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Effect of Different Supplements to Different Substrates on Growth and Yield of Pleurotus Citrinopileatus

Mahbuba Moonmoon
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh.

Sabina Yesmin
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh.

Md. Nazim Uddin
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh.

Manirul Shaheen
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh.

Nirod Chandra Sarker
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh.

Saleh Ahmed
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh.

Abstract/ Executive Summary:

To find out an effective supplement, Pleurotus citrinopileatus was grown on sawdust (SD), cotton waste (CW) and paddy straw (PS) and their combinations supplemented with rice bran (RB), wheat bran (WB), maize powder (MP) and sesame oil seed cake (SOSC) at 1% level. The days required from opening to first harvest (DROFH) varied from 3.00 to 15.50 days and it was maximum (15.50) in SD+CW (1:1) supplemented with SOSC. The minimum DROFH (3.00) was required in SD+PS (1:1) supplemented with SOSC. The maximum yield (166.00g/ 200g dry substrate) was obtained from SD supplemented with SOSC and the lowest yield (24.00g/200g dry substrate) was obtained from SD supplemented with MP. The highest biological efficiency (BE) (83.0%) was recorded in SD supplemented with SOSC that was significantly higher than all other treatments. The lowest BE (12.0%) was recorded in SD supplemented with MP.

Keywords: Supplements, Pleurotus citrinopileatus, Yield, Biological efficiency

Location: National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka

Start Date: 00-06-2009

End Date: 00-11-2009

Program Area: Crop-Soil-Water Management

Commodity: Mushroom

Objectives:

To investigate the best supplement on the growth and yield of P. citrinopileatus.

Methodology:

The experiment was conducted at the National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka during the months of June to November 2009. Saw dust (SD), cotton waste (CW) and paddy straw (PS) were used as substrates. The straw was cut into small pieces (3-4 cm) and all the substrates were supplemented with 1% rice bran (RB), wheat bran (WB), maize powder (MP) and sesame oil seed cake (SOSC). Water was added to make the moisture content 60% and CaCO³ was added at the rate of 0.2% of the total mixture. Polypropylene bags of 7″×10″ size were filled with 200g of dry substrate mixture and their mouth were plugged by absorbent cotton and covered with brown paper and tied with a rubber band. Then bags were autoclaved at 121? and 15 PSI for 2 hours and then allowed to cool. Each spawn packet was inoculated with the mother culture at the rate of two teaspoonfuls per packet. Bags were then incubated for mycelium running at 25±2? temperature. After completion of mycelium running, the polypropylene bags were cut open on both the side by D-shaped (1.5″×0.5″ ) and were sprayed with water using a sprayer to maintain high relative humidity in the culture house. The spray of water was discontinued a day before the harvest of the fruiting bodies. The experiment was laid out in completely randomized designs (CRD) with 4 replications. Data on days required from opening to first harvest, biological yield and biological efficiency were collected and analyzed using MSTAT-C computer Programme . Means were separated by Duncan’s Multiple Range Test (DMRT) using the same computer program.

Source of Information: Bangladesh J. Mushroom. 4 (1): 39-43, 2010 (June)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The minimum DROFH (3.00) was required in SD+PS (1:1) supplemented with SOSC. The maximum yield (166.00g/ 200g dry substrate) was obtained from SD supplemented with SOSC and the lowest yield (24.00g/200g dry substrate) was obtained from SD supplemented with MP. The highest biological efficiency (BE) (83.0%) was recorded in SD supplemented with SOSC that was significantly higher than all other treatments. The lowest BE (12.0%) was recorded in SD supplemented with MP.

Knowledge Management: Journal