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Histopathological Studies of Eus Affected Shing, Heteropneustes Fossilis (bloch) from a Fish Farm of Mymensingh, Bangladesh

M.A. Hasan
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh

K. Mostafa
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh

M. Tarikul Islam
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh

M.A. Alam
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh

M. Mamunur Rashid
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

An investigation was carried out to observe histological changes in liver and kidney of suspected epizootic ulcerative syndrome (EUS) affected shing fish Heteropneustes fossilis (Bloch) collected from the Agro-3 farm situated at Boilor, Trishal, Mymensingh. Focal necrosis, hemorrhages and atrophy of the sinusoidal region were observed in the liver tissue. Fungal granulomas were found both in liver and kidney. In some cases fatty depositions were observed in all over the hepatic tissue. Degeneration and necrosis of renal tubular epithelial cells were also occurred. Missing of glomerulus and necrosis surrounding the Bowmans capsule in the kidney tissue were found.

Keywords: Heteropneustes fossilis, EUS, Histopathology

Location: Fish disease laboratory, Department of Aquaculture, Bangladesh Agricultural University, Mymensingh

Start Date: 00-09-2006

End Date: 00-01-2007

Program Area: Animal Health and Management

Commodity: Cat fish

Objectives:

The present study deals with the histopathological findings of the fish.

Methodology:

Study area and duration - A fish farm namely Agro-3 Fish Farm was selected for the present experiment that was situated at Boilor, Trishal, Mymensingh. Duration of the study was September, 2006 to January, 2007. Collection of sample - A total of 20 Heteropneustes fossilis (Shing) were selected as the experiment fish. They were collected from one of the pond of the above farm, in which the fish were suffering from suspected EUS like lesions and were having severe mortality. Such diseased fish were caught by using seine net during sampling. No dead fish were taken. After netting, the fishes were taken in a plastic bucket with pond water and immediately brought to the fish disease laboratory, BAU, for study. Fixation and preservation - Samples of liver and kidney were collected from each fish by a sharp scalpel and forceps for histopathological study. 1 cm³ portions of each organ, from each fish were taken and fixed in 10% neutral buffered formalin. The amount of fixative was 10 times to buck of tissue fixed. After at least 10 days of fixation, the samples were trimmed for histopathological preparations. Histopathological procedure - The fixed samples were taken out with forceps from the vial and placed separately in a perforated plastic holder was covered by perforated still plates. Marking was done with dark pencil (2B) in plastic holder. The samples (blocks) were then arranged in a still rack and placed in an automatic tissue processor for dehydration, clearing and infiltration. Alcoholic ser concentrations, xylene and paraffin wax were used in the processor maintaining at various time schedules. After tissue processing (21 hours), the samples were embedded with melted wax on perforated plastic holder and still mold. The solid blocks were placed in a refrigerator (deep freeze) for half an hour and paraffin blocks were separated from the still molds. Surface and side of paraffin blocks were trimmed with the help of scalpel and microtome. Blocks having hard tissue before sectioning were decalcified by dissolving the surface of blocks in water for one hour followed by washing rapidly. The blocks were placed again in deep freeze for 30 minutes. Sections were taken from the blocks at a thickness of 5 micrometers. The ribbon with section was placed on a water bath at a temperature of 40ºC, which were finally picked up over glass slides. The sections were then stained with haematoxylene and eosin and were mounted with Canada balsam. The stained slides were done then examined under a compound microscope (Olympus). Photomicrographs were done by using a photomicroscope.

Source of Information: Bangladesh J. Fish. Res., 12(1): 21-26, 2008

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Fatty depositions were observed in all over the hepatic tissue.
Degeneration and necrosis of renal tubular epithelial cells were also occurred.
Missing of glomerulus and necrosis surrounding the Bowmans capsule in the kidney tissue were found.

Knowledge Management: Journal