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Effect of Pretreated Sawdust and Pasteurized Straw with Various Combinations on Yield of Oyster Mushroom (pleurotus Ostreatus)

Nirod Chandra Sarker
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka

A. J. Kakon
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka

Mahbuba Moonmoon
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka

Abdus Salam Khan
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka

Tahera Binte Mujib
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka

Md. Mominul Haque
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka

Taufiqur Rahman
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka

Abstract/ Executive Summary:

Different amount of pasteurized rice straw and pretreated sawdust used for cultivation of oyster mushroom and the yield as well as yield related attributes were compared. The minimum days (6.50) required to pinhead initiation (DRPI) of oyster mushroom was recorded in autoclaved sawdust with pasteurized straw (1:1) while the maximum DRPI (13.00) was recorded in without autoclaved mixture of spawn packet with pasteurized straw. The minimum days (14.00) required to final harvesting was found in without autoclaved sawdust with pasteurized straw at the ratio of 1:2 and the maximum (35.00) was found in autoclaved sawdust with pasteurized straw. The number of effective fruiting bodies was the highest (21.00) in autoclaved sawdust with pasteurized straw (1:2) and it was the lowest (7.50) in autoclaved sawdust with pasteurized straw (1:1). The length of stalk ranged from 1.80 to 2.57cm. The highest length of stalk (2.57 cm) was found in without autoclaved mixture of spawn packet with pasteurized straw (1:2) and the lowest length (1.80 cm) of stalk was found in sundry sawdust with pasteurized straw (1:1) The diameter of stalk, pileus and thickness of pileus ranged from 0.70 to 1.11 cm; 5.66 to 7.44 cm and 0.47 to 0.55 cm respectively. The highest diameter of stalk (1.11 cm) and pileus (7.44 cm) were found in sundry sawdust with pasteurized straw (1:2). The highest yield and biological efficiency was 179.80g and 89.90% respectively, in autoclaved sawdust with pasteurized straw at 1:2 ratio respectively.

Keywords: Pasteurization, Sun dry, Autoclave, Ratio, Sawdust, Straw.

Location: National Mushroom Development and Extention Centre, Sobhanbag, Savar, Dhaka, Bangladesh

Start Date: 01-01-2011

End Date: 31-03-2011

Program Area: Crop-Soil-Water Management

Commodity: Mushroom

Objectives:

To find out the easiest, economical and practicable methodology of pretreatment and preparation of substrate, which may also be helpful to increase the growth and productivity of oyster mushroom.

Methodology:

In this experiment rice straw and sawdust were used as substrate for the cultivation of Pleurotus ostreatus mushroom. Different amount of pasteurized straw and treated sawdust were used as treatments. The treatments were T₁= Sun dry sawdust + pasteurized straw (1:1) T₂= Sun dry sawdust + pasteurized straw (1:2) T₃= Autoclaved sawdust + pasteurized straw(1:1) T₄= Autoclaved sawdust + pasteurized straw (1:2) T₅ = Without autoclaved sawdust + pasteurized straw (1:1), T₆= Without autoclaved sawdust + pasteurized straw (1:2), T₇ = Mixture for commercial spawn packet (without autoclaved) +pasteurized straw (1:1) T₈ = Mixture for commercial spawn packet (without autoclaved)+ pasteurized straw (1:2). The straw was chopped to 4-5 cm length and then poured in water at 60°C for one hour and then drained out the water and kept it to get cool slowly. The polypropylene bags of 9" x 12" or 23 x 30 cm size was filled with pasteurized straw and treated sawdust according to treatments. After harvesting of mushroom, the residues were removed from the packet and temperature and relative humidity were maintained as before. The yield was obtained from single, double and third flush in the harvest period. Yield in g/packet was recorded by weighing all the fruiting bodies in a packet after removing the lower dirty portion. Biological efficiency was calculated according to the formula: Biological efficiency (%) = {Total biological yield (g) / Total dry substrate used (g)} x 100. The experiment was laid out following completely randomized design (CRD) with 4 replications. Data on days required to pinhead initiation, , number of fruiting bodies, number of effective fruiting bodies,length of stalk, diameter of stalk, diameter of pileus, thickness of pileus, days required to harvesting, yield, number of flush and biological efficiency were recorded and analyzed following Gomez and Gomez (1984) using MSTAT-C computer program. Means separation were computed following Duncan’s Multiple Range Test (DMRT) using the same computer program.

Source of Information: Bangladesh J. Mushroom. 5(2): 39-45, 2011 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The results were varied with the findings DRPI for oyster mushroom ranged 3- 4 days. This might be attributed to different environmental factors and culture substrates. The minimum days (14.00) required from opening to final harvest were observed in T6 which was statistically similar to T₇ because single flush counted. After first harvest, the packets were contaminated due to several causes. Kurtzman (2010) reported several causes of mushroom substrate contamination. There was a significant difference in percent contamination rate, which ranged from 25 to 100% by green mould (Trichoderma harzianum) and other bacteria during cultivation. The highest contamination rate (100%) was found in T₁ followed by T₅, T₆, T₇, T₈ (100%) and the lowest contamination rate was found in T₄ (25%) during third harvest. The ability of sterilization method to eliminate substrate contaminants is shown by the presence or absence of contaminants in the substrate after sterilization, spawning and incubation.

Knowledge Management: Journal