Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Performance of Different Strains of Pleurotus Cystidiosus Under Bangladesh Condition

Kysun Rafat Howlader
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh.

Abdus Salam Khan
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh.

Mahbuba Moonmoon
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh.

A. J. Kakon
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh.

Saleh Ahmed
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh.

Nirod Chandra Sarker
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh.

Abstract/ Executive Summary:

Performance of different strains of Pleurotus cystidiosus (Pcys) were studied in National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh. Significant variation was observed in the growth, yield and yield contributing characters of these strains. The highest mycelial growth (0.58 cm/day) was observed in Pcys-4 which was followed by Pcys-5, Pcys-2 and Pcys-1. The lowest mycelial growth (0.22 cm/day) was observed in Pcys-3. The highest biological and economic yield, 196.3 and 189.0 g/packet respectively were obtained from Pcys-1, which followed by Pcys-2, Pcys-4 and Pcys-5. The lowest biological and economic yields were observed in Pcys-6. The number of effective fruiting bodies was the highest (37.25) in pcys-1, while, the weight of individual fruiting body was the highest (26.88 g) in Pcys-6. The highest length of stipe (4.95cm) and thickness of pileus (1.35cm) were observed in Pcys-1. The strain also showed the highest biological efficiency (BE) among the strains.

Keywords: Pleurotus cystidiosus, Growth, Yield attributes, Yield.

Location: National Mushroom Development and Extension centre (NAMDEC), Sobhanbag, Savar, Dhaka

Start Date: 00-11-2010

End Date: 00-02-2011

Program Area: Variety and Species

Commodity: Mushroom

Objectives:

To identify the best strain that can be highly productive and suitable for the cultivation in the winter in Bangladesh.

Methodology:

The experiment was conducted in the National Mushroom Development and Extension centre (NAMDEC), Sobhanbag, Savar, Dhaka during November 2010 to February 2011. Six different strains of Pleurotus cystidiosus (Pcys) available in NAMDEC were selected for the experiment. The strains were designated as Pcys-1, Pcys-2, Pcys-3, Pcys-4, Pcys-5 and Pcys-6. Pure cultures of selected strains were grown on potato dextrose agar (PDA) medium. The medium wa s poured into test-tube. The medium in test-tube was autoclaved at 120 ⁰C and 1 kg/cm 2 for 20 minutes. After sterilization and solidification, the test-tubes were inoculated with the inocula of the strains. All operations were done under sterile condition in a clean bench. The inoculated test-tube were incubated in a growth chamber at 20 ± 2 ⁰C and 60-70% relative humidity (RH). After completion of the mycelium run, this culture was used for inoculation of mother culture. Mother culture was prepared on maize grain. The grain was put into water for an hour, cooked for 40 minutes and washed in flowing water. The excess water of the grain drained off. The grain was supplemented with 0.2% CaCO₃ and was mixed manually. The moisture could be of 60-65%. Polypropylene bags of 7″ x 10″ size were filled with 200g maize and their mouths were plugged by inserting water absorbing cotton with the help of plastic neck. The bags were autoclaved at 121 ⁰C and 1kg/cm² pressure for 2 hours. After autoclaving and cooling, the bags were inoculated with the pure culture of Pleurotus cystidiosus mushroom strains at the rate of 1 cm 2 culture slant per packet. After inoculation, the packets were incubated in the laboratory at about 20 ± 2 ⁰C temperature. Sawdust was thoroughly mixed with nutrient materials, wheat bran at the ratio of 2:1, water was added to make the moisture content 60% and CaCO₃ was added at the rate of 0.2% of the total mixture. Polypropylene bags of 7″ x 10″ size were filled with 500 g mixture of substrate and their mouths were plugged by inserting water absorbent cotton with the help of plastic neck. The experiment was laid out in a completely randomized design (CRD) with four replications. Yield and yield parameters were taken on the basis of three flashes except in pcys-6 where only one flash was obtained. Data on mycelial growth rate, days required from stimulation to primordia initiation, number of primordia, number of fruiting body, number of effective fruiting body, length of stipe, diameter of stipe, diameter of pileus, thickness of pile us, biological yield (g/packet) and economic yield (g/packet) were recorded. Data were analyzed followed by Gomez and Gomez using MSTAT-C computer program. Means were computed following Duncan’s multiple Ranges Test (DMRT).

Source of Information: Bangladesh J. Mushroom. 5 (1): 43-50, 2011 (June)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The number of effective fruiting bodies was the highest (37.25) in pcys-1, while, the weight of individual fruiting body was the highest (26.88 g) in Pcys-6. The highest length of stipe (4.95cm) and thickness of pileus (1.35cm) were observed in Pcys-1. The strain also showed the highest biological efficiency (BE) among the strains.

Knowledge Management: Journal