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Seasonal and Sex Related Haematological Variations of the Freshwater Spiny Eel, Mastacembelus Armatus (lacepede) Reared in the Cemented Cisterns

M.Mamnur Rashid
Department of Aquaculture, Bangladesh Agricultrural University, Mymensingh 2202, Bangladesh

S.M. Rahmatullah
Department of Aquaculture, Bangladesh Agricultrural University, Mymensingh 2202, Bangladesh

Abstract/ Executive Summary:

The seasonal and sex related hematological variations in the freshwater spiny eel Mastacembelus armatus were studied during summer from April to June 2001 and winter from November 2001 to January 2002. In total 60 fish (30 males and 30 females) ranging from 30-55.6 cm in total length and from 55-95.5g in weight were collected from the culture stock maintained in the cemented cisterns. It was observed that the values of the total crythorocyte count (TEC), haemoglobin concentration (Hb), haematocrit values, packed cell volume (PCV) and neutrophil count were significantly higher (P<0.05) in males than the females during summer (April to June on-set of breeding season). Females were observed to have remarkably higher values (P<0.01) of erythrocyte sedimentation rate (ESR), total leucocyte count (TLC) and thrombocyte count during summer. On the other hand, insignificant variations in the various hematological parameters were noticed during winter (November to January) between the two sexes of M. armatus.

Keywords: Haematology, Breeding season, M.armatus

Location: BAU

Start Date: 00-04-2001

End Date: 00-02-2002

Program Area: Animal Health and Management

Commodity: Eel

Objectives:

To explore some important haematological parameters of freshwater spiny ell, M. armatus and the variation of those parameters in relation to sex of fish and season.

Methodology:

Live specimens of freshwater spiny eel, Mastacembelus armatus, 30 to 55.6 cm total length and 55 to 95.5 g weight, were obtained from the culture stock maintained in cemented cisterns of the Bangladesh Agricultural University (BAU) Mymensingh, Bangladesh, during summer (April to June) and winter (December 2001 to February 2002). Five fish of either sex were used every month. For anaesthetizing the fish, mixture of clove oil: ethanol: water was used in the ratio of 2:18:9980 (0.02%). After anaesthetizing the fish, blood samples were collected from the caudal vein with a sterile disposable plastic syringe coated with 3.6% sodium citrate as an anticoagulan. To avoid contamination with mucus and water, the area of insertion of needle was wiped with cotton soaked in 70% alcolhol before and after the blood collection. Collected blood was gently pushed into a sterilized small glass vial containing anticoagulant (potassium salt of ethylenediamine tetra-acetic acid, EDTA) to give a final concentration of 5mg EDTA per ml of blood. Erythorocytes (RBC) and leucocytes (WBC) counts were determined by Neubauer’s double haemocytometer using Hayem;s and Shaw’s diluting fluids, respectively. Erythrocyte sedimentation rate (ESR) and packed cell volume (PCV) were measured by Wintrobe’s haematocrit tube. The collected blood was inserted into dry Wintrobe’s tube by Pasteur pipette exactly up to 0 or 100 mark. Care was taken not to allow any bubble in the tube. The tube was placed in a special rack in vertical position for 1 hour. ESR was calculated by measuring the distance the erythrocyte had sedimented in the scale from the top of the tube and the result was expressed in mm/h. The tube was then spun in haematocrit centrifuge at 3000 rpm for 30 minutes. The haemoglobin concentration of blood was evaluated by Sahli’s haemocytometer. Erythrocyte indices or absolute values viz. mean cell haemoglobin concentration (MCHC) and mean cell volume (MCV) were calculated mathematically from the erythrocyte count, haemoglobin concentration and haematocrit. Differential cell counts were made from the thin blood films prepared on glass slides immediately after collection of the blood samples, which were air dried, stained with Wright and Giemsa stain, washed in running tap water and dried followed by mounting in Canada balsam. A total of 100 leucocytes were counted under microscope at x 45 for each sample and the percentage of different types of leucocytes was calculated. Statistical analysis: The mean values of each parameter at each sampling were compared using analysis of variance (ANOVA) technique through complete randomized design (CRD).

Source of Information: Bangladesh J. Fish., Res., 6(2): 141-147, 2002

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The physiological susceptibility and pathobiology of the spiny ell M. armatus in response to any physico-chemical or biological factors might be similar to other fishes.

Knowledge Management: Journal