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Md. Alamgir Kabir
Department of Fisheries, Faculty of Biological Sciences, University of Dhaka, Dhaka-1000, Bangladesh

Mohammad Shamsur Rahman
Department of Fisheries, Faculty of Biological Sciences, University of Dhaka, Dhaka-1000, Bangladesh

Anwar Hossain
Department of Fisheries, Faculty of Biological Sciences, University of Dhaka, Dhaka-1000, Bangladesh

Shankar Chandra Mandal*
Department of Fisheries, Faculty of Biological Sciences, University of Dhaka, Dhaka-1000, Bangladesh

Proximate composition and microbial quality of three aquarium fish feeds commonly found in Bangladesh were collected from fish feed market of Dhaka city. Significantly higher moisture content (2.12 ± 0.06%) was found in Super Nova TM fish feed while similar moisture content was detected in other two feeds (p<0.05). On the other hand ash content detected in OptimumTM and Osaka 2000 TM aquarium fish feed was significantly higher than that of Super NovaTM (10.67±0.19%) (p<0.05).Protein content found in OptimumTM (42.21 ± 0.12%) was significantly higher than that of Osaka 2000 TM and Super NovaTM (p<0.05). However, similar lipid content was found in Optimum TM (1.06 ± 0.03%) and Super NovaTM (1.13 ± 0.01%) aquarium fish feed which were significantly higher than that of Osaka 2000 TM (p<0.05). The Escherichia coli and Staphylococcus aureus were found in all feeds. However Vibrio sp was not found in any feed. Total bacterial count was significantly higher in Osaka 2000 TM feed than that of others (P<0.05). However S. aureus was significantly higher in Super NovaTM than that of others while E. coli count was significantly higher in OptimumTM fish feed than that of others (p<0.05). Nutritional quality of the aquarium fish feed was qualified to some extent to meet the minimum requirement of the aquarium fish while microbial quality indicates some degrees of deterioration which may cause diseases of the aquarium fishes. Thus proper storage condition, hygienic handling and processing should be maintained to prevent the microbial contamination in aquarium fish feeds. There should be a monitoring cell from the government to assess the quality of this expensive imported feeds for aquarist.

  Biochemical composition, Microbial quality, Aquarium fish feed
  Aquarium fish feed market in Kataban, Dhaka
  00-06-2014
  
  Quality and Nutrition
  Aquatic animal

To assess the biochemical composition and microbial quality of aquarium fish feed commonly found in Bangladesh.

Collection and preparation of samples: Three commercial aquarium fish feed samples of Super Nova TM , Osaka 2000 TM and Optimum TM were obtained from the aquarium fish feed market in Kataban, Dhaka in June 2014. They are all imported. Various information labeled in the three aquarium fish feed and the biochemical composition as labeled by the manufacturer have shown. The biochemical composition and microbial quality of the samples were analyzed in the laboratory of Department of Fisheries, University of Dhaka by using Standard methods. For the determination of moisture content, the initial weights were taken first and then samples were dried in an oven at about 105 ºC for about 10 to 18 hrs until a constant weight was reached and cooled in desiccators and weights of the samples were taken again. Ash content was estimated as inorganic residue left over after the organic matter has been burnt away at about 600-700 ºC. The sample was first burnt on a flame until it became charred and then in muffle furnace at about 600-700 ºC till the residue become white. The percentage of nitrogen in the sample was calculated as % of Protein = {(S-B) × 2.9 × Moisture factor}/Weight of sample; where, S = Titration reading for sample, B = Titration reading for blank and Moisture factor = (100-Moisture content)/100. Then 5-10 mL of the extract was taken in 25 mL beaker and allowed to air dry and then dried in an oven at 60 ºC for the determination of total lipid was determined gravimetrically. Nutrient agar was used to determine the total bacterial count. One gram sample was taken in 9 mL sterile normal saline to prepare a 10 -1 dilution. For the isolation and enumeration of S. aureus, 100 µL serially diluted samples was spread onto the manitol salt agar plates and incubated at 37 °C for 18 to 24 hours. Golden colonies were counted as S. aureus. The means of different parameters were compared using ANOVA followed by Tukey’s post hoc for multiple comparisons. Statistical software SPSS version 20 was used to analyze the data with the level of significance at p<0.05.

  Bangladesh J. Zool. 42(2): 283-294, 2014
  
Funding Source:
  

It has been found that nutritional quality and microbial load of the examined fish feeds differed with the values supplied by the manufacturer. Aquarists get losses by using these types of feeds in rearing ornamental fishes. Thus, government should take proper initiative in quality control for the benefit of the aquarium fish industry in the country.

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