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Oyster Mushroom (pleurotus Ostreatus) Improves Glycemic Status of Normotensive Diabetic Male Volunteers of Bangladesh

Md. Bazlul Karim Choudhury
Ph.D. Student
Jahangirnagar University and OSD, DG Health, Mohakhali, Dhaka-1212, Bangladesh.

A. J. Kakon
National Mushroom Development and Extention Center, Sobhanbag, Savar, Dhaka, Bangladesh.

Taufiqur Rahman
National Mushroom Development and Extention Center, Sobhanbag, Savar, Dhaka, Bangladesh.

Ferdousi Rahman Mowsumi
National Mushroom Development and Extention Center, Sobhanbag, Savar, Dhaka, Bangladesh.

Md. Burhan Uddin
Department of Pharmacology, Jalalabad Ragib-Rabeya Medical College, Sylhet.

M. Shahabuddin Kabir Choudhuri
Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, Bangladesh.

Md. Shahdat Hossain
Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka, Bangladesh.

Abstract/ Executive Summary:

The study was undertaken to investigate the effect of Pleurotus ostreatus on glycemic status of normotensive diabetic male volunteers. The human fasting plasma level of creatinine and glucose associated with glycated hemoglobin (HbA1c) were estimated before and three months after administration of mushroom capsule. The feeding of three grams mushroom capsule daily showed no significant effect on plasma creatinine level (p = 0.903) but there was significant reduction of fasting plasma glucose (p = 0.001). There was also significant reduction of glycated hemoglobin (p = 0.000). All of these findings suggest that Pleurotus ostreatus may able to improve diabetic status significantly without interfering the renal function.

Keywords: Diabetes, Pleurotus ostreatus, Plasma glucose, HbA1c.

Location: Laboratory of Strengthening Mushroom Development Project, National Mushroom Development and Extension Center (NAMDEC), Sobhanbag, Savar, Dhaka.

Start Date: 00-04-2010

End Date: 00-05-2011

Program Area: Quality and Nutrition

Commodity: Mushroom

Objectives:

To evaluate efficacy of oyster mushroom (Pleurotus ostreatus) on glycemic control and improving quality of life in type 2 diabetic patients.

Methodology:

The experiment was conducted between April 2010 and May 2011 in the laboratory of Strengthening Mushroom Development Project, National Mushroom Development and Extension Center (NAMDEC), Sobhanbag, Savar, Dhaka. In the study 27 normotensive male subjects (systolic BP ranged from 90-130 mmHg and diastolic BP ranged from 60-85 mmHg) aged (years) from 28 to 77, suffering type 2 diabetes and resided at Savar were considered. After getting written consent, the subjects were included. The details history was taken from the subjects including age, sex, occupation, educational status, marital status, family history and drug history. During the study any acute or chronic disease or medication, malabsorption and any addiction except cigarette smoking were excluded. Female subjects were not included in the study. At the beginning of study, health status was being evaluated of the subjects. Fasting blood sample was collected for analysis. Mushroom capsules were supplied to take two capsules three times daily. Each capsule contains 500 mg Pleurotus ostreatus powder, so that each subject took 3 gms mushroom powder daily. After three months the subjects were evaluated and all the investigation procedures were repeated. If any drug previously getting by the subjects, it was continued. 10 ml fasting blood sample was collected from median cubital vein with all aseptic precaution. After collecting blood, immediately it was poured into test tube containing fluoride and EDTA. The test tube then gently shaken so that anti coagulant and fluoride mix with the blood properly. Then it was centrifuged by 3000 rpm for 5 minutes. Plasma was separated which were transferred into two eppendorf containing 1 ml in each. All the tests were carried out as early as possible. Fresh fruiting body of Pleurotus ostreatus was collected from culture house of National Mushroom Development and Extension Centre. Blood pressure was measured using sphygmomanometer by trend physician. Cuff of the sphygmomanometer was binded on upper two third of the right arm of the subjects. Plasma creatinine was estimated to detect renal impairment by using ‘alkaline picrate’ method. Plasma glucose level was estimated using enzymatic ‘Glucose oxidase method’. By using commercially available reagent kit, analysis was done by semi auto biochemical analyzer 3000 evaluation. Glycated hemoglobin was estimated by a photometric method using ‘Stanbio reagent kit’. In the method a preparation of hemolyzed whole blood was mixed with a weakly binding cation-exchange resin. The non-glycosylated hemoglobin (HbA 0) binded to the resin, leaving (HbA1) free which were removed by means of a resin separator in the supernate. The percent of HbA1 was determined by measuring the absorbance values at 415 nm of the HbA1 fraction and of the total Hb fraction, calculating the ratio of absorbances (R), and comparing this ratio to that of a glycohemoglobin standard carried through the same procedure. Results were expressed as HbA1 but it was converted as HbA1c by using a conversion factor. Results were expressed as mean ± SE. Paired Student’s ‘t’ test was used to see the level of significance. 95% confidence limit was taken as level of significance.

Source of Information: Bangladesh J. Mushroom. 5(2): 1-6, 2011 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The blood glucose and triglyceride (TG) lowering effects of water soluble extracts from Lentinus edodes, Pleurotus ostreatus and Phellinus linteus in the streptozotocin-induced diabetic model have been clearly demonstrated. Such results strongly suggest that these mushrooms have potential preventive and therapeutic action in diabetes mellitus. Our findings are also supportive with them. But further studies with large sample size are needed to verify these observations. In conclusion the results throw light on the potential use of oyster mushroom for better glycemic control and better quality of life.

Knowledge Management: Journal