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Performance of Different Strains of Pleurotus Cystidiosus Under Bangladesh Condition

Kysun Rafat Howlader
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

Abdus Salam Khan
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

Mahbuba Moonmoon
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

A. J. Kakon
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

Saleh Ahmed
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

Nirod Chandra Sarker
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

Abstract/ Executive Summary:

Performance of different strains of Pleurotus cystidiosus (Pcys) were studied in National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh. Significant variation was observed in the growth, yield and yield contributing characters of these strains. The highest mycelial growth (0.58 cm/day) was observed in Pcys-4 which was followed by Pcys-5, Pcys-2 and Pcys-1. The lowest mycelial growth (0.22 cm/day) was observed in Pcys- 3. The highest biological and economic yield, 196.3 and 189.0 g/packet respectively were obtained from Pcys-1, which followed by Pcys-2, Pcys-4 and Pcys-5. The lowest biological and economic yields were observed in Pcys-6. The number of effective fruiting bodies was the highest (37.25) in pcys-1, while, the weight of individual fruiting body was the highest (26.88 g) in Pcys-6. The highest length of stipe (4.95cm) and thickness of pileus (1.35cm) were observed in Pcys-1. The strain also showed the highest biological efficiency (BE) among the strains.

Keywords: Pleurotus cystidiosus, Growth, Yield attributes, Yield.

Location: National Mushroom Development and Extension centre (NAMDEC), Sobhanbag, Savar, Dhaka

Start Date: 01-11-2010

End Date: 28-02-2011

Program Area: Variety and Species

Commodity: Mushroom

Objectives:

To investigate the performance of different strains of Pleurotus cystidiosus in order to identify the best strain that can be highly productive and suitable for the cultivation in the winter in Bangladesh.

Methodology:

Six different strains of Pleurotus cystidiosus (Pcys) available in NAMDEC were selected for the experiment. The strains were designated as Pcys-1, Pcys-2, Pcys-3, Pcys-4, Pcys-5 and Pcys-6. Pure cultures of selected strains were grown on potato dextrose agar (PDA) medium. The medium was poured into test-tube. The medium in test-tube was autoclaved at 120⁰C and 1 kg/cm² for 20 minutes. After sterilization and solidification, the test-tubes were inoculated with the inocula of the strains. All operations were done under sterile condition in a clean bench. The inoculated test-tube were incubated in a growth chamber at 20 ± 2⁰C and 60-70% relative humidity (RH). After completion of the mycelium run, this culture was used for inoculation of mother culture. Mother culture was prepared on maize grain. The grain was put into water for an hour, cooked for 40 minutes and washed in flowing water. The excess water of the grain drained off. The grain was supplemented with 0.2% CaCO₃ and was mixed manually. The moisture could be of 60-65%. Polypropylene bags of 7″ x 10″ size were filled with 200 g maize and their mouths were plugged by inserting water absorbing cotton with the help of plastic neck. The bags were autoclaved at 121⁰C and 1kg/cm² pressure for 2 hours. After autoclaving and cooling, the bags were inoculated with the pure culture of Pleurotus cystidiosus mushroom strains at the rate of 1 cm² culture slant per packet. After inoculation, the packets were incubated in the laboratory at about 20 ± 2⁰C temperature. Sawdust was thoroughly mixed with nutrient materials, wheat bran at the ratio of 2:1, water was added to make the moisture content 60% and CaCO₃ was added at the rate of 0.2% of the total mixture. Polypropylene bags of 7″ x 10″ size were filled with 500 g mixture of substrate and their mouths were plugged by inserting water absorbent cotton with the help of plastic neck. The bags were autoclaved at 121⁰C and 1kg/cm² pressure for 2 hours. After autoclaving and cooling, the bags were inoculated with the mother culture of Pleurotus cystidiosus mushroom strains at the rate of 1 teaspoonful per packet. After inoculation, the packets were incubated in the laboratory at about 20 ± 2⁰C temperature. After completion of mycelium running, spawn packets were opened by ‘D’ shape cut on both the shoulder of the packets and transferred to culture house at 14-20⁰C and 60-70% relative humidity. Data were analyzed followed by Gomez and Gomez (1984) using MSTAT-C computer program. Means were computed following Duncan’s multiple Ranges Test (DMRT).

Source of Information: Bangladesh J. Mushroom, 5 (1): 43-50, 2011 (June)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The result is almost similar who reported that oyster mushroom took 4 to 8 days for initiation of primordia. It was also similar with the findings who reported that Pleurotus cystidiosus took 7 to 8 days for initiation of primordia. The highest number of primordia was observed in pcys-2 (85.25) which was statistically similar to Pcys-1 (77.75) and the lowest number of primordia was observed in pcys-6 (1.50). The result was found similar who observed that the highest number of primordial/ packet from 3 flushes was 81 in case of oyster mushroom. The highest numbers of fruiting bodies were found in pcys-2 (45.50) which was statistically similar to Pcys-1 (43.00) and the lowest numbers of fruiting bodies were found in pcys-6 (1.50). The highest numbers of effective fruiting bodies were found in pcys-1 (37.25) followed by pcys-2 (35.50) and the lowest numbers of fruiting bodies were found in pcys-6 (1.50). The weight of individual fruiting body was highest in pcys-6 (26.88g) and lowest in pcys- 3 (4.78g) followed by pcys-4 (4.92g), pcys-2 (4.95g), pcys-1 (5.43g) and pcys-5 (6.34g). The highest length of stipe was found in pcys-1 (4.95 cm) and the lowest was found in pcys-3 (1.33 cm). The highest diameter of pileus was found in pcys-6 (9.75 cm) and the lowest diameter of pileus was found in pcys-3 (5.28 cm). The highest thickness was found in pcys-1 (1.35 cm) and the lowest thickness was found in pcys-3 (0.49 cm). Significant variation was observed in biological yield at 5% level of significance. The biological yield from 3 flushes (except in pcys-6) ranged from 37.25 g to 196.3 g/packet. The highest biological yield was found in Pcys-1 (196.3 g/packet) and the lowest biological yield was found in Pcys-6 (37.25 g/packet). Similar trend was observed in economic yield. Remarkable variation was observed in biological efficiency of six strains of Pleurotus cystidiosus. The highest biological efficiency (98.13%) was obtained from Pcys-1 followed by Pcys-2, Pcys-4 and Pcys-3. The lowest biological efficiency (18.63%) was found in Pcys-6.

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