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Different Levels of Nitrogen and Phosphorus with or Absence of Vam Fungal Inoculum on Rice (oryza Sativa L)

M.A. Kalam
Soil Science Division, Bangladesh Institute of Nuclear Agriculture (BINA)

M.B. Hossain
Soil Science Division, Bangladesh Institute of Nuclear Agriculture (BINA)

T. Sarmin
Soil Science Division, Bangladesh Institute of Nuclear Agriculture (BINA)

A.Z.M. Moslehuddin
Department of Soil Science, Bangladesh Agricultural University, Mymensingh

M.M.K. Khan
Soil Science Division, Bangladesh Institute of Nuclear Agriculture (BINA)

Abstract/ Executive Summary:

Pot experiment was conducted at BINA headquarter, Mymensingh, Bangladesh to evaluate the response of different levels of nitrogen and phosphorus containing fertilizers with or without VAM fungal on rice. The treatment combinations were N₀P₀, N₀P₂₀, N₀P₄₀, N₁₀₀P₀, N₁₀₀P₂₀, N₁₀₀P₄₀, N₂₀₀P₀, N₂₀₀P₂₀ & N₂₀₀P₄₀ with or without VAM fungal inoculum. Experiment was laid out in a completely randomized design with four replications. Results on root and shoot dry weights were significantly affected by the treatments at different growth stages of plant. At 40 and 95 DAT, maximum shoot dry weight (9.75 and 24.33 g hill^-1) and root dry weight (2.95 and 3.23 g hill^-1) were observed in N₂₀₀P₄₀ + VAM fungal treatment. The minimum shoot and root dry weight was found in N₀P₂₀ treatment. The maximum root infection (74.33%) was observed in N₂₀₀P₀ + VAM which was statistically similar to N₁₀₀P₀ + VAM and N₁₀₀P₂₀ + VAM. The highest plant height, number of tillers per hill, panicle length, filled grain per panicle and 1000 seed weight was obtained from N₂₀₀P₄₀ + VAM. Unfilled grain had negative and significant correlation with number of tiller, panicle length and filled grain of rice suggesting it lower number of unfilled grain helped to increase rice yield. Number of tiller per hill showed positive and significant correlation with grain yield of rice in N₂₀₀P₄₀ + VAM treatment. Maximum grain yield (4.45 t ha^-1) was observed in N₂₀₀P₄₀ + VAM which was statistically similar to N₂₀₀P₂₀ + VAM treatment. In conclusion, application of VAM inoculum may be stimulated growth and yield of rice as compared to uninoculated plant.

Keywords: VAM, Mycorrhiza, Rice, Different levels of N and P, Root infection, Yield

Location: BINA headquarter, Mymensingh

Start Date: 00-11-2010

End Date: 00-03-2011

Program Area: Crop-Soil-Water Management

Commodity: Bio fertilizer

Objectives:

1.To determine the effect of different levels of nitrogen and phosphorus with and without VAM inoculum on root and shoot development at different growth stages, mycorrhizal root infection, yield contributing parameters and yield of rice.

Methodology:

Pot experiment was conducted at BINA headquarter, Mymensingh to evaluate the response of different levels of nitrogen and phosphorus containing fertilizers with or without VAM fungal on vegetative growth at different stages, mycorrhizal root infection, yield contributing parameters and yield of rice. Initial soil samples were randomly collected, air dried and passed through a 2 mm sieve for analyzing some important physicochemical properties. The experimental soils were sandy loam in texture containing 0.46% organic carbon, 0.06% total nitrogen, 14 ppm available phosphorus, 0.13 ppm exchangeable potassium and a pH of 6.9 in H₂O. The treatment combinations were N₀P₀, N₀P₂₀, N₀P₄₀, N₁₀₀P₀, N₁₀₀P₂₀, N₁₀₀P₄₀, N₂₀₀P₀, N₂₀₀P₂₀ & N₂₀₀P₄₀ with or without VAM inoculum. Treatments were replicated thrice and arranged in a completely randomized design. Total number of pots (54) was used in this experiment. A ten kg soils was placed into 20 × 15 × 15 cm earthen pot. Forty two kg K₂O ha^-1 as muriate of potash and 10 kg S ha^-1as gypsum were applied as basal dose. According to treatments combination one third of nitrogen and full dose of phosphorus as urea and triple super phosphate were applied with basal dose of potassium and sulphur containing fertilizers, respectively. Three seedlings of Oryza sativa L (cv. BINAdhan 6) were grown in each pot. VAM spores were collected from rice, marigold, mungbean and peanut rhizosphere soils. This inoculum was covered with a soil layer of 2 cm in which rice seedlings were transplanted. The rest nitrogen as urea was applied in two splits at maximum vegetative phage and panicle initiation stage of rice. Cultural practice was done whenever necessary. At 40 and 95 DAT (days after transplanting), single plant was uprooted from the experimental pot. Root and shoot were separated with the help of a sharp scissors. Samples of shoot and root portions of plant at vegetative stages, grain and straw at maturity were sampled for dry weight estimation. For root infection study, fine roots were collected and washed with water. The washed roots were cut into 1 cm segments and thoroughly mixed. This mixed sample was bleached with 10 per cent (w/v) KOH at 90⁰C for 2 h and stained with trypan blue. The presence or absence of infection was calculated as follows: Number of VAM positive segments Root infection (%) = (No of VAM positive segments/Total no of segemnts) × 100. Total number of segments Shoot and root dry weight of plant were measured at 40 and 95 DAT. Data on yield contributing parameters and grain yield of rice was computed on 14 per cent moisture basis. Data were statistically analyzed and Duncan’s Multiple Range was applied to examine significant differences between the treatment means.

Source of Information: J. Agrofor. Environ. 5 (2): 7-10, 2011; ISSN 1995-6983

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Results on root and shoot dry weights were significantly affected by the treatments at different growth stages of plant. At 40 and 95 DAT, maximum shoot dry weight (9.75 and 24.33 g hill^-1) and root dry weight (2.95 and 3.23 g hill^-1) were observed in N₂₀₀P₄₀ + VAM fungal treatment. The minimum shoot and root dry weight was found in N₀P₂₀ treatment. The maximum root infection (74.33%) was observed in N₂₀₀P₀ + VAM which was statistically similar to N₁₀₀P₀+ VAM and N₁₀₀P₂₀ + VAM. The highest plant height, number of tillers per hill, panicle length, filled grain per panicle and 1000 seed weight was obtained from N₂₀₀P₄₀ + VAM. Unfilled grain had negative and significant correlation with number of tiller, panicle length and filled grain of rice suggesting it lower number of unfilled grain helped to increase rice yield. Number of tiller per hill showed positive and significant correlation with grain yield of rice in N₂₀₀P₄₀ + VAM treatment. Maximum grain yield (4.45 t ha^-1) was observed in N₂₀₀P₄₀ + VAM which was statistically similar to N₂₀₀P₂₀ + VAM treatment. In conclusion, application of VAM inoculum may be stimulated growth and yield of rice as compared to uninoculated plant.

Knowledge Management: Journal