl₅ and Gl₆) were cultured on three different amounts of substrate (500g, 750g and 1000g in wet basis) to evaluate their growth and yield performance. Among the strains, remarkable variation was not found in mycelium growth and time required to antler initiation and conk formation. Days required to complete mycelium running and mushroom harvest were increased with the increases of amount of substrate. Maximum length and diameter of stalk as well as thickness and diameter of pileus was recorded in Gl₄. The highest biological yield (36.00g/packet) was obtained from Gl₄ when cultured on 750g substrate followed by the same strain on 1000g substrate (34.50g/packet). The lowest biological yield (20.00g/packet) was recorded in Gl₃ when cultured on 500g substrate followed by Gl₅, Gl₆, Gl₁ and Gl₂ on 500g substrate. The highest biological efficiency (13.00%) was found in Gl₄ when 500g substrate was used and the least biological efficiency was exhibited in Gl₃ when cultured on 1000g substrate.

Keywords: Strains of reishi mushroom, Amount of substrate, Growth and yield.

Location: National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

Start Date: 00-04-2009

End Date: 00-08-2009

Program Area: Crop-Soil-Water Management

Commodity: Mushroom

Objectives:

To study the growth and yield performances of six strains of reishi mushroom available in Bangladesh and to standardize the amount of sawdust substrate for their cultivation.

Methodology:

The experiment was conducted at the National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka during the months of April to August, 2009. Six strains of reishi mushroom such as Gl₁, Gl₂, Gl₃, Gl₄, Gl₅ and Gl₆ and three different amounts of substrate viz. T₁=500g, T₂=750g and T₃=1000g in wet basis were used as treatment. The substrate of spawn packets were prepared by using sawdust and wheat bran at the ratio of 2:1 (dry basis). Water was added to make the moisture content 60% and CaCO₃ was added at the rate of 0.2% of the total mixture. Different sizes of polypropylene bags were filled with prepared substrate followed by above three amounts. After filling the bags, the mouths of the bags were plugged by inserting water absorbing cotton with the help of plastic neck and autoclaved at 121⁰C and 1.0 kg/cm2 for 2 hours. After autoclaving and cooling, the bags were inoculated separately with the mother culture of six strains of reishi mushroom. After inoculation, the packets were incubated in the laboratory at about 25±2 ⁰C temperatures. In incubation period, whitish mycelia begin to grow on the substrate. The fully colonized packets were used for cultivation. After completion of mycelium running, spawn packets were opened by square sized (1×1 cm) cut on the single side middle abdomen of the packet and transferred to the culture room at 25-32⁰C temperature and 80-90% relative humidity. Sufficient water was applied per day and proper aeration was maintained in culture house to develop the fruiting body. Biological yield in g/packet was recorded by weighing the whole fruiting bodies and biological efficiency was determined by the following formula: Biological efficiency (%) = {Total biological yield (g)/ Total dry substrate used (g)} × 100. The experiment was laid out following completely randomized design (CRD) with 4 replications. Data on mycelium growth rate, days required to complete mycelium running, antler initiation, conk formation and first harvest, number of fruiting body, length of stem, diameter of stem, diameter of pileus, thickness of pileus, biological yield and biological efficiency were recorded and analyzed using MSTAT-c computer program. Means separation were computed following Duncan’s Multiple Range Test (DMRT) using the same computer program.

Source of Information: Bangladesh J. Mushroom. 3(2): 33-38, 2009 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Maximum length and diameter of stalk as well as thickness and diameter of pileus was recorded in Gl₄. The highest biological yield (36.00g/packet) was obtained from Gl₄when cultured on 750g substrate followed by the same strain on 1000g substrate (34.50g/packet). The lowest biological yield (20.00g/packet) was recorded in Gl₃ when cultured on 500 g substrate followed by Gl₅, Gl₆, Gl₁ and Gl₂ on 500g substrate. The highest biological efficiency (13.00%) was found in Gl₄ when 500g substrate was used and the least biological efficiency was exhibited in Gl₃ when cultured on 1000g substrate.

Knowledge Management: Journal