Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Isolation, Identification and Molecular Characterization of Contagious Ecthyma Virus from Goat and Sheep

Mohammed Giasuddin
Principal Scientific Officer
Animal Health Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341

MJFA Taimur
Animal Health Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341

M. Ershaduzzaman
Animal Health Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341

M S R Khan
Bangladesh Agricultural University, Mymensingh, Bangladesh

M. B. Rahman
Bangladesh Agricultural University, Mymensingh, Bangladesh

M. H-Al-Faruque
Animal Health Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341

Md. A Samad
Animal Health Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341

P Monoura
Animal Health Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341

J Alam
Chief Scientific Officer
National Institute of Biotechnblogy

M. S Alam
Animal Health Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341

Abstract/ Executive Summary:

Present study was aimed at isolation, identification and molecular characteization of the CEV circulating in Bangladesh. A total of 13 samples (scab materials; 1 1 from goat and 2 from sheep) were collected from three different farms of Sylhet and Dhaka divisions followed by processing for virus detection by PCR and isolation in Vero cell. Four different sets of primers were used targeting four different genes of CEV. Of these samples, 10 (8 from goat and 2 from sheep) were found positive by PCR. From these positive samples three viruses were isolated. Sequencing of different genes of several CEV isolates has been done directly from PCR product using automated DNA sequencer 13130 Genetic Analyzer available at the National Reference Laboratory for Avian Influenza (NRL-AI), Bangladesh. Bangladeshi isolates were found to have 95-99o/o identity to each other. When compared to viruses from other countries, high homology (92-96%) was observed to viruses from New Zealand, and India, and phylogenetically clustered with them. This is the frst report of molecular charactenzation, though partial, of CEV in Bangladesh.

Keywords: Contagious Ecthyma Virus, Detection, PCR, Molecular Characterization

Location: Bangladesh Livestock Research Institute

Start Date: 01-01-2009

End Date: 31-12-2011

Program Area: Animal Health and Management

Commodity: Goat, Sheep

Objectives:

To isolate, identify CEV from suspected field samples of goat and sheep and to characterize the viruses.

Methodology:

Sample collection and inoculam preparation: A total of 13 scab materials were collected from contagiotrs ecthyma suspected goat (n:11) and sheep (n:2) of three different farms of Dhaka and Sylhet divisions with the history of papular lesions in oral commissure, muzzle and lower jaw regions. DNA extraction: DNA was extracted from samples using Wizard Genomic DNA Purification Kit (Promega, USA) according to manufacturer's instruction. Primer: A total of four sets of primers targeting foour different genes of CEV were used to detect the CEV in the test sample. Polymerase chain reaction (PCR): PCR was performed using PCR Reagent System (Invitrogen, USA). Virus isolation and identification: Virus isolation from inoculumn prepared from scab materials was performed in Vero cell. Sequencing: PRC product was purified using EZ-10 Spin Column PCR Products Purification Kits- 85363 (Bio Basic Inc, Canada) according to manufacturer's instruction and stored at -20⁰C. Phylogenetic analysis: Multiple sequence alignments and processing were performed with theMolecular Evolutionary Genetics Analysis (MEGA) version 4.1.0 softwne with an engine based on the ClustalW algorithm.

Source of Information: Bang. J. Livs, Res. Vol. 19 (l & 2):84-90,2012; ISSN 1022-3851

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

CEV viruses were found to circulating in Bangladesh. Young goats and sheep were found more susceptible to infection. Of the 13 samples, 10 were found positive for CEV by PCR. Based on partial nucleotide sequence data generated through this study, viruses were found to be closely related to each other. Most closely related viruses were found to be derived from India and New Zealand and phylogenetically Bangladeshi isolates were clustered with them.

Knowledge Management: Journal