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Mycelial Growth at Different Temperature and Ph and Yield on Different Substrates of Oyster Mushroom Var. White Snow

Tasnima Mahjabin
National Mushroom Development and Extension Centre, Sobhhanbag, Savar, Dhaka, Bangladesh.

Nirod Chandra Sarker
National Mushroom Development and Extension Centre, Sobhhanbag, Savar, Dhaka, Bangladesh.

Abdus Salam Khan
National Mushroom Development and Extension Centre, Sobhhanbag, Savar, Dhaka, Bangladesh.

Mahbuba Moonmoon
National Mushroom Development and Extension Centre, Sobhhanbag, Savar, Dhaka, Bangladesh.

A. J. Kakon
National Mushroom Development and Extension Centre, Sobhhanbag, Savar, Dhaka, Bangladesh.

Taufiqur Rahman
National Mushroom Development and Extension Centre, Sobhhanbag, Savar, Dhaka, Bangladesh.

Saleh Ahmed
National Mushroom Development and Extension Centre, Sobhhanbag, Savar, Dhaka, Bangladesh.

Abstract/ Executive Summary:

To study the effect of different temperature and pH on mycelial growth and substrates on yield of oyster mushroom var. white snow, six different temperatures- 10 ⁰C, 15 ⁰C, 20 ⁰C, 25 ⁰C, 30 ⁰C and 35 ⁰C, ten different pH levels- 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5 and 9.0 and eight different substrates- sawdust, coirpith, lentil straw, chickpea straw, lentil + chickpea straw, wheat straw, rice straw (autoclaved) and rice straw (hot water treated) were tested. The highest mycelium growth rate was observed at 20 ⁰C temperature and 6.5 pH level and the highest yield (242.8 g/ 500g substrate) obtained from hot water treated rice straw.

Keywords: Temperature, pH, Substrate, Number of fruiting body, Yield

Location: National Mushroom Development and Extension Centre, Savar, Dhaka

Start Date: 00-09-2010

End Date: 00-11-2010

Program Area: Crop-Soil-Water Management

Commodity: Mushroom

Objectives:

To study the mycelial growth in different temperature and pH for yield and yield attributes on different substrates of the mushroom.

Methodology:

The present investigation was conducted at National Mushroom Development and Extension Centre, Savar, Dhaka from September to November 2010. Potato dextrose agar (PDA) medium was in use to evaluate the suitable temperature and pH for mycelium growth of oyster mushroom var. white snow. The culture media was prepared. For suitable temperature determination, the composition was adjusted at pH value 6.5 before autoclave. After cooling, the petriplates were inoculated with the inoculum of white snow mushroom. The inoculated plates were incubated at 10 ⁰C, 15 ⁰C, 20 ⁰C, 25 ⁰C, 30 ⁰C and 35 ⁰C temperatures. For pH study, ten different pH levels such as 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5 and 9.0 of the media were tested for getting the best for this particular mushroom. The pH levels were adjusted by adding 1N HCl or NaOH before autoclaving. Eight different agricultural wastes such as saw dust, coirpith, lentil straw, chickpea straw, lentil + chickpea straw, wheat straw, rice straw (autoclaved) and rice straw (hot water treated) were tested as substrates. Spawn packets of all the substrates except the rice straw (hot water treated) were prepared, inoculated and incubated following the procedure mentioned. The hot water treated rice straw was prepared. All the spawn packets were of 500 g. After completion of mycelium running, spawn packets were opened by D shaped cut on the shoulder and removed the pp sheet. The relative humidity and temperature of the culture house were maintained at 80-90% and 20-25 ⁰C respectively by spraying water. The experiment was laid out following completely randomized design (CRD) with four replications. Data on mycelial growth rate, days to completion of mycelium running, days required from opening to first harvest, length and diameter of pileus, numbers of fruiting body and yield were recorded and analyzed following standard methods using MSTAT-C computer program. Means were computed following DMRT using the same computer program.

Source of Information: Bangladesh J. Mushroom. 5 (1): 29-33, 2011 (June)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The present results revealed that the highest mycelium growth rate was recorded at 20 ⁰C with 6.5 pH. On the other hand, the minimum days required for mycelium running and the maximum yield (242.8 g/packet) obtained from hot water treated rice straw in oyster mushroom var. white snow.

Knowledge Management: Journal